Volume 28 Issue 5
Sep.  2007
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PENG Wu, TANG Ya-nan, HU Cheng-yu. Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp. Zoological Research, 2007, 28(5): 465-469.
Citation: PENG Wu, TANG Ya-nan, HU Cheng-yu. Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp. Zoological Research, 2007, 28(5): 465-469.

Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp

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  • Corresponding author: HU Cheng-yu
  • Received Date: 2007-06-27
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-10-22
  • Abstract: Double-stranded RNA activated protein kinase (PKR) is an important antiviral protein induced by interferon (IFN). PKR was discovered as the enzyme responsible for inhibition of protein synthesis. CaPKR-like is the first fish PKR-like gene isolated from crucian carp. In the present study, the expression of CaPKR-like in the tissues of crucian and grass carp were analyzed by Western blots. In both species, CaPKR-like had a very low level of constitutive expression in un-stimulated tissues, but was up-regulated in all tissues tested in animals stimulated with Poly I:C for seven days. The expression analysis revealed the same characterization of CaPKR-like as mammalian PKR genes.
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