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Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 2016: 香鱼CSF-1R基因的克隆、病理相关性表达及功能分析. 动物学研究, 37(2): 96-102. DOI: 10.13918/j.issn.2095-8137.2016.2.96
引用本文: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 2016: 香鱼CSF-1R基因的克隆、病理相关性表达及功能分析. 动物学研究, 37(2): 96-102. DOI: 10.13918/j.issn.2095-8137.2016.2.96
Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 2016: Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 37(2): 96-102. DOI: 10.13918/j.issn.2095-8137.2016.2.96
Citation: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 2016: Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 37(2): 96-102. DOI: 10.13918/j.issn.2095-8137.2016.2.96

香鱼CSF-1R基因的克隆、病理相关性表达及功能分析

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

  • 摘要: 巨噬细胞集落刺激因子受体(colony-stimulating factor 1 receptor,CSF-1R)是单核/巨噬细胞(MO/MΦ)上的重要调控因子。虽然CSF-1R基因在一些硬骨鱼中已被鉴定,但其功能尚不明确。本文对一种硬骨鱼类-香鱼(Plecoglossus altivelis)的CSF-1R基因进行了研究。多重序列比对和系统进化树分析表明,香鱼CSF-1R和青鳉CSF-1R的进化关系最近。实时荧光定量PCR检测揭示香鱼CSF-1R基因mRNA主要在头肾来源的单核/巨噬细胞(MO/MΦ)、脾和头肾中表达,鳗弧菌感染后,多个组织中CSF-1R基因mRNA表达显著变化。随后我们原核表达并制备了香鱼CSF-1R抗体。抗体封闭48小时后,流式细胞术检测香鱼MO/MΦ对大肠杆菌的吞噬能力显著降低,表明香鱼CSF-1R参与调控MO/MΦ功能。

     

    Abstract: Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.

     

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