Volume 33 Issue 1
Jan.  2012
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XIA Hou-Jun, WANG Chun-Yan, ZHANG Hai-Lin, HE Bao-Li, JIAO Jian-Lin, CHEN Ce-Shi. Characterization of spontaneous breast tumor in tree shrews (Tupaia belangeri chinenesis). Zoological Research, 2012, 33(1): 55-59. doi: 10.3724/SP.J.1141.2012.01055
Citation: XIA Hou-Jun, WANG Chun-Yan, ZHANG Hai-Lin, HE Bao-Li, JIAO Jian-Lin, CHEN Ce-Shi. Characterization of spontaneous breast tumor in tree shrews (Tupaia belangeri chinenesis). Zoological Research, 2012, 33(1): 55-59. doi: 10.3724/SP.J.1141.2012.01055

Characterization of spontaneous breast tumor in tree shrews (Tupaia belangeri chinenesis)

doi: 10.3724/SP.J.1141.2012.01055
Funds:  Yunnan Province High-Profile Talent Project (2010CI114); the Chinese Academy of Sciences, Basic Frontier Project (KSCX2-EWJ- 23)
  • Received Date: 2011-12-02
  • Rev Recd Date: 2012-01-05
  • Publish Date: 2012-02-22
  • Breast cancer is a common malignant tumor. It is essential to develop suitable animal models for discovering novel preventive and therapeutic approaches. Tree shrews (Tupaia belangeri chinensis) have a closer evolutionary relationship with humans than do rodents, which have been widely used in laboratory research. Spontaneous breast tumors were identified in tree shrews in 1960s; however, no detailed studies about tree shrew breast tumors have been conducted to date. Here, we characterized a spontaneous breast tumor from tree shrews by Haematoxylin Eosin (H&E) staining. This tumor was identified as a papillary tumor. Immunohistochemical staining (IHC) for progesterone receptor (PR), Ki-67 and cleaved caspase-3 showed that tumor cells were positive for PR, highly proliferative, and less apoptotic compared to normal breast epithelial cells. Thus, the spontaneous tumor of tree shrew is very close to human papillary tumors in terms of morphology and pathology and we concluded that tree shrew may be a suitable animal model for breast cancer research.
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  • [1]
    Brack M. 1988. Malignant Leydig cell tumour in a Tupaia belangeri: case report and literature review of male genital tumours in nonhuman primates
    [J]. Lab Anim, 22(2): 131-134.
    Brack M, Schwartz P, Heinrichs T, Schultz M, Fuchs E. 1996. Tumors of the respiratory tract observed at the German Primate Center, 1978-1994
    [J]. J Med Primatol, 25(6): 424-434.
    Carey LA, Perou CM, Livasy CA, Dressler LG, Cowan D, Conway K, Karaca G, Troester MA, Tse CK, Edmiston S, Deming SL, Geradts J, Cheang MCU, Nielsen TO, Moorman PG, Earp HS, Millikan RC. 2006. Race, breast cancer subtypes, and survival in the Carolina Breast Cancer Study
    [J]. JAMA, 295(21): 2492-2502.
    Cilotti A, Bagnolesi P, Napoli V, Lencioni R, Bartolozzi C. 1991. Solitary intraductal papilloma of the breast. An echographic study of 12 cases
    [J]. Radiol Med, 82(5): 617-620.
    Collaborative Group on Hormonal Factors in Breast Cancer. 2002. Breast cancer and breastfeeding: collaborative reanalysis of individual data from 47 epidemiological studies in 30 countries, including 50302 women with breast cancer and 96973 women without the disease
    [J]. Lancet, 360(9328): 187-195.
    Darai G, Zöller L, Hofmann W, Möller P, Schwaier A, Flügel RM. 1982. Spontaneous malignomas in Tupaia (tree shrew)
    [J]. Amer J of Primtol, 2(2): 177-189.
    Diamond JR, Borges VF, Eckhardt SG, Jimeno A. 2009. BRCA in breast cancer: from risk assessment to therapeutic prediction
    [J]. Drug News Perspect, 22(10): 603-608.
    Elliot OS, Elliot MW, Lisco H. 1966. Breast cancer in a tree shrew (Tupaia glis)
    [J]. Nature, 211(5053): 1105.
    Fan L, Zheng Y, Yu KD, Liu GY, Wu J, Lu JS, Shen KW, Shen ZZ, Shao ZM. 2009. Breast cancer in a transitional society over 18 years: trends and present status in Shanghai, China
    [J]. Breast Cancer Res Treat, 117(2): 409-416.
    Fasanella S, Leonardi E, Cantaloni C, Eccher C, Bazzanella I, Aldovini D, Bragantini E, Morelli L, Cuorvo LV, Ferro A, Gasperetti F, Berlanda G, Dalla Palma P, Barbareschi M. 2011. Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies
    [J]. Diagn Pathol, 6(S1): S1-S7.
    Hofmann W, Möller P, Schwaier A, Flügel RM, Zöller L, Darai G. 1981. Malignant tumours in Tupaia (tree shrew)
    [J]. J Med Primatol, 10(2-3): 155-163.
    Hulka BS, Stark AT. 1995. Breast cancer: cause and prevention
    [J]. Lancet, 346(8979): 883-887.
    Janecka JE, Miller W, Pringle TH, Wiens F, Zitzmann A, Helgen KM, Springer MS, Murphy WJ. 2007. Molecular and genomic data identify the closest living relative of primates
    [J]. Science, 318(5851): 792-794.
    Jemal A, Siegel R, Ward E, Murray T, Xu JQ, Smigal C, Thun MJ. 2006. Cancer statistics
    [J]. CA Cancer J Clin, 56(2): 106-130.
    Lopez F, Belloc F, Lacombe F, Dumain P, Reiffers J, Bernard P, Boisseau MR. 1991. Modalities of synthesis of Ki67 antigen during the stimulation of lymphocytes
    [J]. Cytometry, 12(1): 42-49.
    Pal SK, Lau SK, Kruper L, Nwoye U, Garberoglio C, Gupta RK, Paz B, Vora L, Guzman E, Artinyan A, Somlo G. 2010. Papillary carcinoma of the breast: an overview
    [J]. Breast Cancer Res Treat, 122(3): 637-645.
    Sariego J. 2010. Breast cancer in the young patient
    [J]. Am Surg, 76(12): 1397-1400. 17
    Thomas GA, Leonard RCF. 2009. How age affects the biology of breast cancer
    [J]. Clin Oncol (R Coll Radiol), 21(2): 81-85.
    Wei H, Jiayi F, Qinping Z, Junyi S, Yuan S, Li L, Dongwei S, Liying Q. 2009. Ultrasound-guided vacuum-assisted breast biopsy system for diagnosis and minimally invasive excision of intraductal papilloma without nipple discharge
    [J]. World J Surg, 33(12): 2579-2581.
    Yager JD, Davidson NE. 2006. Estrogen carcinogenesis in breast cancer
    [J]. N Engl J Med, 354(3): 270-282.
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