Volume 28 Issue 5
Sep.  2007
Turn off MathJax
Article Contents
DING Fang, ZHOU Hong-lin, LIU Yang, MA Lan, SU Ying, DU Ling. Effects of Glucose on Development of ICR Mouse Embryosin vitro. Zoological Research, 2007, 28(5): 501-506.
Citation: DING Fang, ZHOU Hong-lin, LIU Yang, MA Lan, SU Ying, DU Ling. Effects of Glucose on Development of ICR Mouse Embryosin vitro. Zoological Research, 2007, 28(5): 501-506.

Effects of Glucose on Development of ICR Mouse Embryosin vitro

More Information
  • Corresponding author: ZHOU Hong-lin
  • Received Date: 2007-06-22
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-10-22
  • Abstract: To study the effects of glucose on the development of ICR mouse embryos in vitro. Experiment 1: ICR female mice (6-8 weeks of age) were super-ovulated with i.p. injections of PMSG and hCG, and mated overnight with males. One-cell embryos were collected at 22-26 hrs after hCG and cultured in CZB supplemented with 0, 0.5, 1.0, 3.0, 5.0 or 10 mmol/L glucose respectively. Experiment 2: One-cell embryos were collected from the oviducts of ICR female mice, super-ovulated and cultured in glucose-free CZB. The embryos,respectively at one-cell, two-cell, four-cell or morula stage,were removed from glucose-free CZB medium and placed in CZB medium supplemented with 3.0 mmol/L glucose (optimal concentration) and removed to glucose-free CZB 24 h later (except the morula stage embryo). The embryos in another group were cultured in CZB supplemented with 3.0 mmol/L glucose during the entire culture time. The embryos in the control group were continuously cultured in glucose-free CZB. Embryos were cultured for 120 h at 37℃ under 5% CO2 in sealed culture chambers and observed every 24 h under a Nikon inverted microscope. The culture efficiency was evaluated by determining the proportion of embryos reaching the four-cell (48 h), blastocyst (96 h), or hatched blastocyst (120 h) stage. The total cell numbers were counted in the blastocyst and hatched blastocyst embryos. The experiment results were: 1) There were no significant differences in the rates of four-cell embryos between every glucose-containing group and the glucose-free group. 2)The blastocyst rates of glucose-containing groups were significantly higher than that of the control. 3)The total cell numbers in the concentration group containing 3.0 mmol/L glucose were significantly higher than one another. 4)Addition of glucose from the two-cell to four-cell stage or from the four-cell to morula stage, significantly increased the blastocyst rates compared with the control. In contrast, addition of glucose from one-cell to two-cell, at morula or after morula, did not increase the blastocyst rates compared with the control. It indicates that addition of glucose to CZB does not result in two-cell block of the embryo development, and addition of glucose to CZB in a concentration as high as 10 mmol/L does not inhibit the development of ICR mouse one-cell to hatched blastocyst development. The optimal concentration of glucose added in CZB could be 3.0 mmol/L in the culture of ICR mouse embryos in vitro. Exposure of embryos to glucose, beginning at the two-cell and extending to the four-cell stage or beginning at the four-cell and extending to the morula stage, is necessary for the development of ICR mouse embryos in vitro.
  • loading
  • Relative Articles

