Volume 33 Issue 1
Jan.  2012
Turn off MathJax
Article Contents
ZHANG Yuan-Xu, PING Shu-Huang, YANG Shi-Hua. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoological Research, 2012, 33(1): 29-36. doi: 10.3724/SP.J.1141.2012.01029
Citation: ZHANG Yuan-Xu, PING Shu-Huang, YANG Shi-Hua. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoological Research, 2012, 33(1): 29-36. doi: 10.3724/SP.J.1141.2012.01029

Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm

doi: 10.3724/SP.J.1141.2012.01029
  • Received Date: 2011-11-01
  • Rev Recd Date: 2011-12-31
  • Publish Date: 2012-02-22
  • The tree shrew (Tupaia belangeri chinensis) is a small non-rodent mammal, which is a relatively new experimental animal in medicine due to its close evolutionary relationship to primates and its rapid propagation. Sperm characteristics and cryopreservation in the tree shrew were the main contents of our spermatological research. Epididymal sperm were surgically harvested from male tree shrews captured from the Kunming area. The rate of testis weight to body weight was (1.05±0.07)%, volume of both testis was (1.12±0.10) mL, total sperm from epididymis and vas deferens were 2.2?8.8×107, and sperm motility and acrosome integrity were (68.8±3.9)% and (90.0±2.1)%, respectively. Sperm ultrastructure of the tree shrew was examined by scanning electron microscopy and transmission electron microscopy. Tree shrew sperm had a round or oval shaped head of approximately 6.65×5.82 μm, and midpiece, principal piece, tail, and total sperm lengths were 13.39, 52.35, 65.74, and 73.05 μm, respectively. The mitochondria in the midpiece consisted of approximately 48 gyres and had a 9+9+2 axonemal pattern. After freezing and thawing, sperm showed partly intact acrosomes and plasma membrane defects, and sperm breakages, twists, and swellings were found. The tree shrew had similar ultrastructure with other mammalians except for the mitochondria number and the sperm size. Ultrastructural alteration is still the main cause resulting in poor sperm after cryopreservation.
  • loading
  • [1]
    Aboagla EME, Terada T. 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa
    [J]. Theriogenology, 62(6): 1160-1172.
    [3]
    Báo SN, Gonçalves Simões D, Lino-Neto J. 2004. Sperm ultrastructure of the bees Exomalopsis (Exomalopsis) auropilosa Spinola 1853 and Paratetrapedia (Lophopedia) sp. Michener & Moure 1957 (Hymenoptera, Apidae, Apinae)
    [J]. J Submicrosc Cytol Pathol, 36(1): 23-28.
    [5]
    Bavister BD, Leibfried ML, Lieberman G. 1983. Development of preimplantation embryos of the golden hamster in a defined culture medium
    [J]. Biol Reprod, 28(1): 235-247.
    [7]
    Bierla JB, Gizejewski Z, Leigh CM, Ekwall H, Söderquist L, Rodriguez-Martinez H, Zalewski K, Breed WG. 2007. Sperm morphology of the Eurasian beaver, Castor fiber: an example of a species of rodent with highly derived and pleiomorphic sperm populations
    [J]. J Morphol, 268(8): 683-689.
    [9]
    Brito LF, Sertich PL, Stull GB, Rives W, Knobbe M. 2010. Sperm ultrastructure, morphometry, and abnormal morphology in American black bears (Ursus americanus)
    [J]. Theriogenology, 74(8):1403-1413.
    [11]
    Collins PM, Tsang WN. 1987. Growth and reproductive development in the male tree shrew (Tupaia belangeri) from birth to sexual maturity
    [J]. Biol Reprod, 37(2): 261-267.
    [13]
    Collins PM, Tsang WN, Lofts B. 1982. Anatomy and function of the reproductive tract in the captive male tree shrew (Tupaia belangeri)
    [J]. Biol Reprod, 26(1): 169-182.
    [15]
    Collins PM, Tsang WN, Urbanski HF. 2007. Endocrine correlates of reproductive development in the male tree-shrew (Tupaia belangeri) and the effects of infantile exposure to exogenous androgens
    [J]. Gen Comp Endocrinol, 154(1-3): 22-30.
