Volume 10 Issue 2
Mar.  1989
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YAN Ju, LIU Shi-hus, ZHANG Xi-yuan, ZHANG Mu-xian. The application of cell synchronization to chromosome banding of fish and The tentative karyotype analysis of Ctenopharyngodon idellus. Zoological Research, 1989, 10(2): 123-128.
Citation: YAN Ju, LIU Shi-hus, ZHANG Xi-yuan, ZHANG Mu-xian. The application of cell synchronization to chromosome banding of fish and The tentative karyotype analysis of Ctenopharyngodon idellus. Zoological Research, 1989, 10(2): 123-128.

The application of cell synchronization to chromosome banding of fish and The tentative karyotype analysis of Ctenopharyngodon idellus

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1989-05-22
  • Combining cell synchronization in vitro and bromodeoxyuridine (BrdU) incorporation in early synthesis phases and late synthesis phase of cells of Ctenopharyngodon idellus,two kinds of chromosome patterns of replication banding,R- and G-bands were gained after chromosome preparation were irradiated in warm CaCI[2] solution by UV and were stained with Giemsa.Some spreads lengthcncd in late-prophase and prometaphase show more numbers of bands and characteristics of higher resolution.Analysis of G-banded karyotype showe that there are four pairs of chromosomes with satellite.A tentative idiogrammatic representation of G-banded karyotype of Gtenopharyngodon idellusis proposed.The significance,techniques and possible meehanism of chromosome banding of fish are discussed.
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