    [1] Jing WANG, Guang LI, Guang-Hui QIAN, Jun-Hao HUA, Yi-Quan WANG. Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway. Zoological Research, 2016, 37(3): 136-143.  doi: 10.13918/j.issn.2095-8137.2016.3.136
    [2] Li-Fang JIN, Jin-Song LI. Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Zoological Research, 2016, 37(4): 205-213.  doi: 10.13918/j.issn.2095-8137.2016.4.205
    [3] Hui-Hua LIU, Cai-Fei HUANG, Xin WANG. Acoustic signal characteristic detection by neurons in ventral nucleus of the lateral lemniscus in mice. Zoological Research, 2014, 35(6): 500-509.  doi: 10.13918/j.issn.2095-8137.2014.6.500
    [4] Ru-Jiang LI, Shu-Dong QIU, Hong TIAN, Sheng-Wei ZHOU. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Zoological Research, 2013, 34(3): 238-243.  doi: 10.11813/j.issn.0254-5853.2013.3.0238
    [5] ZHANG Fu-Xiang, YANG Yi-Bin, KE Gui-Bao, CHEN Ying, XU Xin-Mei, TAN Zhou-Ke, RONG Song. Mice cope with parabiosis — Assessment of their physiological changes of life. Zoological Research, 2012, 33(5): 493-497.  doi: 10.3724/SP.J.1141.2012.05493
    [6] ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua. Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 2012, 33(3): 276-282.  doi: 10.3724/SP.J.1141.2012.03276
    [7] CUI Shu-fang, QU Xiu-hua, HAN Shi-zhong, YU Chen-lin, SUN Wei, TANG Qiu. Application of Dual-color Fluorescence Hybridization Chip Technique in the Monitoring of the Genetic Quality of Inbred Mice Strains. Zoological Research, 2008, 29(1): 10-16.
    [8] HE Zhi-xian, GAO Yun-fang, HU Lin-lin. Comparisons on the Proportion of Type Ⅰand Ⅱ Fibers in Soleus Muscle and the Visceral Indexes in Rats and Mice. Zoological Research, 2005, 26(3): 322-327.
    [9] LI Xiang-yun, DI Ke-qian, WEI Wei. Effects of Genetic Background on Establishment of Mouse Embryonic Stem Cells. Zoological Research, 2005, 26(3): 250-254.
    [10] CAO Hong-guo, LIU Jun-ping, ZHANG Yong. Application of Cultural System Improved in Mouse Somatic Cell Nuclear Transfer and Isolation of ES Cells from Reconstructed Embryos. Zoological Research, 2005, 26(2): 209-213.
    [11] GUAN Mo, YANG Shi-hua, SI Wei, CAI Ke-jun, JI Wei-zhi. Effect of Bovine Serum Albumin on Vitrification of Pronuclear-Stage Mouse Embryo. Zoological Research, 2005, 26(2): 197-202.
    [12] TANG Jia, PI Jian-hui, WANG Dan, WU Fei-jian, CHEN Qi-cai. Effect of Weak Noise on the Frequency Tuning of Mouse Inferior Collicular Neurons. Zoological Research, 2004, 25(3): 191-197.
    [13] WANG Chong, ZHANG Shou-Quan, YANG Guan-Fu. Glucose Metabolism During Kunming Mouse Preimplantation Development:Analysis of Gene Transcription in Embryos in Vivo. Zoological Research, 2001, 22(4): 340-344.
    [14] WANG Hong, ZHANG Yuan-Qiang, DING Yu-Qiang. Immunohistochemical Localization of Neurokinin B Receptor (NK3r) in the Mouse Gastrointestinal Tract. Zoological Research, 2001, 22(2): 110-114.
    [15] GAO Fei, MA Jie, FU Guo-Qiang, WEI Peng, FENG Qiang, LIU Yi-Xun. Expression of TGF-β in the Mouse Placenta. Zoological Research, 2001, 22(4): 269-274.
    [16] WANG Hong, JI Wei-Zhi. Comparison of Bovine Embryo Development In vitro in Brl/Mtcm199 and Hecm-6/mTcm199 Culture Media. Zoological Research, 2000, 21(6): 446-451.
    [17] SHEN Qi-zhang. In Low Temperature Keep the Embryo of Protosalanx Hyalocranius. Zoological Research, 1990, 11(3): 258-258.
    [18] WANG Gui-ying, HU Jie-yu, YAN Yu-juan. Action of Cytochalasin D on Cells of Mouse Mammary Adenocarcinoma Cell Line (MA782/5S-8102). Zoological Research, 1986, 7(1): 39-45.
    [19] XU Yi-sheng, PHILIPPE Brulet. Cytoplasmic Distribution of A Mouse Embryonal Carcinoma Cell Specific RNA,MS3 RNA. Zoological Research, 1983, 4(3): 295-300.
    [20] GUO Jian-min, LIU Rui-qing, ZHANG Yao-ping, DING Lin-hua. The chromosomal Study on the Ascites Reticulum-Cell Sarcoma in Mouse. Zoological Research, 1980, 1(4): 487-499.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2010) PDF downloads(2366) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return