Volume 27 Issue 1
Jan.  2006
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ZHU Xiao-chen LIU Hai-jin , * SUN Xiao-wen XUE Ling-ling MAO Lian-ju. Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus). Zoological Research, 2006, 27(1): 63-67.
Citation: ZHU Xiao-chen LIU Hai-jin , * SUN Xiao-wen XUE Ling-ling MAO Lian-ju. Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus). Zoological Research, 2006, 27(1): 63-67.

Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus)

  • Received Date: 2005-11-07
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-02-22
  • Gynogenesis was thought to be a useful method to generate inbred lines in fish. In this study, analysis of 5-8 heterozygous loci in Japanese flounder (Paralichthys olivaceus) was used to measure the increment of homozygosity in meiogynogenetic diploid (Meiotic-A & Meiotic-B) and mitogynogenetic diploid (Mitotic-A). The result showed all homozygosity in Mitotic-G1, while a high percentage of heterozygosity in Meiosis-A and Meiosis-B except Poli11TUF. The rate of heterozygosity in meiogynogenetic progenies at the locus of Poli9TUF, Poli9-8TUF, Poli11TUF, Poli13TUF, Poli23TUF, Poli30TUF, Poli123TUF and Poli130TUF were 1.000 0, 1.000 0, 0.194 4, 0.945 9, 0.861 1, 1.000 0, 0.777 8 and 0.800 0, respectively. The average recombination rate of these eight loci is 0.822 4. The high proportion of heterozygotes for seven loci demonstrates that it is not a practical method for producing homozygous inbred lines in the gynogenetic fish produced by retention the second polar body; and treatments of suppressing the first cell division were more promising for this purpose.
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