Volume 33 Issue 6
Nov.  2012
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WANG Cheng-Ye, FENG Ying, CHEN Xiao-Ming. DNA barcoding analysis of processed medicinal insect Catharsius molossus. Zoological Research, 2012, 33(6): 597-602. doi: 10.3724/SP.J.1141.2012.06597
Citation: WANG Cheng-Ye, FENG Ying, CHEN Xiao-Ming. DNA barcoding analysis of processed medicinal insect Catharsius molossus. Zoological Research, 2012, 33(6): 597-602. doi: 10.3724/SP.J.1141.2012.06597

DNA barcoding analysis of processed medicinal insect Catharsius molossus

doi: 10.3724/SP.J.1141.2012.06597
  • Received Date: 2012-07-05
  • Rev Recd Date: 2012-09-10
  • Publish Date: 2012-12-08
  • The identification of medicinal insects is a complex task, especially when they are processed into pieces or powders. This difficulty has potential to create severe complications. For example, inaccurate identification can affect the safety of clinical application for corresponding medicinal insects. A quick and accurate method to identify these kinds of organisms is needed. Here, we amplified and sequenced the mtCOI gene in processed product of Catharsius molossus, including intact individuals and broken individuals, to test the feasibility of DNA barcoding this kind of sample. After comparing results of different DNA extraction methods, we finally succeed in amplifying and sequencing the barcoding segments in these samples. Our method’s barcoding sequences could clearly distinguish between C. molossus and its allied species. The data also indicated that a degree of interfusion of other insects was present in the broken medicinal C. molossus product. These results are quite promising to the establishment of performance criteria for future identification of medicinal C.molossus that will help ensure the safety application of these medicinal insects.
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  • [1]
    Arctander P. 1995. Comparison of a mitochondrial gene and a corresponding nuclear pseudogene[J]. Proc R Soc Lond B, 262(1363): 13-19.
    Chen SL, Yao H, Han JP, Liu C, Song JY, Shi LC, Zhu YJ, Ma XY, Gao T, Pang XH, Luo K, Li Y, Li XW, Jia XC, Lin YL, Leon C. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species [J]. PLoS One, 5(1): e8613.
    Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates [J]. Mol Mar Biol Biotechnol, 3(5): 294-299.
    Hebert PDN, Cywinska A, Ball SL, deWaard JR. 2003a. Biological identifications through DNA barcodes [J]. Proc R Soc Lond B, 270(1512): 313-321.
    Hebert PDN, Ratnasingham S, deWaard JR. 2003b. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species [J]. Proc Biol Sci, 270(Suppl 1): S96-S99.
    Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM. 2004. Identification of Birds through DNA Barcodes [J]. PLoS Biol, 2(10): e312.
    Jiang SJ. 1999. Chinese Medicinal Insects Integration [M]. Beijing: China Forestry Publishing House. [蒋三俊. 1999. 中国药用昆虫集成. 北京: 中国林业出版社.]
    Li HM. 2000. The clinical application of the dung beetle [J]. J Exter Thera Tradit Chn Med, (3): 9. [李浩明. 2000. 蜣螂的临床应用. 中医外治杂志, (3): 9.]
    Lin WH, Li ZZ, Chen ZP, Zhou SY. 2006. Preservation and extraction of genomic DNA from insects [J]. J Guangxi Normal Univ: Nat Sci Ed, 24(2): 86-88. [林万华, 黎宗展, 陈振鹏, 周善义. 2006. 昆虫全基因组DNA的保存及提取. 广西师范大学学报: 自然科学版, 24(2): 86-88.]
    Liu GQ, Ding CY, Zhang KC. 2008. Effects of medicinal ossified Catharsius molossus on in vitro anti-tumor of Ganoderma lucidum fermented products [J]. Mycosystema, 27(6): 964-972. [刘高强, 丁重阳, 章克昌. 2008. 药用昆虫蜣螂对灵芝发酵产物体外抗肿瘤活性的影响. 菌物学报, 27(6): 964-972.]
    Liu GQ, Ding CY, Zhang KC, Wang XL. 2009. Effects of medicinal insect Catharsius molossus on submerged fermentation and in vivo anti-hepatoma activity of Ganoderma lucidum [J]. Chn J Biotechnol, 25(6): 880-886. [刘高强, 丁重阳, 章克昌, 王晓玲. 2009. 药用昆虫蜣螂对灵芝发酵和抗小鼠肝癌活性的影响. 生物工程学报, 25(6): 880-886.]
    Liu LC, Gu GH, Yang GX, Chen HX, Chen JJ, Wang T, Chen MJ, Wang WL, Chen W, Chen HN, Meng JY, Xie JC. 1995. Observation of medicinal insect Catharsius molossus [J]. Entomolog Knowl, 32(4): 214-215. [刘立春, 顾国华, 杨顾新, 陈惠祥, 陈建军, 王涛, 陈马钧,王万里, 陈蔚, 陈惠年, 陈德山, 孟继元, 谢孝宸. 1995. 药用昆虫神农活蜣螂的观察. 昆虫知识, 32(4): 214-215.]
    Liu MZ, Song JY, Luo K, Lin YL, Liu P, Yao H. 2012. Identification of nine common medicinal plants from Artemisia L. by DNA barcoding sequences [J]. Chn Tradit Herb Drugs, 43(7): 1393-1397. [刘美子, 宋经元, 罗焜, 林余霖, 刘萍, 姚辉. 2012. DNA条形码序列对9种蒿属药用植物的鉴定. 中草药, 43(7): 1393-1397.]
    Mo BH, Qu L, Han S, He JW, Zhao M, Zeng XM. 2008. DNA barcoding identification Ⅰ. Research progress and applied perspective of DNA barcoding [J]. Sichuan J Zool, 27(2): 303-306. [莫帮辉, 屈莉, 韩松, 何建伟, 赵明, 曾晓茂. 2008. DNA条形码识别Ⅰ. DNA条形码研究进展及应用前景. 四川动物, 27(2): 303-306.]
    Nanjing University of Traditional Chinese Medicine. 2006. Great Dictionary of traditional Chinese Medicine [M]. Shanghai: Shanghai Science and Technology Press, 2484. [南京中医药大学. 2006. 中药大辞典. 上海: 上海科学技术出版社, 2484.]
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods [J]. Mol Biol Evol, 28(10): 2731-2739.
    Wang X, Huang B. 2006. Advancement of DNA barcoding in animal taxonomy [J]. Biotechnol Bull, (4): 67-72. [王鑫, 黄兵. 2006. DNA条形编码技术在动物分类中的研究进展. 生物技术通报, (4): 67-72.]
    Wei Z. 2008. Application case of dung beetles in Veterinary clinic [J]. Chn J Tradit Vet Sci, (6): 34-34. [魏中. 2008. 蜣螂在兽医临床上的应用案例. 中兽医学杂志, (6): 34-34.]
    Xiao JH, Xiao H, Huang DW. 2004. DNA barcoding: new approach of biological taxonomy [J]. Acta Zool Sin, 50(5): 852-855. [肖金花, 肖晖, 黄大卫. 2004. 生物分类学的新动向——DNA条形编码. 动物学报, 50(5): 852-855.]
    Yao H, Song JY, Liu C, Luo K, Han JP, Li Y, Pang XH, Xu HX, Zhu YJ, Xiao PG, Chen SL. 2010. Use of ITS2 region as the universal DNA barcode for plants and animals [J]. PLoS One, 5(10): e13102.
    Yoo HS, Eah JY, Kim JS, Kim YJ, Min MS, Paek WK, Lee H, Kim CB. 2006. DNA barcoding Korean birds [J]. Mol Cells, 22(3): 323-327.
    Zhang YC, Liu B, Zheng ZM, Li L. 2002. DNA extraction and RAPD-PCR of insect specimens preserved with different meth-ods [J]. Acta Entomol Sin, 45(5): 693-695. [张迎春, 刘波, 郑哲民, 李力. 2002. 不同保藏处理的昆虫标本DNA提取及其随机扩增多态DNA反应. 昆虫学报, 45(5): 693-695.]
    Zhao XM, Zhu M, Yang M, Tao K, Wang JX. 2006. The study of Catharsius molossus L. on experimental prostatic hyperplasia [J]. Pharmacol Clin Chn Mat Med, 22(5): 37-38. [赵兴梅, 朱敏, 杨明, 陶科, 王锦秀. 2006. 蜣螂抗实验性前列腺增生作用研究. 中药药理与临床, 22(5): 37-38.]Zhu YJ, Chen SL, Yao H, Tan R, Song JY, Luo K, Lu J. 2010. DNA barcoding the medicinal plants of the genus Paris [J]. Acta Pharmaceut Sin, 45(3): 376-382. [朱英杰, 陈士林, 姚辉, 谭睿, 宋经元, 罗焜, 鲁静. 2010. 重楼属药用植物DNA条形码鉴定研究. 药学学报, 45(3): 376-382.]
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