Volume 21 Issue 6
Nov.  2000
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WANG Hong, JI Wei-Zhi. Comparison of Bovine Embryo Development In vitro in Brl/Mtcm199 and Hecm-6/mTcm199 Culture Media. Zoological Research, 2000, 21(6): 446-451.
Citation: WANG Hong, JI Wei-Zhi. Comparison of Bovine Embryo Development In vitro in Brl/Mtcm199 and Hecm-6/mTcm199 Culture Media. Zoological Research, 2000, 21(6): 446-451.

Comparison of Bovine Embryo Development In vitro in Brl/Mtcm199 and Hecm-6/mTcm199 Culture Media

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2000-12-22
  • Cumulus oocyte complexs (COC) were recovered from abattoir cow ovaries collected from slaughterhouse.Then,They were matured and fertilized in TCM199 with 10% BCS.Later on,the one cell embryos were cultured in two culture systems:1)modified TCM199 over a buffalo rat liver (BRL) monolayer co-culture;2)Two stage semi-defined culture system [hamster embryo culture medium with 11 amino acids (HECM-6) followed by tissue culture medium 199+10% bovine calf serum].The results showed that HECM-6/mTCM199 system could improved embryo development to the morula or blastocyst stage higher (50%) than that in BRL/mTCM199 system (41%,P<0.01).This results suggested that HECM-6/mTCM199 two-stage semidefined culture system could provide a significant benefit for the early bovine embryo development,which might be related with glucose concentration and amino acids components in the medium.
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