Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents

LI Sheng-An; LEE Wen-Hui; ZHANG Yun

doi:10.3724/SP.J.1141.2012.01001

Volume 33 Issue 1

Jan. 2012

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LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6. doi: 10.3724/SP.J.1141.2012.01001

Citation:

LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6. doi: 10.3724/SP.J.1141.2012.01001

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Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents

doi: 10.3724/SP.J.1141.2012.01001

1.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223, China;
2.
Graduate School of the Chinese Academy of Science, Beijing 100049, China

Funds: This study was financially supported by the Project from the Chinese Academy of Sciences (KSCX2-EW-R-11), the Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province (KSCX2-EW-J-23), and Science and Technology Department of Yunnan Province (2011C1139)

Received Date: 2011-10-17
Rev Recd Date: 2012-01-10
Publish Date: 2012-02-22

Abstract

Abstract

Animal models are essential for the development of new anti-infectious drugs. Although some bacterial infection models have been established in rodents, small primate models are rare. Here, we report on two bacterial infection models established in tree shrew (Tupaia belangeri chinensis). A burnt skin infection model was induced by dropping 5×10⁶ CFU of Staphylococcus aureus on the surface of a wound after a third degree burn. This dose of S. aureus caused persistent infection for 7 days and obvious inflammatory response was observed 4 days after inoculation. A Dacron graft infection model, 2×10⁶ CFU of Pseudomonas aeruginosa also caused persistent infection for 6 days, with large amounts of pus observed 3 days after inoculation. These models were used to evaluate the efficacy of levofloxacin (LEV) and cefoperazone (CPZ), which reduced the viable bacteria in skin to 4log₁₀ and 5log₁₀ CFU/100 mg tissue, respectively. The number of bacteria in graft was significantly reduced by 4log₁₀ CFU/mL treatment compared to the untreated group (P<0.05). These results suggest that two bacterial infection models were successfully established in tree shrew using P. aeruginosa and S. aureus. In addition, tree shrew was susceptible to P. aeruginosa and S. aureus, thus making it an ideal bacterial infection animal model for the evaluation of new antimicrobials.
- Tree shrew,
- Novel antimicrobials,
- Graft infection,
- Staphylococcus aureus,
- Pseudomonas aeruginosa

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References(44)

References

[1]	Cao J, Yang EB, Su JJ, Li Y, Chow P. 2003. The tree shrews: adjuncts and alternatives to primates as models for biomedical research
[J]. J Med Primatol, 32(3): 123-130.
[3]	Craig W. 1993. Relevance of animal models for clinical treatment
[J]. Eur J Clin Microbiol Infect Dis, 12(Suppl 1): S55-S57.
[5]	Dale RMK, Schnell G, Wong JP. 2004. Therapeutic efficacy of "nubiotics" against burn wound infection by Pseudomonas aeruginosa
[J]. Antimicrob Agents Chemother, 48(8): 2918-2923.
[7]	Druilhe P, Hagan P, Rook GAW. 2002. The importance of models of infection in the study of disease resistance
[J]. Trends Microbiol, 10(10): S38-S46.
[9]	Fischbach MA, Walsh CT. 2009. Antibiotics for emerging pathogens
[J]. Science, 325(5944): 1089-1093.
[11]	Giacometti A, Cirioni O, Gov Y, Ghiselli R, Del Prete MS, Mocchegiani F, Saba V, Orlando F, Scalise G, Balaban N, Dell'Acqua G. 2003. RNA III inhibiting peptide inhibits in vivo biofilm formation by drug-resistant Staphylococcus aureus
[J]. Antimicrob Agents Chemother, 47(6): 1979-1983.
[13]	Givskov M, Hentzer M. 2003. Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections
[J]. J Clin Invest, 112(9): 1300-1307.
[15]	McCormick C, Caballero A, Tang AH, Balzli C, Song J, O'Callaghan R. 2008. Effectiveness of a new tobramycin (0.3%) and dexamethasone (0.05%) formulation in the treatment of experimental Pseudomonas keratitis
[J]. Curr Med Res Opin, 24(6): 1569-1575.
[17]	McPhee JB, Hancock REW. 2005. Function and therapeutic potential of host defence peptides
[J]. J Pept Sci, 11(11): 677-687.
[19]	Muller S, Stanyon R, O'Brien PC, Ferguson-Smith MA, Plesker R, Wienberg J. 1999. Defining the ancestral karyotype of all primates by multidirectional chromosome painting between tree shrews, lemurs and humans
[J]. Chromosoma, 108(6): 393-400.
[21]	Niewiesk S, Prince G. 2002. Diversifying animal models: the use of hispid cotton rats (Sigmodon hispidus) in infectious diseases
[J]. Lab Anim, 36(4): 357-372.
[23]	Oyston PCF, Fox MA, Richards SJ, Clark GC. 2009. Novel peptide therapeutics for treatment of infections
[J]. J Med Microbiol, 58(8): 977-987.
[25]	Rafla K, Tredget EE. 2011. Infection control in the burn unit
[J]. Burns, 37(1): 5-15.
[27]	Retsema JA, Bergeron JM, Girard D, Milisen WB, Girard AE. 1993. Preferential concentration of azithromycin in an infected mouse thigh model
[J]. J Antimicrob Chemother, 31(Suppl E): 5-16.
[29]	Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA. 2008. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology
[J]. Infect Immun, 76(8): 3577-3586.
[31]	Tredget EE, Shankowsky HA, Rennie R, Burrell RE, Logsetty S. 2004. Pseudomonas infections in the thermally injured patient
[J]. Burns, 30(1): 3-26.
[33]	Turgut H, Sacar S, Kaleli I, Sacar M, Goksin I, Toprak S, Asan A, Cevahir N, Tekin K, Baltalarli A. 2005. Systemic and local antibiotic prophylaxis in the prevention of Staphylococcus epidermidis graft infection
[J]. BMC Infect Dis, 5: 91.
[35]	Wada T, Kida T, Inoue T, Tokushige H, Naka H, Sakaki H. 2008. Immunomodulatory effect of gatifloxacin on mouse peritoneal macrophages in vitro and in models of endotoxin-induced rat conjunctivitis and rabbit bacterial keratitis
[J]. Ophthalmic Res, 40(2): 54-60.
[37]	Yang EB, Cao J, Su JJ, Li Y, Chow P. 2003. The tree shrews: adjuncts and alternatives to primates as models for biomedical research
[J]. J Med Primatol, 32(3): 123-130.
[39]	Zasloff M. 2002. Antimicrobial peptides of multicellular organisms
[J]. Nature, 415(6870): 389-395.
[41]	Zhang Y, Zhao H, Yu GY, Liu XD, Shen JH, Lee WH, Zhang Y. 2010. Structure-function relationship of king cobra cathelicidin
[J]. Peptides, 31(8): 1488-1493.
[43]	Zhao XP, Tang ZY, Klumpp B, Wolff-Vorbeck G, Barth H, Levy S, von Weizsäcker F, Blum HE, Baumert TF. 2002. Primary hepatocytes of Tupaia belangeri as a potential model for hepatitis C virus infection
[J]. J Clin Invest, 109(2): 221-232.