Volume 28 Issue 4
Jul.  2007
Turn off MathJax
Article Contents
YANG Dong-shan, GUO Xu-dong, HAI Tang, DU Chen-guang, ANG Jian-guo, CANG Ming, LIU Dong-jun, LI Xi-he, BOU Shor-. Human Pro-insulin Transgenic Calf Derived from Somatic Cell Nuclear Transfer. Zoological Research, 2007, 28(4): 409-416.
Citation: YANG Dong-shan, GUO Xu-dong, HAI Tang, DU Chen-guang, ANG Jian-guo, CANG Ming, LIU Dong-jun, LI Xi-he, BOU Shor-. Human Pro-insulin Transgenic Calf Derived from Somatic Cell Nuclear Transfer. Zoological Research, 2007, 28(4): 409-416.

Human Pro-insulin Transgenic Calf Derived from Somatic Cell Nuclear Transfer

More Information
  • Corresponding author: BOU Shor-gan
  • Received Date: 2007-01-19
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-08-22
  • The current study was undertaken to evaluate the possibility of producing a human pro-insulin transgenic cow by means of somatic cell nuclear transfer (SCNT). A double selection system, Neomycin resistance (Neor) gene and enhanced green fluorescent protein (EGFP) gene linked through an inner ibosomal entry site (IRES) sequence directed by a Cytomegalovirus(CMV) promoter, was used for enrichment and selection of the transgenic cells and preimplantation embryos. Transgenes were introduced into bovine fetal fibroblast cells (BFF) cultured in vitro through electroporation (900 V/cm, 5 ms). Transgenic bovine fibroblast cells (TBF) were enriched through addition of G418 in culture medium (800 μg/mL). Before being used as a nuclear donor, the TBF cells were either cultured in normal conditions (10% FBS) or treated with serum starvation (0.5% FBS for 2-4 days) followed by 10 hours recovery for G1 phase synchronization. Transgenic cloned embryos were produced through GFPexpressing cell selection and SCNT. The results were the percentage of blastocyst development following SCNT was lower using TBF than BFF cells (23.2% VS 35.2%, P<0.05). No difference in the percentage of cloned blastocysts between the two groups of transgenic nuclear donor of normal and starvation cultures were observed (23.2% VS 18.9%, P>0.05). Two to four GFP-expressing blastocysts were transferred into the uterus of each synchronised recipient. One pregnancy from of seven recipients (21 embryos) was confirmed by rectum palpation 60 days after embryo transfer and one recipient has given birth to a calf at term. PCR and DNA sequencing analysis confirmed that the calf was produced using human proinsulin transgenic animal.
  • loading
  • Relative Articles

