Volume 33 Issue 1
Jan.  2012
Turn off MathJax
Article Contents
PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 2012, 33(1): 19-28. doi: 10.3724/SP.J.1141.2012.01019
Citation: PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 2012, 33(1): 19-28. doi: 10.3724/SP.J.1141.2012.01019

Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)

doi: 10.3724/SP.J.1141.2012.01019
  • Received Date: 2011-11-01
  • Rev Recd Date: 2011-12-28
  • Publish Date: 2012-02-22
  • The tree shrew may be an important experimental animal for disease models in humans. The effects of some extenders and momamines on sperm cryopreservation will provide helpful data for experimentation of strains and conservation of genetic resources in tree shrews. Epididymal sperm were surgically harvested from male tree shrews captured around Kunming, China and sperm motility, acrosome integrity and fertility were assessed during cryopreservation. In Experiment 1 eight extenders (TTE, TCG, TCF, TTG, BWW, BTS, DM, and SR) supplemented with 0.4 mol/L DMSO were used to dilute the sperm: only TTE, DM and SR showed no differences in motility and acrosome integrity compared to fresh controls after equilibration. After freezing and thawing, sperm in any extender showed lower motility than fresh control and sperm in DM showed higher motility than other groups. However, BWW produced the lowest motility. For acrosome intergrity, TTE and DM showed higher than BWW, BTS and SR after equilibration. The parameter in DM was higher than other groups (except TTE) after thawing. In Experiment 2 four penetrating cryoprotectant agents (CPA) [dimethyl-formamide (DF), formamide (F), dimethylacetamide (DA), and acetamide (A)] at 0.2 mol/L, 0.4 mol/L, 0.8 mol/L, and 1.2 mol/L, respectively were added to the DM extender. Motility showed no difference among CPA groups and non-CPA group (control) after equilibration, but all thawed sperm showed lower values in motility and acrosome intergrity than pre-freezing groups. However, sperm in 0.8 mol/L DF and 0.4 mol/L DMSO showed higher values in both parameters than that in other CPA groups (P>0.05). In Experiment 3 the fertilization rate of oocytes inseminated with 0.4mol/L DMSO (50%) were higher than that with 0.8mol/L DF (16%). In conclusion, non-ion extenders supplemented with egg yolk may be better for sperm cryopreservation in tree shrews and cryoprotectant effects of monoamines agents should be further studied in this species.
  • loading
  • [1]
    Anel L, Kaabi M, Abroug B, Alvarez M, Anel E, Boixo JC, de la Fuente LF, de Paz P. 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay
    [J]. Theriogenology, 63(4): 1235-1247.
    Bathgate R, Maxwell WM, Evans G. 2006. Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality
    [J]. Reprod Domest Anim, 41(1): 68-73.
    Bavister BD, Leibfried ML, Lieberman G. 1983. Development of preimplantation embryos of the golden hamster in a defined culture medium
    [J]. Biol Reprod, 28(1): 235-247.
    Bianchi I, Calderam K, Maschio EF, Madeira EM, da Rosa Ulguim R, Corcini CD, Bongalhardo DC, Correa EK, Lucia T Jr, Deschamps JC, Correa MN. 2008. Evaluation of amides and centrifugation temperature in boar semen cryopreservation
    [J]. Theriogenology, 69(5): 632-638.
    Cao XM, Ben KL, Wang XL. 2001. Ovulation in the tree shrew (Tupaia belangeri) induced by gonadotrophins
    [J]. Reprod Fertil Dev, 13(5-6): 377-382.
    Chen S, Xu L, Lü LB, Yao YG. 2011. Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China
    [J]. Zool Res (in Chinese), 32(1): 17-23.
    Collins PM, Tsang WN, Urbanski HF. 2007. Endocrine correlates of reproductive development in the male tree-shrew (Tupaia belangeri) and the effects of infantile exposure to exogenous androgens
    [J]. Gen Comp Endocrinol 154(1-3): 22-30.
    Coscioni AC, Reichenbach HD, Schwartz J, LaFalci VS, Rodrigues JL, Brandelli A. 2001. Sperm function and production of bovine embryos in vitro after swim-up with different calcium and caffeine concentration
    [J]. Anim Reprod Sci, 67(1-2): 59-67.
    Coulter GH, Foote RH. 1975. Lipid deficient extender for bovine spermatozoa: its development and use in measuring freezing-induced lipid loss
    [J]. J Dairy Sci 58(1): 82-87.
    De Graaf SP, Evans G, Maxwell WM, Cran DG, O'Brien JK. 2007. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa
    [J]. Theriogenology, 67(2): 391-398.
    Dube C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL. 2004. Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation
    [J]. Theriogenology, 62(5): 874-886.
