Volume 22 Issue 6
Nov.  2001
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ZHA Hong-Guang, ZHANG Yun. Purification of a Phospholipase A[2] from Bungarus fasciatus Venom by One Step Sepharose 4B Method. Zoological Research, 2001, 22(6): 433-436.
Citation: ZHA Hong-Guang, ZHANG Yun. Purification of a Phospholipase A[2] from Bungarus fasciatus Venom by One Step Sepharose 4B Method. Zoological Research, 2001, 22(6): 433-436.

Purification of a Phospholipase A[2] from Bungarus fasciatus Venom by One Step Sepharose 4B Method

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-12-22
  • By one step affinity chromatography (acid treated Sepharose 4B as matrix,tyrode solution containing 0.2 mol/L D-galactose as eluting solution),a phospholipase A[2] was isolated from Bungarus fasciatus venom.Its partial N-terminal sequence is identical to previously reported Bungarus fasciatus venom phospholipase A[2] isozyme Ⅵ (Lu & Lo,1978).This phospholipase A[2] has only weak phospholipase A[2] activity,no hemolytic nor hemorrhage activity.The enzyme exists as monomer and its molecular weight is 14 kDa,and has a relatively high carbohydrate content (13.4% w/w).
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