    [17]
    Downing Meisner A, Klaus AV, O'Leary MA. 2005. Sperm head morphology in 36 species of artiodactylans, perissodactylans, and cetaceans (Mammalia)
    [J]. J Morphol, 263(2): 179-202.
    [19]
    EI-Shoura SM, Abdel Aziz M, Ali ME, EI-Said MM, Ali KZM, Kemeir MA, Raoof AMS, Allam M, Elmalik EMA. 1995. Deleterious effects of khat addiction on semen parameters and sperm ultrastructure
    [J]. Hum Reprod, 10(9): 2295-2300.
    [21]
    Fischman ML, Bolondi A, Cisale H. 2009. Ultrastructure of sperm 'tail stump' defect in wild boar
    [J]. Andrologia, 41(1): 35-38.
    [23]
    Gage MJG. 1998. Mammalian sperm morphometry
    [J]. Proc Biol Sci, 265(1391): 97-103.
    [25]
    Jones RC, Martin ICA. 1973. The effects of dilution, egg yolk and cooling to 5oC on the ultrastructure of ram spermatozoa
    [J]. J Reprod Fertil, 35(2): 311-320.
    [27]
    Kozicz T, Bordewin LAP, Czéh B, Fuchs E, Roubos EW. 2008. Chronic psychosocial stress affects corticotropin-releasing factor in the paraventricular nucleus and central extended amygdala as well as urocortin 1 in the non-preganglionic Edinger-Westphal nucleus of the tree shrew
    [J]. Psychoneuroendocrinology, 33(6): 741-754.
    [29]
    Li Y, Wan DF, Wei W, Su JJ, Cao J, Qiu XK, Ou C, Ban KC, Yang C, Yue HF. 2008. Candidate genes responsible for human hepatocellular carcinoma identified from differentially expressed genes in hepatocarcinogenesis of the tree shrew (Tupaia belangeri chinesis)
    [J]. Hepatol Res, 38(1): 85-95.
    [31]
    Lima GL, Barros FFPC, Costa LLM, Castelo TS, Fontenele-Neto JD, Silva AR. 2009. Determination of semen characteristics and sperm cell ultrastructure of captive coatis (Nasua nasua) collected by electroejaculation
    [J]. Anim Reprod Sci, 115(1-4): 225-230.
    [33]
    Liu FGR, Miyamoto MM, Freire NP, Ong PQ, Tennant MR, Young TS, Gugel KF. 2001. Molecular and morphological supertrees for eutherian (placental) mammals
    [J]. Science, 291(5509): 1786-1789.
    [35]
    Maeda S, Nakamuta N, Nam SY, Kimura J, Endo H, Rerkamnuaychoke W, Kurohmaru M, Yamada J, Hayashi Y, Nishida T. 1998. Monoclonal antibody TSd-1 is specific to elongating and matured spermatids in testis of common tree shrew (Tupaia glis)
    [J]. J Vet Med Sci, 60(3): 345-349.
    [37]
    Mancini K, Lino-Neto J, Dolder H, Dallai R. 2009. Sperm ultrastructure of the European hornet Vespa crabro (Linnaeus, 1758) (Hymenoptera: Vespidae)
    [J]. Arthropod Struct Dev, 38(1): 54-59.
    [39]
    Mazur P. 1984. Freezing of living cells: mechanisms and implications
    [J]. Am J Physiol, 247(3 Pt 1): C125-C142.
    [41]
    Nagy F. 1994. On the ultrastructure of the spermatozoa in the Siberian hamster (Phodopus sungorus campbelli)
    [J]. J Submicrosc Cytol Pathol, 26(4): 533-544.
    [43]
    O'Meara CM, Hanrahan JP, Donovan A, Fair S, Rizos D, Wade M, Boland MP, Evans ACO, Lonergan P. 2005. Relationship between in vitro fertilisation of ewe oocytes and the fertility of ewes following cervical artificial insemination with frozen-thawed ram semen
    [J]. Theriogenology, 64(8): 1797-1808.
    [45]
    Ortman K, Rodriguez-Martinez H. 1994. Membrane damage during dilution, cooling and freezing-thawing of boar spermatozoa packaged in plastic bags
    [J]. J Vet Med Ser, 41(1-10): 37-47.
    [47]
    Pedersen H. 1969. Ultrastructure of the ejaculated human sperm
    [J]. Z Zellforsch Mikrosk Anat, 94(4): 542-554.
    [49]
    Ping S, Wang F, Zhang Y, Wu C, Tang W, Luo Y, Yang S. 2011. Cryopreservation of epididymal sperm in tree shrews (Tupaia belangeri)
    [J]. Theriogenology, 76(1): 39-46.
    [51]
    Poonkhum R, Pongmayteegul S, Meeratana W, Pradidarcheep W, Thongpila S, Mingsakul T, Somana R. 2000. Cerebral microvascular architecture in the common tree shrew (Tupaia glis) revealed by plastic corrosion casts
    [J]. Microsc Res Tech, 50(5): 411-418.
    [53]
    Poveda A, Kretz R. 2009. c-Fos expression in the visual system of the tree shrew (Tupaia belangeri)
    [J]. J Chem Neuroanat, 37(4): 214-228.
    [55]
    Remple MS, Reed JL, Stepniewska I, Lyon DC, Kaas JH. 2007. The organization of frontoparietal cortex in the tree shrew (Tupaia belangeri): II. Connectional evidence for a frontal-posterior parietal network
    [J]. J Comp Neurol, 501(1): 121-149.
    [57]
    Saragusty J, Gacitua H, Rozenboim I, Arav A. 2009. Protective effects of iodixanol during bovine sperm cryopreservation
    [J]. Theriogenology, 71(9): 1425-1432.
    [59]
    Si W, Zheng P, Tang XH, He XC, Wang H, Bavister BD, Ji WZ. 2000. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization
    [J]. Cryobiology, 41(3): 232-240.
    [61]
    Simeo CG, Kurtz K, Rotllant G, Chiva M, Ribes E. 2010. Sperm ultrastructure of the spider crab Maja brachydactyla (Decapoda: Brachyura)
    [J]. J Morphol, 271(4): 407-417.
    [63]
    Suphamungmee W, Wanichanon C, Vanichviriyakit R, Sobhon P. 2008. Spermiogenesis and chromatin condensation in the common tree shrew, Tupaia glis
    [J]. Cell Tissue Res, 331(3): 687-699.
    [65]
    Watson PF. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function
    [J]. Reprod Fertil Dev, 7(4): 871-891.
    [67]
    White IG. 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review
    [J]. Reprod Fertil Dev, 5(6): 639-658.
    [69]
    Wong PY, Kaas JH. 2009. Architectonic Subdivisions of Neocortex in the tree shrew (Tupaia belangeri)
    [J]. Anat Rec-Adv Int Anat Evol Biol, 292(7): 994-1027.
    [71]
    Xu XP, Chen HB, Cao XM, Ben KL. 2007. Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma
    [J]. J Gen Virol, 88(9): 2504-2512.
    [73]
    Zambello E, Fuchs E, Abumaria N, Rygula R, Domenici E, Caberlotto L. 2010. Chronic psychosocial stress alters NPY system: different effects in rat and tree shrew
    [J]. Prog Neuropsychopharmacol Biol Psychiatry, 34(1): 122-130.
  • Relative Articles

    [1] Rong-Nan Li, Zhen-Dong Zhu, Yi Zheng, Ying-Hua Lv, Xiu-E Tian, De Wu, Yong-Jun Wang, Wen-Xian Zeng. Metformin improves boar sperm quality via 5′-AMP-activated protein kinase-mediated energy metabolism in vitro. Zoological Research, 2020, 41(5): 527-538.  doi: 10.24272/j.issn.2095-8137.2020.074
    [2] Jian-Ping LI, Yun LIAO, Ying ZHANG, Jing-Jing WANG, Li-Chun WANG, Kai FENG, Qi-Han LI, Long-Ding LIU. Experimental infection of tree shrews(Tupaia belangeri) with Coxsackie virus A16. Zoological Research, 2014, 35(6): 485-491.  doi: 10.13918/j.issn.2095-8137.2014.6.485
    [3] Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499.  doi: 10.13918/j.issn.2095-8137.2014.6.492
    [4] Xiao-Yun WU, Yun-Hai LI, Qing CHANG, Lin-Qiang ZHANG, Sha-Sha LIAO, Bin LIANG. Streptozotocin induction of type 2 diabetes in tree shrew. Zoological Research, 2013, 34(2): 108-115.  doi: 10.3724/SP.J.1141.2013.02108
    [5] LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6.  doi: 10.3724/SP.J.1141.2012.01001
    [6] SUN Yong-Mei, YANG Jian-Zhen, SUN Hua-Ying, MA Yuan-Ye, WANG Jian-Hong. Establishment of tree shrew chronic morphine dependent model. Zoological Research, 2012, 33(1): 14-18.  doi: 10.3724/SP.J.1141.2012.01014
    [7] WANG Xiao-Ai, YANG Jun-Xing, CHEN Xiao-Yong, PAN Xiao-Fu, LI Zai-Yun. Cryopreservation of sperm from Neolissochilus benasi. Zoological Research, 2012, 33(3): 283-289.  doi: 10.3724/SP.J.1141.2012.03283
    [8] PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 2012, 33(1): 19-28.  doi: 10.3724/SP.J.1141.2012.01019
    [9] WANG Jing, ZHOU Qi-Xin, TIAN Men, YANG Yue-Xiong, XU Lin. Tree shrew models: A chronic social defeat model of depression and a one-trial captive conditioning model of learning and memory. Zoological Research, 2011, 32(1): 24-30.  doi: 10.3724/SP.J.1141.2011.01024
    [10] MA Xu-Tong, LI Fu-Lin, JIANG Hong-Jun, LI Wen-Hui, ZHANG Yun, DU Ting-Yi. Detection and comparison of physiological indexes in the wild and laboratory tree shrew. Zoological Research, 2011, 32(1): 4-10.  doi: 10.3724/SP.J.1141.2011.01004
    [11] YANG Chun, ZHANG Yu-hui, *. Expression of c-fos, Oestradiol and Oestrogen Receptor in the Testis of Rana chensinensis at Different Stages. Zoological Research, 2007, 28(3): 303-310.
    [12] WANG Hui-chun, TAI Fa-dao, LIAN Yi. Age-specific Changes in Estrogen Receptors α in Testis and Epididymis of Mandarin Voles (Microtus mandarinus). Zoological Research, 2005, 26(4): 435-441.
    [13] ZHANG Li, BEN Kun-Long. In vitro Infection of Tree Shrew Immunocytes with Human Immunodeficiency Virus Type Ⅰ. Zoological Research, 2001, 22(1): 33-40.
    [14] ZHU Dong-Fa, WANG Gui-Zhong, LI Shao-Jing. Sperm Ultrastructure of The Squat Lobster,Thenus orientalis (Crustacea,Decapoda). Zoological Research, 2000, 21(5): 387-391.
    [15] LIU Hong-lin, JI Qing, CHENG Yi-feng, JIANG Zhi-hua, HUANG Yue-ying. Detecting Association of Exogenous DNA With Chicken Sperm Using Autoradiography. Zoological Research, 1997, 18(3): 332-340.
    [16] YE Zhi-zhang, PENG Yen-zhang, PAN Ru-liang, WANG Hong. Arterial System in Chinese Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(2): 131-138.
    [17] CAO Xiao-mei. Microscopic Structure of Ovary and Ovarian Activity of Different Seasons in Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(1): 17-24.
    [18] MA Cai-xia, MA Kun, SHI Li-ming. Electron Microscopic Observations on The Synaptonemal Complex in Spermatocytes of The Tree Shrew (Tupaiabelangeri Chinensis). Zoological Research, 1989, 10(zk): 29-33.
    [19] ZHANG Ya-ping, ZHANG Bing, SHI Li-ming. Restriction Maps of Mitochondrial DNA of Slow Loris and Tree Shrew. Zoological Research, 1989, 10(zk): 79-89.
    [20] CAO Xiao-mei. Seasonal Changes in Spermatogenesis of Tree Shrew (Tupaia Belangeri Chinensis). Zoological Research, 1989, 10(1): 15-21.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1563) PDF downloads(1999) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return