    Farshad A, Khalili B, Fazeli P. 2009. The effect of different concentrations of glycerol and DMSO on viability of Markhoz goat spermatozoa during different freezing temperatures steps
    [J]. Pak J Biol Sci, 12(3): 239-245.
    Fernandez-Santos MR, Esteso MC, Montoro V, Soler AJ, Garde JJ. 2006. Influence of various permeating cryoprotectants on freezability of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: effects of concentration and temperature of addition
    [J]. J Androl, 27(6): 734-745.
    Fernandez-Santos MR, Esteso MC, Soler AJ, Montoro V, Garde JJ. 2005. The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa
    [J]. Cryo Letters, 26(1): 25-32.
    Futino DO, Mendes MC, Matos WN, Mondadori RG, Lucci CM. 2010. Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation
    [J]. Reprod Domest Anim, 45(2): 214-220.
    Gabriel Sanchez-Partida L, Maginnis G, Dominko T, Martinovich C, McVay B, Fanton J, Schatten G. 2000. Live rhesus offspring by artificial insemination using fresh sperm and cryopreserved sperm
    [J]. Biol Reprod, 63(4): 1092-1097.
    Garde JJ, del Olmo A, SolerAJ, Espeso G, Gomendio M, Roldan ER. 2008. Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri)
    [J]. Anim Reprod Sci, 108(3-4): 384-401.
    Juliani GC, Henry M. 2008. Effects of glycerol, ethylene glycol, acetamide, and dried skim milk in cryopreservation of equine sperm
    [J]. Arq Bras Med Vet Zoo, 60(5): 1103-1109.
    Kaneko T, Yamamura A, Ide Y, Ogi M, Yanagita T, Nakagata N. 2006. Long-term cryopreservation of mouse sperm
    [J]. Theriogenology, 66(5):1098-1101.
    Kashiwazaki N, Okuda Y, Seita Y, Hisamatsu S, Sonoki S, Shino M, Masaoka T, Inomata T. 2006. Comparison of glycerol, lactamide, acetamide and dimethylsulfoxide as cryoprotectants of Japanese white rabbit spermatozoa
    [J]. J Reprod Dev, 52(4): 511-516.
    Kozicz T, Bordewin LA, Czeh B, Fuchs E, Roubos EW. 2008. Chronic psychosocial stress affects corticotropin-releasing factor in the paraventricular nucleus and central extended amygdala as well as urocortin 1 in the non-preganglionic Edinger-Westphal nucleus of the tree shrew
    [J]. Psychoneuroendocrinology, 33(6): 741-754.
    Li Y, Wan DF, Wei W, Su JJ, Cao J, Qiu XK, Ou C, Ban KC, Yang C, Yue HF. 2008. Candidate genes responsible for human hepatocellular carcinoma identified from differentially expressed genes in hepatocarcinogenesis of the tree shrew (Tupaia belangeri chinesis)
    [J]. HepatolRes,38(1): 85-95.
    Li YH, Cai KJ, Kovacs A, Ji WZ. 2005. Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa
    [J]. J Androl,26(3): 387-395.
    Li YH, Cai KJ, Li J, Dinnyes A, Ji WZ. 2006. Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)
    [J]. Am J Primatol, 68(1): 39-49.
    Li YH, Cai KJ, Su L, Guan M He XC, Wang H, Kovacs A, Ji WZ 2005. Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender
    [J]. Asian J Androl, 7(2): 139-144.
    Martins SG, Miranda PV, Brandelli A. 2003. Acrosome reaction inhibitor released during in vitro sperm capacitation
    [J]. Int J Androl, 26(5): 296-304.
    Moce E, Vicente JS, Lavara R. 2003. Effect of freezing-thawing protocols on the performance of semen from three rabbit lines after artificial insemination
    [J]. Theriogenology, 60(1): 115-123.
    Moustacas V, Cruz B, Varago F, Miranda D, Lage P, Henry M. 2011. Extenders containing dimethylformamide associated or not with glycerol are ineffective for ovine sperm cryopreservation
    [J]. Reprod Domest Anim, 46(5): 924-925
    O'Meara CM, Hanrahan JP, Donovan A, Fair S, Rizos D, Wade M, Boland MP, Evans AC, P Lonergan. 2005. Relationship between in vitro fertilisation of ewe oocytes and the fertility of ewes following cervical artificial insemination with frozen-thawed ram semen
    [J]. Theriogenology, 64(8): 1797-1808.
    Paudel KP, Kumar S, Meu SK, Kumaresan A. 2010. Ascorbic acid, catalase and chlorpromazine reduce cryopreservation-induced damages to crossbred bull spermatozoaa
    [J]. Reprod Domest Anim, 45(2): 256-262.
