Volume 27 Issue 1
Jan.  2006
Turn off MathJax
Article Contents
ZHANG Xiu-zhen CAI Ke-jun WANG Shu-fen HU Zhi-xing PEI Yi-jing JI Wei-zhi , *. Preparation of Rabbit Anti-Human PBMC Antiserum and Rabbit Anti-Rhesus Monkey (Macaca mulatta) Spleen Cell Antiserum and Their Application in Isolating Human and Monkey Inner Cell Masses. Zoological Research, 2006, 27(1): 48-53.
Citation: ZHANG Xiu-zhen CAI Ke-jun WANG Shu-fen HU Zhi-xing PEI Yi-jing JI Wei-zhi , *. Preparation of Rabbit Anti-Human PBMC Antiserum and Rabbit Anti-Rhesus Monkey (Macaca mulatta) Spleen Cell Antiserum and Their Application in Isolating Human and Monkey Inner Cell Masses. Zoological Research, 2006, 27(1): 48-53.

Preparation of Rabbit Anti-Human PBMC Antiserum and Rabbit Anti-Rhesus Monkey (Macaca mulatta) Spleen Cell Antiserum and Their Application in Isolating Human and Monkey Inner Cell Masses

  • Received Date: 2005-10-11
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-02-22
  • Human peripheral blood mononuclear cells (PBMC) were separated from blood by ficoll-hypaque density gradient centrifugation and rhesus monkey spleen cells were isolated from fresh spleens. To isolate inner cell masses (ICMs) by immunosurgery from human or rhesus monkey embryos and establish embryonic stem cell (ES cell) lines, antiserums were prepared by injecting rabbits with human PBMC or rhesus monkey spleen cells, with 4×108 cells every time, once a week and three times in all, respectively. Our results were listed as below: 1) Rabbit anti-human PBMC antiserum has a titer around 1∶320 and rabbit anti-rhesus spleen cell antiserum has a titer around 1∶640; 2) The trophectoderm were lysed by immunosurgery using the rabbit anti-human PBMC or anti-rhesus spleen cell antiserum followed by exposure to guinea pig complement. ICMs were successfully isolated from 15 human blastocysts and 33 monkey blastocysts. These results indicated that our antiserums could be used for collecting human or monkey ICM cells and they would play important roles in isolating new human or rhesus monkey ES cells in the future.
  • loading
  • Relative Articles

