Volume 17 Issue 4
Jul.  1996
Turn off MathJax
Article Contents
WU Chuan-fen, DAI Jia-ling, YANG Xin-lin, LI Jing-yan, WANG Yong-chao. The Detection of Centromere Proteins in Tetrahymena thermophila. Zoological Research, 1996, 17(4): 495-499.
Citation: WU Chuan-fen, DAI Jia-ling, YANG Xin-lin, LI Jing-yan, WANG Yong-chao. The Detection of Centromere Proteins in Tetrahymena thermophila. Zoological Research, 1996, 17(4): 495-499.

The Detection of Centromere Proteins in Tetrahymena thermophila

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1996-11-22
  • Up to now,the studies on the centromere proteins are almost carried out in yeast and higher organisms in the world.In this paper we report our studies on centromere proteins in one kind of ciliates,which is much lower than yeast in the evolutionary position mainly with indirect immunofluorescent microscopy and western immunoblotting techniques using two ACA sera,ra-ACA-2,Maca-2,and mAb37A5 as probes.The control material in the study is human HepⅡ cell.With immunofluorescent stain with Chinese ACA antiserum,in the interphase nucleus of HepⅡ cells only very minute pots-precentromere could be observed.This means that the ACA antiserum can actually and specifically recognize the protein components of centromer/kinetochore.When Tetrahymena,was stained with the same technique,there were only patched within nuclei composed of minute fluorescent spots;no precentromere could be distinguished,although all the nuclei gave positive reaction.After immunobloted with Chinese ACA serum and SH serum,the positive band-pattern (from 14 Kd to 140 Kd) of Tetrahymena was highly similar to the band-pattern of human HepⅡ cells.The blotting results with Maca-2 antibody showed that HepⅡ cells gave two bands (80 Kd,stained deely and 120 Kd,stained slightly),Tetrahymena,gave three positive bands (80 Kd,120 Kd,and 150 Kd).The blotting results with ra-ACA-2 antiserum showed that Hep Ⅱ and Tetrahymena all gave 50 Kd,60 Kd and 80 Kd bands.In the immunoblotting with monoclonal antibody mAb37A5,HepⅡ cells gave two bands very similar to those of CHO cells.Tetrahymena gave only one band with molecular weight somewhat lower than 140 Kd.The results described above indicat the high similarity in the centromere/kinetochore protein components between HepⅡ cells and the Tetrahymena.must have emerged earlier in life evolutionary history.
  • loading
  • Relative Articles

    [1] Si-Wei ZHANG, Jiang-Nan FENG, Yi CAO, Li-Ping MENG, Shu-Lin WANG. Autophagy prevents autophagic cell death in Tetrahymena in response to oxidative stress. Zoological Research, 2015, 36(3): 167-173.
    [2] CHANG Yue, FENG Li-Fang, XIONG Jie, MIAO Wei. Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila. Zoological Research, 2011, 32(5): 476-484.  doi: 10.3724/SP.J.1141.2011.05476
    [3] LU Xing-Yi, XIONG Jie, YUAN Dong-Xia, MIAO Wei. Alternative splicing of an ATP-binding cassette transporter ABCC10 in Tetrahymena thermophila. Zoological Research, 2011, 32(6): 605-610.  doi: 10.3724/SP.J.1141.201106605
    [4] FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276.  doi: 10.3724/SP.J.1141.2011.03267
    [5] GUAN Mo, YANG Shi-hua, SI Wei, CAI Ke-jun, JI Wei-zhi. Effect of Bovine Serum Albumin on Vitrification of Pronuclear-Stage Mouse Embryo. Zoological Research, 2005, 26(2): 197-202.
    [6] DENG Li, ZHANG Wei-min, LIN Hao-ran. Effects of Starvation on the Contents of Growth Hormone and Triiodothyronine in Serum,and Lipid and Protein of White Muscles and Livers in Black Seabreams. Zoological Research, 2003, 24(2): 94-98.
    [7] FEI Li-Song, CHEN Hong-Wei, ZHENG Yu-Cai, WANG Qiang, WANG Jie, LI Guang-Han, ZHANG An-Jiu. Protein Composition of the Giant Panda Milk. Zoological Research, 2001, 22(5): 421-424.
    [8] MIAO Wei, YU Yu-He, SHEN Yun-Fen. Phylogenetic Relationships among Tetrahymena shanghaienisis and two Strains of Tetrahymena thermophila Inferred from ITS-1 Sequences. Zoological Research, 2001, 22(4): 265-269.
    [9] YANG Gui-wen, AN Li-guo WANG Chang-fa, WEN Wu-jun. Comparative Studies on The Immunoglobulin in skin Mucus and Serum of Carp. Zoological Research, 1998, 19(6): 489-492.
    [10] NIU Yang-ping, SHAO Jing-yu. Purification and Characterization of Antitoxic protein in The Serum of Agkistrodon halys Pallas. Zoological Research, 1998, 19(6): 493-494.
    [11] WU Chuan-fen, DAI Jia-ling, LI Jing-yan, WANG Yong-chao. Modifications of The Protein Immunoblotting Techniques. Zoological Research, 1996, 17(3): 315-322.
    [12] WU Chuan-fen, LI Jing-yan, DAI Jia-ling, LU Si-qi, WANG Yong-chao. The Responses of Archezoa and Archaebacteria to The Antibodies Against Mammalian Centromere/Kinetochore Proteins. Zoological Research, 1996, 17(3): 323-330.
    [13] WU Chuan-fen, LI Jing-yan, DAI Jia-ling, WANG Yong-chao. The Detection of Centromere/Kinetochore Proteins in Typical Dinoflagellate and Oxyrrhis. Zoological Research, 1996, 17(3): 307-313.
    [14] WU Chuan-fen, DAI Jia-ling, LI Jing-yan, WANG Yong-chao. The Detection of Centromere Proteins in Guglena gracilis. Zoological Research, 1996, 17(4): 501-507.
    [15] LOU Yun-dong, SHEN Hong, LU Jun, SHI Hong. Comparative Studies on Biochemical Composition in Serum of. Zoological Research, 1991, 12(2): 181-185.
    [16] XU Jin-ju, TANG Cheng-kui, TANG Shzo-zong. A Analysis of Electrophoresis on the Serum Protein of Rhesus Monkey (Presbytis francoisi). Zoological Research, 1991, 12(1): 47-48.
    [17] SHEN Xi-qi, ZHANG Zuo-ren. A Long- Term Maintenance Method For Tetrahymena. Zoological Research, 1985, 6(zk): 105-108.
    [18] ZHONG Jin-yeng. Analysis of Electrophoresis on the Serum Proteins of Rhesus Monkey (Macaca mulatta). Zoological Research, 1982, 3(4): 473-474.
    [19] , , , . Assay of Normal Values of the Serum Lipids in Macaca mulatta. Zoological Research, 1982, 3(2): 209-212.
    [20] JIANG Lun-fu, CHEN Yuan-chi. Preliminary Studies on the Relationship Between the Snake Classification and its Venom and Serum Proteins Ⅰ.Electrophoretic Analysis of the Venoms and Serum Proteins of Snakes. Zoological Research, 1981, 2(zk): 161-163.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1734) PDF downloads(1418) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint