Volume 23 Issue 5
Sep.  2002
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ZHAO Jing-ya, ZHANG Tian-yi, XU Wei-hua, KANG Le. Molecular Cloning of Diapause Hormone Analog in Helicoverpa assulta. Zoological Research, 2002, 23(5): 367-371.
Citation: ZHAO Jing-ya, ZHANG Tian-yi, XU Wei-hua, KANG Le. Molecular Cloning of Diapause Hormone Analog in Helicoverpa assulta. Zoological Research, 2002, 23(5): 367-371.

Molecular Cloning of Diapause Hormone Analog in Helicoverpa assulta

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2002-10-22
  • Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in Bombyx mori.Here,using bioassay,we present evidence that Has-SGNPⅠ,which clearly exhibited diapause egg inducing activity as does Bom-DH,is likely to be DH.We reported the molecular characterization of the Helicoverpa assulta diapause hormone analog by polymerase chain reaction.A 665 bp fragment containing the entire DH-like gene was isolated and characterized.DH-like gene comprised two exons interspersed by a single intron,and the consensus sequence for splicing junction is identified at the exon/intron boundary.The result shows that DH-like gene is localized in the genome of Helicoverpa assulta.
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