Volume 19 Issue 5
Sep.  1998
Turn off MathJax
Article Contents
DING Bo, Oliver A.Ryder, ZHANG Ya-ping, ZHANG Jin-guo, ZHANG Cheng-lin, ZHANG Xi-ge. DNA Preparation and Sequencing From Scent marks in The Glant Panda. Zoological Research, 1998, 19(5): 344-349.
Citation: DING Bo, Oliver A.Ryder, ZHANG Ya-ping, ZHANG Jin-guo, ZHANG Cheng-lin, ZHANG Xi-ge. DNA Preparation and Sequencing From Scent marks in The Glant Panda. Zoological Research, 1998, 19(5): 344-349.

DNA Preparation and Sequencing From Scent marks in The Glant Panda

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1998-10-22
  • scent marks secreted by giant pandas may function as communication among individuals.We collected nine scent mark samples from seven giant pandas with different sampling methods.DNAs were successfully prepared with INSTAGENE kit.The mitochondrial DNA Dloop,cytochrome b gene and Thr-tRNA gene regions were amplified and the PCR products were purified and sequenced.Our results suggest that DNA from scent marks can be obtained from samples collected with different methods.A cotton stick is a best sampling way compared with other methods.Our work shows a new simple and efficient DNA source for studying on genetic diversity of giant pandas.
  • loading
  • Relative Articles

    [1] Kai Chen, Yu-Hong Shi, Jiong Chen, Ming-Yun Li. A soluble FcγR homolog inhibits IgM antibody production in ayu spleen cells. Zoological Research, 2019, 40(5): 404-415.  doi: 10.24272/j.issn.2095-8137.2019.056
    [2] Xiu-Xiu Liu, Cheng-Yuan Wang, Chun Luo, Jun-Qing Sheng, Di Wu, Bei-Juan Hu, Jun-Hua Wang, Yi-Jiang Hong. Characterization of cyclophilin D in freshwater pearl mussel (Hyriopsis schlegelii). Zoological Research, 2017, 38(2): 103-109.  doi: 10.24272/j.issn.2095-8137.2017.018
    [3] Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102.  doi: 10.13918/j.issn.2095-8137.2016.2.96
    [4] Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri). Zoological Research, 2013, 34(2): 127-131.  doi: 10.3724/SP.J.1141.2013.02127
    [5] HUANG Yan, LIANG Xu-Fang*, WANG Lin, LI Guang-Zhao, Liu Xiu-Xia, YAO Yu. Molecular Characterization and Evolutional Analysis of Liportein Lipase (LPL) and Hepatic Lipase (HL) Gene in Chinese sturgeon and Other Six Freshwater Fishes. Zoological Research, 2010, 31(3): 239-249.  doi: 10.3724/SP.J.1141.2010.03239
    [6] LIU Si-Qing, ZHANG Jia-Bo, TANG Qiong-Ying, LIU Huan-Zhang. Phylogenetic Relationships Among Cobitoidea Based on Mitochondrial ND4 and ND5 Gene Sequences. Zoological Research, 2010, 31(3): 221-229.
    [7] YANG Xiu-qin, GUO Li-juan, MA Jian-zhang, LIU Di. Cloning, Expression and Variation Analysis of the Wild Boar CAPN7 Gene. Zoological Research, 2009, 30(5): 503-508.  doi: 10.3724/SP.J.1141.2009.05503
    [8] BAI Yin-ba-tu, LUO Fen-hua, HU Tian-yuan, HOU Yue, WU Ying-ji. Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System. Zoological Research, 2009, 30(3): 262-266.  doi: 10.3724/SP.J.1141.2009.03262
    [9] YU Ying, LIANG Xu-fang, LI Guan-gui, WANG Lin, LI Guang-zhao. Molecular Cloning and Analysis of Leptin Gene in Grass Carp (Ctenopharyngodon idellus) and Silver Carp (Hypophthalmichthys molitrix). Zoological Research, 2009, 30(5): 479-485.  doi: 10.3724/SP.J.1141.2009.05480
    [10] LIU Wei-bin, GONG Cheng-liang, XUE Ren-yu, ZHOU Wen-lin, ZHU Xu-xian, CAO Guang-li *. Cloning and Analysis of Fhx/P25 Gene Promoter of Bombyx mori Fibroin Protein. Zoological Research, 2007, 28(1): 17-23.
    [11] GAO Xin-yu, LIU Yang, LIU Ding-zhen , *, WEI Fu-wen. Analysis on the Growth of Bamboo Species Bashania fargesii Selection of Giant Pandas in Qinling Mountains. Zoological Research, 2006, 27(2): 157-162.
    [12] ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
    [13] ZHANG Ming, HOU Rong, LIU Yu-liang, ZHENG Hong-pei, ZHU Qing, ZHANG Zhi-he, XIAN Hong. Effects of Epidermal Growth Factor and Insulin on Biological Characteristics of Giant Panda (Ailuropoda melanoleuca) Cutaneous Fibroblast in Vitro. Zoological Research, 2005, 26(5): 499-505.
    [14] SHI Yan, WU Xiao-bing, YAN Peng, CHEN Bi-hui. Cloning and Sequences Analysis of the Second Exon of MHC Class Ⅱ B Genes in Chinese Alligator,Alligator sinensis. Zoological Research, 2004, 25(5): 415-421.
    [15] TIAN Hong, WEI Rong-ping, ZHANG Gui-quan, SUN Ru-yong, LIU Ding-zhen. Behavioral Difference of Sub-adult Giant Pandas (Ailuropoda melanoleuca) in Traditional and Semi-natural Enclosures. Zoological Research, 2004, 25(2): 137-140.
    [16] CHEN Dong-sheng, NIE Liu-wang, CHENG Shuang-huai, KAN Xian-zhao, WANG Chao-lin, XIE Wan-shu. Amplification and Sequencing of Sox Gene HMG-box in Alligator sinensis. Zoological Research, 2003, 24(2): 127-131.
    [17] WEI Qin, LI Rui, WANG Wan-yu, XIONG Yu-liang. Cloning and Sequence Analysis of cDNAs Encoding Four C-type Lectin-like Proteins from Snake Trimeresurus mucrosquamatus. Zoological Research, 2002, 23(4): 273-279.
    [18] ZHA Hong-Guang, ZHANG Yun. Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus. Zoological Research, 2001, 22(5): 392-396.
    [19] ZHANG Ya-ping, CHEN Xing, SHI Li-ming. Restriction Enzyme Map of mtDNA From Giant Panda (Ailuropoda melanoleuca). Zoological Research, 1991, 12(2): 209-214.
    [20] CHEN Wen-yuan, WANG Xi-zhong, WANG Zi-shu, HE Guang-xin, YE Zhi-yong. Study on the Banded Chromosomes of the Giant Panda (Ailuropoda melanolenca). Zoological Research, 1985, 6(2): 201-207.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2422) PDF downloads(1663) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return