    [1] Zi-Qin Jiang, Han-Yu Wu, Jing Tian, Ning Li, Xiao-Xiang Hu. Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens. Zoological Research, 2020, 41(3): 281-291.  doi: 10.24272/j.issn.2095-8137.2020.032
    [2] Lei Shi, Bing Su. A transgenic monkey model for the study of human brain evolution. Zoological Research, 2019, 40(3): 236-238.  doi: 10.24272/j.issn.2095-8137.2019.031
    [3] MA Yu-Zhen, REN Yu, ZHOU Xue-Yuan, LIU Dong-jun, XU Ri-Gan. Production of transgenic embryos through nuclear transfer using ovine fetal fibroblasts transferred with foreign genes. Zoological Research, 2011, 32(6): 617-623.  doi: 10.3724/SP.J.1141.2011.06617
    [4] PENG Kun-Jing, WANG Jin-Huan, SU Wei-Ting, WANG Xi-Cai, YANG Feng-Tang, NIE Wen-Hui, *. Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting. Zoological Research, 2010, 31(2): 113-121.  doi: 10.3724/SP.J.1141.2010.02113
    [5] HE Ying-ying, LIU Shu-bai, QIAN Jin-qiao, LEE Wen-hui, ZHANG Yun. Mechanism of -CAT Cell Nuclear Transportation andSelectively Killing of Tumor cells. Zoological Research, 2008, 29(4): 386-398.  doi: 10.3724/SP.J.1141.2008.04386
    [6] DUAN Biao, SHAO Hua*, WEI Zhu-ying, WANG Feng-wu, BOU Shor-gan. Germ-line Competent Chimeric Mice Derived from Es Cells of EGFP-transgenic Mouse. Zoological Research, 2007, 28(4): 417-422.
    [7] ZHANG Xiu-zhen CAI Ke-jun WANG Shu-fen HU Zhi-xing PEI Yi-jing JI Wei-zhi , *. Preparation of Rabbit Anti-Human PBMC Antiserum and Rabbit Anti-Rhesus Monkey (Macaca mulatta) Spleen Cell Antiserum and Their Application in Isolating Human and Monkey Inner Cell Masses. Zoological Research, 2006, 27(1): 48-53.
    [8] CAO Hong-guo, LIU Jun-ping, ZHANG Yong. Application of Cultural System Improved in Mouse Somatic Cell Nuclear Transfer and Isolation of ES Cells from Reconstructed Embryos. Zoological Research, 2005, 26(2): 209-213.
    [9] JI Jing-juan, GUO Tong-hang, TONG Xian-hong, LUO Li-hua, ZHOU Gui-xiang, FU Ying-yun, LIU Yu-sheng. Experimental Research on the Construction of Cloned Embryos Through Human-rabbit Inter-species Nuclear Transfer. Zoological Research, 2005, 26(4): 416-421.
    [10] ZHANG Xin, KE Xin, MAO Bi-zeng, HE Zu-hua. Effects of Chitanase and Glucanase Transgenic Rice on Three Species of Soil Collembola and One Species of Annelida. Zoological Research, 2004, 25(4): 273-280.
    [11] LI Yi-song, SUI Shu-guang, ZHANG Li, GU Fu-kang. Intermediate Filament-Nuclear Matrix System of Vegetative- and Resting-Cell of Tachysoma pellionella (Ciliophora,Protozoa). Zoological Research, 2004, 25(2): 147-152.
    [12] LIU Tong-ming, YU Xiao-mu, YE Yu-zhen, WANG Zhong-wei, ZHOU Jian-feng, WU Qing-jiang. Studies on Factors Affecting the Development of Embryos from Somatic Cell Nuclear Transplantation in the Fish. Zoological Research, 2002, 23(2): 107-112.
    [13] YANG Zhen-Yun, GU Fu-Kang, NI Bing, JI Ling-Mei. Intermediate-Type Filament-LaminaNuclear Matrix of Vegetative Cell and Resting Cyst in Pseudourostyla cristata. Zoological Research, 2001, 22(1): 83-84.
    [14] LI Guang-Peng, LAN Guo-Cheng, LIU Ying, YU Yuan-Song et al.. Interspecific Nuclear Transfer Between Mouse and Pig. Zoological Research, 2000, 21(5): 416-418.
    [15] GUO Ren, CHEN Shu-fan, LUO Qi-sheng, WANG Qing-ling, YI Hong-kun, ZHAN Qiong-fen. Transgenic Mice as A Model For Neurovirulence Test of Live Poliomyelitis Vaccines. Zoological Research, 1999, 20(4): 241-246.
    [16] ZHANG Chuan-xi, SUN Jian-xin, SHI Hui-juan, CHEN Zhe-yu, WU Xiang-fu. High Expression of EGFP Gene in The Cells of Cabbage Looper,Trichoplusia ni. Zoological Research, 1999, 20(6): 468-470.
    [17] LIN Li-tang, XIA Shi-ling, ZHU Xin-ping. Studies on Nuclear Transplantation of Somatic Cells in Teleost. Zoological Research, 1996, 17(3): 337-340.
    [18] JI Wei-zhi, YANG Shang-chuan, CHEN Jian-chung, ZOU Ru-jin, SHAN En-yuan. In Vitro Fertilization and Embryo Transfer in Non-Human Primates. Zoological Research, 1993, 14(3): 283-292.
    [19] DAI Yong-xiang, GUAN Zhen-de, HU Xiao-yu, WANG Ming-xiou, DONG De-xiang. Comparison on Biologicai Properties Between KMB-17 and MRC-5 Human Diploid Cell Strains. Zoological Research, 1985, 6(4): 313-318.
    [20] CHEN Que, ZHOU Qi-lin, JIA Xian-li ZHENG De-cun. Inhibition of DNA Repair Ability of Human Esophageal Cancer Cell and CHO-K1 Cell by Hyperthermia. Zoological Research, 1984, 5(3): 227-232.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (3185) PDF downloads(2633) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint