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崔艳芳, 王凤杰, 余磊, 叶华虎, 杨贵波. 2019: 恒河猴和食蟹猴直肠微生物菌群的宏基因组比较. 动物学研究, 40(2): 89-93. DOI: 10.24272/j.issn.2095-8137.2018.061
引用本文: 崔艳芳, 王凤杰, 余磊, 叶华虎, 杨贵波. 2019: 恒河猴和食蟹猴直肠微生物菌群的宏基因组比较. 动物学研究, 40(2): 89-93. DOI: 10.24272/j.issn.2095-8137.2018.061
Yan-Fang Cui, Feng-Jie Wang, Lei Yu, Hua-Hu Ye, Gui-Bo Yang. 2019: Metagenomic comparison of the rectal microbiota between rhesus macaques (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis). Zoological Research, 40(2): 89-93. DOI: 10.24272/j.issn.2095-8137.2018.061
Citation: Yan-Fang Cui, Feng-Jie Wang, Lei Yu, Hua-Hu Ye, Gui-Bo Yang. 2019: Metagenomic comparison of the rectal microbiota between rhesus macaques (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis). Zoological Research, 40(2): 89-93. DOI: 10.24272/j.issn.2095-8137.2018.061

恒河猴和食蟹猴直肠微生物菌群的宏基因组比较

Metagenomic comparison of the rectal microbiota between rhesus macaques (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis)

  • 摘要: 恒河猴(Macaca mulatta)和食蟹猴(Macaca fascicularis)是建立人类疾病动物模型的常用动物。为确定这两种动物间肠道微生物菌群的差异,我们收集了20只恒河猴和21只食蟹猴的直肠拭子,通过16S rRNA基因深度测序对这些拭子中的微生物组成进行了检测。发现食蟹猴直肠微生物菌群的α多样性显著高于恒河猴,尽管观察到的微生物分类单元数(operational taxonomic units, OTUs)几乎相同。两种动物的优势微生物门和属相似,但优势微生物类群的相对丰度在两种动物之间显著不同。对微生物群落功能预测(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States, PICRUSt)分析发现两种动物微生物菌群的功能成分,尤其是脂多糖(LPS)合成蛋白存在显著差异。上述数据表明这两种关系密切的猕猴属动物的肠道微生物菌群在组成和功能上都存在显著差异,需在疾病模型动物选择时予以考虑。

     

    Abstract: Rhesus macaques (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis) are frequently used in establishing animal models for human diseases. To determine the differences in gut microbiota between these species, rectal swabs from 20 rhesus macaques and 21 cynomolgus macaques were collected, and the microbial composition was examined by deep sequencing of the 16S rRNA gene. We found that the rectal microbiota of cynomolgus macaques exhibited significantly higher alpha diversity than that of rhesus macaques, although the observed number of operational taxonomic units (OTUs) was almost the same. The dominant taxa at both the phylum and genus levels were similar between the two species, although the relative abundances of these dominant taxa were significantly different between them. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed significant differences in the functional components between the microbiota of the two species, in particular the lipopolysaccharide (LPS) synthesis proteins. The above data indicated significant differences in microbial composition and function between these two closely related macaque species, which should be taken into consideration in the future selection of these animals for disease models.

     

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