Volume 33 Issue 4
Jul.  2012
Turn off MathJax
Article Contents
MIAO Yong-Wang, HA Fu, GAO Hua-Shan, YUAN Feng, LI Da-Lin2, YUAN Yue-Yun. Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens). Zoological Research, 2012, 33(4): 402-408. doi: 10.3724/SP.J.1141.2012.04402
Citation: MIAO Yong-Wang, HA Fu, GAO Hua-Shan, YUAN Feng, LI Da-Lin2, YUAN Yue-Yun. Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens). Zoological Research, 2012, 33(4): 402-408. doi: 10.3724/SP.J.1141.2012.04402

Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens)

doi: 10.3724/SP.J.1141.2012.04402
More Information
  • Author Bio:

    MIAO Yong-Wang,HA Fu

    MIAO Yong-Wang,HA Fu

  • Corresponding author: MIAO Yong-Wang
  • Received Date: 2012-02-16
  • Rev Recd Date: 2012-04-13
  • Publish Date: 2012-08-10
  • To elucidate the genetic characteristics of the bovine Inhibin α subunit (INHA) gene, the polymorphisms in exon 1 of INHA and its bilateral sequences were assayed using PCR with direct sequencing in buffalo, gayal and yak. A comparative analysis was conducted by pooled the results in this study with the published data of INHA on some mammals including some bovine species together. A synonymous substitution c.73C>A was identified in exon 1 of INHA for buffalo, which results in identical encoding product in river and swamp buffalo. In gayal, two non-synonymous but same property substitutions in exon 1 of INHA, viz. c.62 C>T and c.187 G>A, were detected, which lead to p. P21L, p. V63M changes in INHA, respectively. In yak, nucleotide substitution c.62C>T, c.129A>G were found in exon 1 of INHA, the former still causes p. P21L substitution and the latter is synonymous. For the sequence of the 5'-flanking region of INHA examined, no SNPs were found within the species, but a substitution, c. -6T>G, was found. The nucleotide in this site in gayal, yak and cattle was c. -6G, whereas in buffalo it was c. -6T. Meanwhile, a 6-bp deletion, namely c. 262+31_262+36delTCTGAC, was found in the intron of buffalo INHA gene. For this deletion, wild types (+/+) account for main part in river buffalo while mutant types (-/-) are predominant in swamp buffalo. This deletion was not found in gayal, yak and cattle, though these all have another deletion in the intron of INHA, c. 262+78_262+79delTG. The results of sequence alignment showed that the substitutions c. 43A and c. 67G in exon 1 of INHA are specific to buffalo, whereas the substitutions c. 173A and c. 255G are exclusive to gayal, yak and cattle, and c. 24C, c. 47G, c. 174T and c. 206T are specific to goat. Furthermore, there are few differences among gayal, yak and cattle, but there relatively great differences between buffalo, goat and other bovine species regarding the sequences of INHA exon 1.
  • loading
  • Relative Articles