    Ping S, Wang F, Zhang Y, Wu C, Tang W, Luo Y, Yang S. 2011. Cryopreservation of epididymal sperm in tree shrews (Tupaia belangeri)
    [J]. Theriogenology, 76(1): 39-46.
    Poonkhum R, Pongmayteegul S, Meeratana W, Pradidarcheep W, Thongpila S, Mingsakul T, Somana R. 2000. Cerebral microvascular architecture in the common tree shrew (Tupaia glis) revealed by plastic corrosion casts
    [J]. Microsc Res Tech, 50(5): 411-418.
    Poveda A, Kretz R. 2009. c-Fos expression in the visual system of the tree shrew (Tupaia belangeri)
    [J]. J Chem Neuroanat, 37(4): 214-228.
    Rasul Z, Ahmad N, Anzar M. 2001. Changes in motion characteristics, plasma membrane integrity, and acrosome morphology during cryopreservation of buffalo spermatozoa
    [J]. J Androl, 22(2): 278-283.
    Remple MS, Reed JL, Stepniewska I, Lyon DC, Kaas JH. 2007. The organization of frontoparietal cortex in the tree shrew (Tupaia belangeri): II. Connectional evidence for a frontal-posterior parietal network
    [J]. J Comp Neurol, 501(1): 121-149.
    Santiago-Moreno J, Coloma MA, Dorado J, Pulido-Pastor A, Gomez-Guillamon F, Salas-Vega R, Gomez-Brunet A, Lopez-Sebastian A. 2009. Cryopreservation of Spanish ibex (Capra pyrenaica) sperm obtained by electroejaculation outside the rutting season
    [J]. Theriogenology, 71(8): 1253-1260.
    Santiago-Moreno J, Coloma MA, Toledano-Diaz A, Gomez-Brunet A, Pulido-Pastor A, Zamora-Soria A, Carrizosa JA, Urrutia B, Lopez-Sebastian A. 2008. A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa
    [J]. Cryobiology, 57(1): 25-29.
    Santiago-Moreno J, Toledano-Diaz A, Pulido-Pastor A, Dorado J, Gomez-Brunet A, Lopez-Sebastian A. 2006. Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa
    [J]. Theriogenology, 66(5): 1219-1226.
    Saragusty J, Gacitua H, Rozenboim I, Arav A. 2009. Protective effects of iodixanol during bovine sperm cryopreservation
    [J]. Theriogenology, 71(9): 1425-1432.
    Si W, Zheng P, Li YH, Dinnyes A, Ji WZ. 2004. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm
    [J]. Am J Primatol, 62(4): 301-306.
    Si W, Zheng P, Tang X, He X, Wang H, Bavister BD, Ji W. 2000. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization
    [J]. Cryobiology, 41(3): 232-240.
    Sztein JM, Farley JS, Mobraaten LE. 2000. In vitro fertilization with cryopreserved inbred mouse sperm
    [J]. Biol Reprod, 63(6): 1774-1780.
    Tateno H, Mikamo K. 1987. A chromosomal method to distinguish between X- and Y-bearing spermatozoa of the bull in zona-free hamster ova
    [J]. J Reprod Fertil, 81(1): 119-125.
    Underwood SL, Bathgate R, Maxwell WM, Evans G. 2010. Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm
    [J]. Anim Reprod Sci, 118(2-4): 171-175.
    Xu XP, Chen HB, Cao XM, Ben KL. 2007. Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma
    [J]. J Gen Virol 88: 2504-2512.
    Yeste M, Briz M, Pinart E, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Bussalleu E, Casas I, Bonet S.2008. Hyaluronic acid delays boar sperm capacitation after 3 days of storage at 15 degrees C
    [J]. Anim Reprod Sci, 109(1-4): 236-250.
    Yildiz C, Kaya A, Aksoy M, Tekeli T. 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing
    [J]. Theriogenology,54(4): 579-585.
    Zambello E, Fuchs E, Abumaria N, Rygula R, Domenici E, Caberlotto L. 2010. Chronic psychosocial stress alters NPY system: different effects in rat and tree shrew
    [J]. Prog Neuropsychopharmacol Biol Psychiatry, 34(1): 122-130.
  • Relative Articles

    [1] Shu Wei, Hai-Rong Hua, Qian-Quan Chen, Ying Zhang, Fei Chen, Shu-Qing Li, Fan Li, Jia-Li Li. Dynamic changes in DNA demethylation in the tree shrew (Tupaia belangeri chinensis) brain during postnatal development and aging. Zoological Research, 2017, 38(2): 96-102.  doi: 10.24272/j.issn.2095-8137.2017.013
    [2] Jian-Ping LI, Yun LIAO, Ying ZHANG, Jing-Jing WANG, Li-Chun WANG, Kai FENG, Qi-Han LI, Long-Ding LIU. Experimental infection of tree shrews(Tupaia belangeri) with Coxsackie virus A16. Zoological Research, 2014, 35(6): 485-491.  doi: 10.13918/j.issn.2095-8137.2014.6.485
    [3] Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499.  doi: 10.13918/j.issn.2095-8137.2014.6.492
    [4] Ling XU, Yu FAN, Xue-Long JIANG, Yong-Gang YAO. Molecular evidence on the phylogenetic position of tree shrews. Zoological Research, 2013, 34(2): 70-76.  doi: 10.3724/SP.J.1141.2013.02070
    [5] Xiao-Yun WU, Yun-Hai LI, Qing CHANG, Lin-Qiang ZHANG, Sha-Sha LIAO, Bin LIANG. Streptozotocin induction of type 2 diabetes in tree shrew. Zoological Research, 2013, 34(2): 108-115.  doi: 10.3724/SP.J.1141.2013.02108
    [6] LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6.  doi: 10.3724/SP.J.1141.2012.01001
    [7] SUN Yong-Mei, YANG Jian-Zhen, SUN Hua-Ying, MA Yuan-Ye, WANG Jian-Hong. Establishment of tree shrew chronic morphine dependent model. Zoological Research, 2012, 33(1): 14-18.  doi: 10.3724/SP.J.1141.2012.01014
    [8] ZHANG Yuan-Xu, PING Shu-Huang, YANG Shi-Hua. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoological Research, 2012, 33(1): 29-36.  doi: 10.3724/SP.J.1141.2012.01029
    [9] WANG Jing, ZHOU Qi-Xin, TIAN Men, YANG Yue-Xiong, XU Lin. Tree shrew models: A chronic social defeat model of depression and a one-trial captive conditioning model of learning and memory. Zoological Research, 2011, 32(1): 24-30.  doi: 10.3724/SP.J.1141.2011.01024
    [10] MA Xu-Tong, LI Fu-Lin, JIANG Hong-Jun, LI Wen-Hui, ZHANG Yun, DU Ting-Yi. Detection and comparison of physiological indexes in the wild and laboratory tree shrew. Zoological Research, 2011, 32(1): 4-10.  doi: 10.3724/SP.J.1141.2011.01004
    [11] ZHANG Ding, GAO Li, ZHANG Yuan-xu, SUN Li, FENG Yue, HE You-wen, XIA Xue-shan, ZHAN. Crucial Factors for de novo Establishment of Long-term Primary Culture of Tree Shrew Hepatocytes. Zoological Research, 2009, 30(1): 24-30.  doi: 10.3724/SP.J.1141.2009.01024
    [12] YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.
    [13] ZHANG Li, BEN Kun-Long. In vitro Infection of Tree Shrew Immunocytes with Human Immunodeficiency Virus Type Ⅰ. Zoological Research, 2001, 22(1): 33-40.
    [14] ZHENG Zi-xiu, ZHONG Jin-yan. Studies on The Lactate Dehydrogenase Isoenzymes of Tree Shrew (Tupaia belangeri chinensis) Tissues:An Electrophoretic Analysis on The Agarose Gel ). Zoological Research, 1991, 12(1): 85-91.
    [15] CAO Xiao-mei. Microscopic Structure of Ovary and Ovarian Activity of Different Seasons in Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(1): 17-24.
    [16] YE Zhi-zhang, PENG Yen-zhang, PAN Ru-liang, WANG Hong. Arterial System in Chinese Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(2): 131-138.
    [17] ZOU Ru-jin, TIAN Bao-pin, JI Wei-zhi, LI Rui-yuan. Research on The Piebaldism of Tree Shrews Breeding in The Captivity. Zoological Research, 1989, 10(zk): 68-78.
    [18] CAO Xiao-mei. Seasonal Changes in Spermatogenesis of Tree Shrew (Tupaia Belangeri Chinensis). Zoological Research, 1989, 10(1): 15-21.
    [19] MA Cai-xia, MA Kun, SHI Li-ming. Electron Microscopic Observations on The Synaptonemal Complex in Spermatocytes of The Tree Shrew (Tupaiabelangeri Chinensis). Zoological Research, 1989, 10(zk): 29-33.
    [20] ZHANG Ya-ping, ZHANG Bing, SHI Li-ming. Restriction Maps of Mitochondrial DNA of Slow Loris and Tree Shrew. Zoological Research, 1989, 10(zk): 79-89.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1966) PDF downloads(1929) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint