Volume 33 Issue 4
Jul.  2012
Turn off MathJax
Article Contents
LI Xin-Yi, LIN Yu-Shuang, ZHANG Hong-Wei. Phylogenetic analysis and expression patterns of tropomyosin in amphioxus. Zoological Research, 2012, 33(4): 389-394. doi: 10.3724/SP.J.1141.2012.04389
Citation: LI Xin-Yi, LIN Yu-Shuang, ZHANG Hong-Wei. Phylogenetic analysis and expression patterns of tropomyosin in amphioxus. Zoological Research, 2012, 33(4): 389-394. doi: 10.3724/SP.J.1141.2012.04389

Phylogenetic analysis and expression patterns of tropomyosin in amphioxus

doi: 10.3724/SP.J.1141.2012.04389
More Information
  • Author Bio:

    LI Xin-Yi

  • Corresponding author: LI Xin-Yi
  • Received Date: 2012-01-06
  • Rev Recd Date: 2012-06-13
  • Publish Date: 2012-08-10
  • In amphioxus, we found a mesoderm related gene, tropomyosin,which encodes a protein comprising 284 amino acid residues, sharing high identities with other known Tropomyosin proteins both in vertebrates and invertebrates. Phylogenetically, amphioxus Tropomyosin fell outside the invertebrate clade and was at the base of the vertebrate protein family clade, indicating that it may represent an independent branch. From the early neurula to the larva stage, whole-mount in situ hybridization and histological sections found transcripts of amphioxus tropomyosin gene. Weak tropomyosin expression was first detected in the wall of the archenteron at about 10 hours-post-fertilization neurula stage, while intense expression was revealed in the differentiating presumptive notochord and the muscle. Transcripts of tropomyosin were then expressed in the formed notochord and somites. Gene expression seemed to continue in these developing organs throughout the neurular stages and remained till 72-hours, during the early larval stages. In situ study still showed tropomyosin was also expressed in the neural tube, hepatic diverticulum, notochord and the spaces between myotomes in adult amphioxus. Our results indicated that tropomyosin may play an important role in both embryonic development and adult life.
  • loading
  • Relative Articles

    [1] Xun-He Huang, Gui-Mei Li, Xing Chen, Ya-Jiang Wu, Wei-Na Li, Fu-Sheng Zhong, Wen-Zhi Wang, Zhao-Li Ding. Identification of a novel mtDNA lineage B3 in chicken (Gallus gallus domesticus). Zoological Research, 2017, 38(4): 208-210.  doi: 10.24272/j.issn.2095-8137.2017.039
    [2] Jing WANG, Guang LI, Guang-Hui QIAN, Jun-Hao HUA, Yi-Quan WANG. Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway. Zoological Research, 2016, 37(3): 136-143.  doi: 10.13918/j.issn.2095-8137.2016.3.136
    [3] Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312.  doi: 10.13918/j.issn.2095-8137.2014.4.307
    [4] Wei-Ye LI, Yi-Quan WANG. Establishment of full-sib families of Branchiostoma japonicum and the relationship between early development patterns and larvae survival rates. Zoological Research, 2013, 34(5): 446-452.  doi: 10.11813/j.issn.0254-5853.2013.5.0446
    [5] LIU Chang-Jing, ZHAO Wei, ZHOU Rong, LIU Nai-Fa. Phylogeny and genetic diversity of Phrynocephalus przewalskii. Zoological Research, 2012, (2): 127-132.  doi: 10.3724/SP.J.1141.2012.02127
    [6] XU Wei, Li Wei-Ye, WANG Yi-Quan. Identification and expressional analysis of green fluorescent protein genes in amphioxus. Zoological Research, 2012, 33(3): 304-313.  doi: 10.3724/SP.J.1141.2012.03304
    [7] ZHAN Zu-Bing, ZHANG Yue, ZHAO Ruo-Ping, WANG Wen. Evolutionary fate and expression patterns of chimeric new genes in Drosophila melanogaster. Zoological Research, 2011, 32(6): 585-595.  doi: 10.3724/SP.J.1141.2011.06585
    [8] YUE Ming-Jin, MO Sai-Jun, SONG Ping, GONG Yan-Zhang. Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Zoological Research, 2011, 32(4): 386-390.  doi: 10.3724/SP.J.1141.2011.04386
    [9] CHEN Mei-Zhen, CHEN Jiong*, LU Xin-Jiang, SHI Yu-Hong. Molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (Plecoglossus altivelis). Zoological Research, 2010, 31(6): 595-600.  doi: 10.3724/SP.J.1141.2010.06595
    [10] ZHANG Ying, JIN Shan, ZHAO Qing-Song, WANG Guo-Liang, YU Kai, WANG Chun-Lin. Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 2010, 31(6): 587-594.  doi: 10.3724/SP.J.1141.2010.06587
    [11] ZHONG Hua-Ming, ZHANG Hong-Hai , *, SHA Wei-Lai, ZHANG Cheng-De, CHEN Yu-Cai. Complete Mitochondrial Genome of the Red Fox (Vuples vuples) and Phylogenetic Analysis with Other Canid Species. Zoological Research, 2010, 31(2): 122-130.  doi: 10.3724/SP.J.1141.2010.02122
    [12] Li Wei-Ye, Zhong Jing, Wang Yi-Quan. Analysis of Amphioxus Geographic Populations in the West Pacific Ocean Based on COX Ι Gene. Zoological Research, 2010, 31(4): 375-380.  doi: 10.3724/SP.J.1141.2010.04375
    [13] WEI Yun-hu, ZHANG Yu-jun, CHEN Yuan, MAO Bing-yu. Expansion of the Actin Gene Family in Amphioxus. Zoological Research, 2009, 30(5): 473-479.  doi: 10.3724/SP.J.1141.2009.05473
    [14] ZHANG Yu-jun, MAO Bing-yu. Developmental Expression of an Amphioxus (Branchiostoma belcheri) Gene Encoding a GATA Transcription Factor. Zoological Research, 2009, 30(2): 137-143.  doi: 10.3724/SP.J.1141.2009.02137
    [15] MA Li, ZHAO Shu-hua, KONG Qing-hua, MAO Bing-yu, *. Temporal and Spatial Expression Patterns of Sox1 Gene in Xenopus laevis Embryo. Zoological Research, 2007, 28(4): 403-408.
    [16] WANG Yi-quan , *, ZHANG Qiu-jin, LU Xiao-mei, ZHONG Jing, SUN Yi. Laboratory Culturing and Acquirement of the Second Filial Generation of Amphioxus. Zoological Research, 2006, 27(6): 631-634.
    [17] WANG Yi-quan, FANG Shao-hua. Taxonomic and Molecular Phylogenetic Studies of Amphioxus: A Review and Prospective Evaluation. Zoological Research, 2005, 26(6): 666-672.
    [18] CHEN Jin-ping, YUAN Hong-mei, WANG Bin, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii). Zoological Research, 2004, 25(6): 527-533.
    [19] WENG You-Zhu, HUANG Wei-Quan, FANG Yong-Qiang, YAO Bing, SUN Lan. Study on GnRH Receptor (GnRHR) mRNA In situ Hybridization in The Nervous System,Hatschek's Pit and Gonad of Amphioxus,Branchiostoma belcheri. Zoological Research, 2000, 21(6): 437-441.
    [20] WANG Jin-xing, ZHAO Xiao-fan, GONG Bo. Studies on The Expression of Isozyme Genes in Pupae and Embryogenesis of Antheraea pernyi. Zoological Research, 1996, 17(3): 239-246.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1611) PDF downloads(2163) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return