    [1] San-Xu Liu, Wei Hou, Xue-Yan Zhang, Chang-Jun Peng, Bi-Song Yue, Zhen-Xin Fan, Jing Li. Identification and characterization of short tandem repeats in the Tibetan macaque genome based on resequencing data. Zoological Research, 2018, 39(4): 291-300.  doi: 10.24272/j.issn.2095-8137.2018.047
    [2] Li-Li QING, Hui ZHAO, Lin-Lin LIU. Progress on low susceptibility mechanisms of transmissible spongiform encephalopathies. Zoological Research, 2014, 35(5): 436-445.  doi: 10.13918/j.issn.2095-8137.2014.5.436
    [3] YUAN Feng, MIAO Yong-Wang, LI Da-Lin, TANG Shou-Kun, Xü Zheng, HUO Jin-Long, QI Hong. Genetic characteristics on exon 4 of prolactin gene in 12 water buffalo populations. Zoological Research, 2010, 31(6): 575-580.  doi: 10.3724/SP.J.1141.2010.06575
    [4] YUE Gen-Hua , *, Balazs Kovacs, Laszlo Orban, *. A New Problem with Cross-Species Amplification of Microsatellites: Generation of Non-Homologous Products. Zoological Research, 2010, 31(2): 131-140.  doi: 10.3724/SP.J.1141.2010.02131
    [5] ZHANG Shu-chao, YUE Bi-song, ZOU Fang-dong*. Isolation and Characterization of Microsatellite DNA Markers from Forest Musk Deer (Moschus berezovskii)(in English). Zoological Research, 2007, 28(1): 24-27.
    [6] LIN Lu-ping, LIN Qun, WANG Yi-quan. Cloning of Two Toxin Related Genes and Analysis of Their cDNA Polymorphism. Zoological Research, 2007, 28(5): 457-464.
    [7] YIN Xu-wang, NIU Cui-juan*. Polymorphism and Morphotype Transformations in the Rotifer (Brachionus calyciflorus)(in English). Zoological Research, 2007, 28(1): 68-72.
    [8] HE Zhan-xing, HE Xie-chao, LUO Zai-ren, ZHU Fang-xian, SUZUKI tatsuyuki et al.. Experiments on Synchronous Estrus,Superovulation and Embryo Transfer in Buffaloes of Yunnan. Zoological Research, 2005, 26(1): 106-111.
    [9] SHI Xian-wei, WEI Lin, HAN Hong-jin. Bi-PASA Genetic Marker and Polymorphism of the Porcine STCH Gene. Zoological Research, 2005, 26(1): 76-81.
    [10] LIU Jing-xia, ZHOU Li, ZHAO Zhen-shan, GUI Jian-fang. Studies on Microsatellite Markers of Four Artificially Gynogenetic Families in Ornamental Carp. Zoological Research, 2002, 23(2): 97-105.
    [11] PENG Xian-wen, ZHONG Guang-hui, ZHENG Yu-cai, MAO Yong-jiang, WANG Yong, JIANG Ming-feng. Genetic Polymorphism of Epithelial Mucin,MUC1,in Milk of Goats. Zoological Research, 2002, 23(1): 15-18.
    [12] QIU Gao-feng, CHANG Lin-rui, XU Qiao-ting, FANG Xiong-ying, LOU Yun-dong. Intraspecific DNA Sequence Polymorphism in The Mitochondrial 16 S rRNA Gene of The Chinese Shrimp,Penaeus chinensis. Zoological Research, 2000, 21(1): 35-40.
    [13] YANG Zhao-qing, ZUO Yang-xian, CHEN Xin-wen, DING Bo, LUO Jing, ZHANG Ya-ping. 18S rRNA Gene of Sarcocystis hominis Cyst From Water Buffalo and Cattle. Zoological Research, 2000, 21(2): 133-138.
    [14] MENG Shi-jie, LI Xiao-song. Mitochondrial DNA Polymorphism of Six Drosophila melanogaster Populations in Chinese Mainland. Zoological Research, 1998, 19(4): 301-307.
    [15] XIE Li, WEN Shuo-yang, XIE Yi-quan, PENG Tong-xu. Studies on Mitochondrial DNA Comparisons of Drosophila lini Within Different Populations. Zoological Research, 1997, 18(2): 213-219.
    [16] LIU Wan-chen, CUI Jan-hua, WANG Yu-song, MA Ning, LI Yong-jun, SONG Ya-qin. Transferrin Polymorphism in Cashmere Goat. Zoological Research, 1997, 18(2): 212-220.
    [17] JIA Zhen-yu, ZHU Ding-liang, GENG Zhen-cheng, T.Aotsuka. A Study on Polymorphism of Mitochondrial DNA (mtDNA) in Populations of D.virilis Distributing in Chinese Continent. Zoological Research, 1995, 16(1): 65-73.
    [18] ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Restriction Fragment Length Polymorphism in Presbytis phayrei and P.francoisi. Zoological Research, 1993, 14(3): 263-269.
    [19] ZHANG Ya-ping, SHI Li-ming. Mitochondrial DNA Polymorphisms in animals:A Review. Zoological Research, 1992, 13(3): 289-298.
    [20] MA Cai-xia MA Kun SHI Li-ming YU Gui-xin, GONG Shi-he PENG Chang-fen YANG Xiang-ping. Analysis of Synaptonemal Complexes in Spermatocytes of the Hybrid F1 Between Swamp and Murrah Buffalo. Zoological Research, 1991, 12(3): 305-308.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1513) PDF downloads(1944) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint