Yiwen WANG, Jinqiang YUAN, Xin GAO, Xianyu YANG*. Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium. Zoological Research, 2012, 33(E5-6): 98-103. doi: 10.3724/SP.J.1141.2012.E05-06E98
Citation: Yiwen WANG, Jinqiang YUAN, Xin GAO, Xianyu YANG*. Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium. Zoological Research, 2012, 33(E5-6): 98-103. doi: 10.3724/SP.J.1141.2012.E05-06E98

Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium

doi: 10.3724/SP.J.1141.2012.E05-06E98
Funds:  National Natural Science Foundation of China (31071881); Research Development Foundation of Zhejiang A & F University (2009FK67).
  • Received Date: 2012-07-23
  • Rev Recd Date: 2012-10-10
  • Publish Date: 2012-12-08
  • There are six micronuclear divisions during conjugation of Paramecium caudatum: three prezygotic and three postzygotic divisions. Four haploid nuclei are formed during the first two meiotic prezygotic divisions. Usually only one meiotic product is located in the paroral cone (PC) region at the completion of meiosis, which survives and divides mitotically to complete the third prezygotic division to yield a stationary and a migratory pronucleus. The remaining three located outside of the PC degenerate. The migratory pronuclei are then exchanged between two conjugants and fuse with the stationary pronuclei to form synkarya, which undergo three successive divisions (postzygotic divisions). However, little is known about the surviving mechanism of the PC nuclei. In the current study, stage-specific appearance of cytoplasmic microtubules (cMTs) was indicated during the third prezygotic division by immunofluorescence labeling with anti-alpha tubulin antibodies surrounding the surviving nuclei, including the PC nuclei and the two types of prospective pronuclei. This suggested that cMTs were involved in the formation of a physical barrier, whose function may relate to sequestering and protecting the surviving nuclei from the major cytoplasm, where degeneration of extra-meiotic products occurs, another important nuclear event during the third prezygotic division.
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  • [1]
    Calkins GN, Cull SW. 1907. The conjugation of Paramecium aurelia (caudatum). Arch Protistenk, 10: 375-415.
    [2]
    Gao X, Zhang XJ, Yang XY. 2010. Morphological apoptotic characteristics of the post-meiotic micronuclei in Paramecium caudatum. Europ J Protistol, 46(3): 243-250.
    [3]
    Gao X, Shi XB, Yang XY. 2011a. Dynamic behaviour of stationary pronuclei during their positioning in Paramecium caudatum. Europ J Protistol, 47(3): 235-237.
    [4]
    Gao X, Zhu JJ, Yang XY, Wang YW, Yuan JQ, Song MG. 2011b. Localization of stationary pronuclei during conjugation of Paramecium indicated by immunofluorescence staining. Zool Res, 32(4): 461-464.
    [5]
    Gao X, Yang XY, Zhu JJ, Yuan, JQ, Wang YW, Song MG. 2011c. New details indicated by different stainings during conjugation of ciliated protozoa Paramecium. Zool Res, 32(6): 651-656.
    [6]
    Hiwatashi K. 1968. Determination and inheritance of mating types in Paramecium caudatum. Genetics, 58(3): 373-386.
    [7]
    Ishida M, Nakajima Y, Kurokawa K, Mikami K. 1999. Nuclear behavior and differentiation in Paramecium caudatum analyzed by immunofluorescence with anti-tubulin antibody. Zool Sci, 16(6): 915-926.
    [8]
    Jurand A. 1976. Some ultrastructural features of micronuclei during conjugation and autogamy in Paramecium aurelia. J Gen Microbiol, 94(1): 193-203.
    [9]
    Nakajima Y, Mikami K, Takahashi M. 2001. Role of the cytoplasmic and the intranuclear microtubules on the behavior of pronuclei during conjugation in Paramecium caudatum. Proc JPN Acad Ser B, 77(9): 172-177.
    [10]
    Nakajima Y, Ishida M, Mikami K. 2002. Microtubules mediate germ-nuclear behavior after meiosis in conjugation of Paramecium caudatum. J Eukaryot Microbiol, 49(1): 74-81.
    [11]
    Orias E, Cervantes MD, Hamilton EP. 2011. Tetrahymena thermophila, a unicellular eukaryote with separate germline and somatic genomes. Res Microbiol, 162(6): 578-586.
    [12]
    Orias JD, Hamilton E, Orias E. 1983. A microtubule meshwork associated with gametic pronucleus transfer across a cell-cell junction. Science, 222(4620): 181-184.
    [13]
    Sun XT, Wu JW, Gao X, Yang XY. 2010. To enhance the production of magnetic iron-dextran particles by ultrasonic treatment and its applications in ciliate studies. Chn J Zool, 45(1): 90-93. (in Chinese)
    [14]
    Taka N, Kurokawa K, ArakiT, Mikami K. 2006. Selection of the germinal micronucleus in Paramecium caudatum: nuclear division and nuclear death. J Eukaryot Microbiol, 53(3): 177-184.
    [15]
    Wichterman R. 1986. The Biology of Paramecium [M]. 2nd Ed. New York: Plenum Press.
    [16]
    Yang XY, Takahashi M. 1999. Disturbance of determination of germinal and somatic nuclei by heat shock in Paramecium caudatum. J Eukaryot Microbiol, 46(1): 49-55.
    [17]
    Yang XY, Takahashi M. 2002. Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum. Europ J Protistol, 38(2): 147-153.
    [18]
    Yang XY, Shi XB. 2007. Gametic nuclear exchange during the conjugation of Paramecium polycaryum. JPN J Protozool, 40: 113-121.
    [19]
    Yang XY, Gao X, Shi XL. 2007. Detection of haploid nuclear death in living Paramecium caudatum. JPN J Protozool, 40: 123-130.
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