    [1] Zhen-Zhen Chen, Yu-Yu Niu. Stem cell therapy for Parkinson’s disease using non-human primate models. Zoological Research, 2019, 40(5): 349-357.  doi: 10.24272/j.issn.2095-8137.2019.053
    [2] Li-Fang JIN, Jin-Song LI. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research, 2016, 37(4): 205-213.  doi: 10.13918/j.issn.2095-8137.2016.4.205
    [3] GUO Li-Yun, WEI Jing-Kuan, YANG Shang-Chuan, WANG Zheng-Bo. Glaucoma model for stem cell transplantation research in New Zealand white rabbits. Zoological Research, 2012, (2): 225-230.  doi: 10.3724/SP.J.1141.2012.02225
    [4] DONG Jin-Run, GUO Li-Yun, QU Jia-Gui, QI Ren-Li, WANG Wen-Chao, XIAO Chun-Jie, WANG Zheng-Bo. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation. Zoological Research, 2012, 33(1): 43-48.  doi: 10.3724/SP.J.1141.2012.01043
    [5] JIN Li-Fang, JI Shao-Hui, YANG Ji-Feng, JI Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells. Zoological Research, 2011, 32(4): 391-395.  doi: 10.3724/SP.J.1141.2011.04391
    [6] GUO Xiang-Yu, JIN Li-Fang, JI Shao-Hui, JI Wei-Zhi. Rhesus monkey (Macaca mulatta) muller cells exhibit retinal stem/progenitor cell features in vitro. Zoological Research, 2011, 32(6): 611-616.  doi: 10.3724/SP.J.1141.2011.06616
    [7] PENG Kun-Jing, WANG Jin-Huan, SU Wei-Ting, WANG Xi-Cai, YANG Feng-Tang, NIE Wen-Hui, *. Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting. Zoological Research, 2010, 31(2): 113-121.  doi: 10.3724/SP.J.1141.2010.02113
    [8] , , , , , , . Tissue Distribution of TRIM5α in Rhesus Monkey and Upregulation in Peripheral Blood Mononuclear Cell by Using Different Stimuli. Zoological Research, 2009, 30(4): 354-360.  doi: 10.3724/SP.J.1141.2009.04354
    [9] JIN Li-fang, JI Shao-hui, GUO Xiang-yu, WANG Xi-hong, JI Wei-zhi. Induction of Rhesus Monkey Embryonic Stem Cells into Hepatocyte-like Cells by a Three-step Method. Zoological Research, 2009, 30(5): 509-514.  doi: 10.3724/SP.J.1141.2009.05509
    [10] CHEN Dong-liang, LI Rong-rong, ZHANG Jing, LU Bin, WEI Qiang, WANG Shu-fen, XIE Yun-h. Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells. Zoological Research, 2009, 30(4): 345-353.  doi: 10.3724/SP.J.1141.2009.04345
    [11] GUO Ji-tong, LI Xue-feng, Shahnaz Fida, GOU Kemian, Nakisa Malakooti, ZHANG Chun-fan. The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras. Zoological Research, 2009, 30(2): 158-164.  doi: 10.3724/SP.J.1141.2009.02158
    [12] ZHANG Jing, WEI Qiang, LU Bin, CHEN Yong-chang, CHEN Hong-wei, LI Rong-rong. Influence of Different FGF2-expressing Feeder Layers on the Self-renewal and Pluripotency of Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2008, 29(4): 405-414.  doi: 10.3724/SP.J.1141.2008.04405
    [13] LI Rong-rong, CHEN Hong-wei, CHEN Dong-liang, WANG Shu-fen, ZHAN jing, CHEN Rui, J. Hepatocyte Growth Factor Promotes the Proliferation of the Neural Progenitors Derived from Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2008, 29(5): 518-528.  doi: 10.3724/SP.J.1141.2008.05518
    [14] LI Bin, CHEN Hong-wei, HU Zhi-xing, TAN Tao, JIN Li-fang, WANG Shu-fen, JI Wei-zhi, *. Differentiation of Human Embryonic Stem Cells to Neural Progenitors. Zoological Research, 2007, 28(3): 311-319.
    [15] LI Xiang-yun, DI Ke-qian, WEI Wei. Effects of Genetic Background on Establishment of Mouse Embryonic Stem Cells. Zoological Research, 2005, 26(3): 250-254.
    [16] XIE Mei-na, ZHANG Cai-qiao, MI Yu-ling, ZENG Wei-dong. Effects of Follicle-stimulating Hormone and Estrogen on Development of Embryonic Chicken Germ Cell in Vitro. Zoological Research, 2004, 25(1): 53-56.
    [17] PEI Yi-jin, JI Wei-zhi. Induced Differentiation and Apoptosis of Rhesus Monkey Embryonic Stem Cells. Zoological Research, 2003, 24(3): 161-167.
    [18] ZHAO Xiao-fan. Purification of A Cysteine Proteinase From Eggs of Silkmoth (Bombyx mori) and Preparation of The Antiserum. Zoological Research, 1993, 14(1): 93-95.
    [19] CHEN Que, ZHOU Qi-lin, JIA Xian-li ZHENG De-cun. Inhibition of DNA Repair Ability of Human Esophageal Cancer Cell and CHO-K1 Cell by Hyperthermia. Zoological Research, 1984, 5(3): 227-232.
    [20] ZHANG Mei-gui, DAI Yong-xiang, DONG De-xiang. Study on Frozen Preservation and Resuscitation and Biological Characteristics on Primary Dispersed Tissue Cell of Rhesus Monkey's Kidney. Zoological Research, 1983, 4(1): 95-101.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2802) PDF downloads(1772) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return