Volume 31 Issue 4
Jul.  2010
Turn off MathJax
Article Contents
LU Xiang-Yun, ZHANG Ying, WANG Xing-Guo, ZHANG Yan-Ping, XU Jian-Rong, LAI Ren. Identification and Characterization of Myostatin Gene in Rough-skinned Sculp, Trachidermus fasciatus. Zoological Research, 2010, 31(4): 387-394. doi: 10.3724/SP.J.1141.2010.04387
Citation: LU Xiang-Yun, ZHANG Ying, WANG Xing-Guo, ZHANG Yan-Ping, XU Jian-Rong, LAI Ren. Identification and Characterization of Myostatin Gene in Rough-skinned Sculp, Trachidermus fasciatus. Zoological Research, 2010, 31(4): 387-394. doi: 10.3724/SP.J.1141.2010.04387

Identification and Characterization of Myostatin Gene in Rough-skinned Sculp, Trachidermus fasciatus

doi: 10.3724/SP.J.1141.2010.04387
  • Received Date: 2009-12-08
  • Rev Recd Date: 2010-07-13
  • Publish Date: 2010-08-22
  • Myostatin is a member of the TGF-β superfamily and acts as a negative regulator of skeletal muscle growth. The characterization of the myostatin gene and its expression in Trachidermus fasciatus was reported in the current study. A full-length of 2 568 bp myostatin cDNA sequence in T. fasciats was cloned by 5' and 3' RACE, which included a 1 128 bp complete ORF encoding a 375 amino acid peptide, a 106 bp long 5'-UTR and a 1134 bp long 3′UTR. As other MSTN, the putative peptide contains a 22 amino acids long signal peptide, a conserved RXXR proteolytic processing site, and 10 conserved cysteine residues in the C terminal of the protein. The Trachidermus fasciatus MSTN has high homology with Umbrina cirrosa, Morone saxatilis, Morone americana, Morone chrysops myostatin while has low homology with mammalian and birds myostatin. The phylogenetic analysis showed that the T. fasciatus myostatin had the closest relationship with U. cirrosa. In the four examined tissues, the myostatin gene was highly expressed in muscle and intestine and weakly expressed in brain and liver. These results suggested that the fish myostatin gene might not only play roles in muscle development but also contribute to other biological functions.
  • loading
  • [1]
    Garikipati DK, Gahr SA, Rodgers BD. 2006. Identification, characterization, and quantitative expression analysis of rainbow trout myostatin-1a and myostatin-1b genes [J]. J Endocrin, 190: 879-888.Garikipati DK, Gahr SA, Roalson EH, Rodgers BD. 2007. Characterization of rainbow trout myostatin-2 genes (rtMSTN-2a and -2b): Genomic organization, differential expression, and pseudogenization [J]. Endocrinology, 148(5): 2106-2115.Gu Z, Zhang Y, Shi P, Zhang YP, Zhu D, Li H. 2004a. Comparison of avian myostatin genes [J]. Anim Genet, 35(6): 470-472.Gu Z, Zhu D, Li N, Li H, Deng X, Wu C. 2004b.The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth [J]. Sci Chn Ser CLife Sci, 47(1): 26-32.Ji S, Losinski RL, Cornelius SG, Frank GR, Willis GM, Gerrard DE, Depreux FF, Spurlock ME. 1998. Myostatin expression in porcine tissues: Tissue specificity and developmental and postnatal regulation [J]. Am J Physiol, 275 (4 Pt 2): 1265-1273.Lee SJ, McPherron AC. 2001. Regulation of myostatin activity and muscle growth [J]. Proc Natl Acad Sci USA, 98(16): 9306-9311.Maccatrozzo L, Bargelloni L, Cardazzo B, Rizzo G, Patarnello T. 2001. A novel second myostatin gene is present in teleost fish [J]. FEBS Lett, 509(1): 36-40.Mcpherron AC, Lawler AM, Lee SJ. 1997. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily membe [J]. Nature, 387: 83-90.Østbye TK, Galloway TF, Nielsen C, Gabestad I, Bardal T, Andersen Ø. 2001. The two myostatin genes of Atlantic salmon (Salmo salar) are expressed in a variety of tissues[J]. Eur J Biochem, 268(20): 5249-5257.Rescan PY, Jutel I, Ralliere C. 2001. Two myostatin genes are differentially expressed in myotomal muscles of the trout (Oncorhynchus mykiss) [J]. J Exp Biol, 04(Pt 20): 3523-3529.Roberts SB, Goetz FW. 2001. Differential skeletal muscle expression of myostatin across teleost species, and the isolation of multiple myostatin isoforms [J]. FEBS Lett, 491(3): 212-216.Rodgers BD, Weber GM. 2001a. Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana [J]. Comp Biochem Physiol B Biochem Mol Biol, 129 (2-3): 597-603.Rodgers BD, Weber GM, Sullivan CV, Levine MA. 2001b. Isolation and characterization of myostatin complementary deoxyribonucleic acid clones from two commercially important fish: Oreochromis mossambicus and Morone chrysops [J]. Endocrinology, 142(4): 1412-1418.Rodgers BD, Garikipati DK. 2008. Clinical, agricultural, and evolutionary biology of myostatin: A comparative review [J]. Endocr Rev, 29(5): 513-534.Sharma M, Kambadur R, Matthews KG, Somers WG, Devlin GP, Conaglen JV, Fowke PJ, Bass JJ. 1999. Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct [J]. J Cell Physiol, 180 (1): 1-9.Szabo G, Dallmann G, Muller G, Patthy L, Soller M, Varga L. 1998. A deletion in the myostatin gene causes the compact (Cmpt) hypermuscular mutation in mice [J]. Mamm Genome, 9(8): 671-672.Wang JQ, Pan LD, Liang TH, Gan HC. 2004. Preliminary study on embryonic development of the roughskin sculpin, Trachidermus fasciatus王金秋, 潘连德, 梁天红, 甘华臣. 2004. 松江鲈鱼(Trachidermus fasciatus胚胎发育的初步观察. 复旦大学学报: 自然科学版, 43(2): 250-254.]) (Scorpaeniformes: Cottidae) [J]. J Fudan Univ: Nat Sci, 43(2): 250-254. [Westhusin M. 1997. From mighty mice to mighty cows [J]. Nat Genet, 17(1): 4-5.Xie Y, Xue X, Yin H, Tang R, Su Jing, Song K, Hu YT, Wamg H, Yang H. 2008. Homology modeling and molecular evolution analysis of myostatin [J]. Zool Res, 29(5): 485-492. [谢玉为, 薛小平, 尹焕才, 唐蕊华, 苏  婧, 宋  凯, 呼延霆, 汪  桦, 杨  慧. 2008. Myostatin三维结构模建及分子进化分析. 动物学研究, 29(5): 485-492.]Xu JR, Han XL, Yu JF, Bao F, XU P. 2009. Analysis of genetic diversity of Trachidermus fasciatus徐建荣, 韩晓磊, 郁建锋, 鲍  峰, 许  璞. 2009. 松江鲈遗传多样性的ISSR分析. 淡水渔业, 39(1): 21-24, 35.] by ISSR [J]. Freshwat Fisher, 39(1): 21-24, 35. [
    [2]

       
  • Relative Articles

    [1] Liang ZHAO, Xingtao ZHANG, Xingkui TAO, Weiwei WANG, Ming LI. Preliminary analysis of the mitochondrial genome evolutionary pattern in primates. Zoological Research, 2012, 33(E3-4): 47-56.  doi: 10.3724/SP.J.1141.2012.E03-04E47
    [2] XU Wei, Li Wei-Ye, WANG Yi-Quan. Identification and expressional analysis of green fluorescent protein genes in amphioxus. Zoological Research, 2012, 33(3): 304-313.  doi: 10.3724/SP.J.1141.2012.03304
    [3] ZENG Zhen, LIU Zhi-Zhi, PAN Lian-De, TANG Wen-Qiao, WANG Qian, GENG Yun-Hao. Analysis of genetic diversity in wild populations of Trachidermus fasciatus by RAPD and the transformation of two SCAR markers. Zoological Research, 2012, (2): 203-210.  doi: 10.3724/SP.J.1141.2012.02203
    [4] FAN Wu-Jiang, LI Si-Fa. cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron. Zoological Research, 2010, 31(6): 601-609.  doi: 10.3724/SP.J.1141.2010.06601
    [5] ZHANG Min, ZHAO Jin-Liang*, DENG Yan-Fei. Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish,Siniperca chuatsi. Zoological Research, 2010, 31(1): 77-83.  doi: 10.3724/SP.J.1141. 2010.01077
    [6] ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482.  doi: 10.3724/SP.J.1141.2010.05476
    [7] SUN Bao-fa, WANG Rui-wu, HU Zhong. Ovipositing Pattern of the Fig Wasps and Its Effect on the Offspring Sex Ratio. Zoological Research, 2009, 30(5): 559-564.  doi: 10.3724/SP.J.1141.2009.05559
    [8] XIE Yu-wei, XUE Xiao-ping, YIN Huan-cai, TANG Rui-hua, SU Jing, SONG Kai, HUYAN Ting, WANG Hua, YANG Hui. Homology Modeling and Molecular Evolution Analysis of Myostatin. Zoological Research, 2008, 29(5): 485-492.  doi: 10.3724/SP.J.1141.200805485
    [9] LIAO Wan-qin, LIANG Xu-fang *, WANG Lin, MA Xu, FANG Ling, LI Gui-sheng. cDNA Sequence Cloning and Tissue Expression of Uncoupling Protein 2 of Silver Carp (Hypophthalmichthys molitrix). Zoological Research, 2006, 27(4): 375-381.
    [10] LIU Xu-sheng, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli. Zoological Research, 2004, 25(2): 158-161.
    [11] ZHAO Ping, YUAN Xiao, TANG Si-xian, WANG Tian-hou. Species and Habitat Preference of Waterbirds at the Eastern End of Chongming Island (Shanghai) in Winter. Zoological Research, 2003, 24(5): 387-391.
    [12] LI Chang-jian, LIU Jun, SHI Yao-hua, YANG Shu-ting, GUI Jian-fang. Full Length cDNA Cloning and Tissue Expression Specificity of Ran Gene in Color Crucian Carp. Zoological Research, 2003, 24(3): 173-179.
    [13] ZHA Hong-Guang, ZHANG Yun. Purification of a Phospholipase A[2] from Bungarus fasciatus Venom by One Step Sepharose 4B Method. Zoological Research, 2001, 22(6): 433-436.
    [14] ZHA Hong-Guang, ZHANG Yun. Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus. Zoological Research, 2001, 22(5): 392-396.
    [15] LONG Yong-chen, YANG Da-rong et al., SI na-ji-ding, LU Zi et al.. Studies on the Pattern of Spatial Distribution of the Ghost Moths' Larvae. Zoological Research, 1987, 8(1): 47-54.
    [16] . The Determination of pgs Content in Plasma and Tissue of Normal Rabbit. Zoological Research, 1985, 6(4): 419-420.
    [17] , , . Karyotype of Trachidermus Fasciatus Heckel. Zoological Research, 1984, 5(1): 103-104.
    [18] ZHANG Yao-shi, WU Shin-lu, CHEN Yuan-chung, CHEN Shi-qiu. The Amino Acid Composition and Partial Sequence of Cytotoxin ⅩⅢ From Canton Bungarus fasciatus Venom. Zoological Research, 1981, 2(zk): 27-32.
    [19] SUN Yi-an, CHEN Shi-qiu, WU Shin-lu. Physiological Actions of Bungarus fasciatus Postsynaptic Neurotoxin-Xiv. Zoological Research, 1981, 2(zk): 147-148.
    [20] XIAO Chang-hua, ZHAN Hong-ji, LEI Ke-jian. Study on Venom of Bungarus fasciatus,The Purification and Biochemical Analysis of Cardiotoxic Principles. Zoological Research, 1981, 2(1): 49-54.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1995) PDF downloads(2575) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return