Volume 31 Issue 4
Jul.  2010
Turn off MathJax
Article Contents
Li Wei-Ye, Zhong Jing, Wang Yi-Quan. Analysis of Amphioxus Geographic Populations in the West Pacific Ocean Based on COX Ι Gene. Zoological Research, 2010, 31(4): 375-380. doi: 10.3724/SP.J.1141.2010.04375
Citation: Li Wei-Ye, Zhong Jing, Wang Yi-Quan. Analysis of Amphioxus Geographic Populations in the West Pacific Ocean Based on COX Ι Gene. Zoological Research, 2010, 31(4): 375-380. doi: 10.3724/SP.J.1141.2010.04375

Analysis of Amphioxus Geographic Populations in the West Pacific Ocean Based on COX Ι Gene

doi: 10.3724/SP.J.1141.2010.04375
More Information
  • Author Bio:

    Li Wei-Ye

  • Corresponding author: Wang Yi-Quan
  • Received Date: 2009-12-14
  • Rev Recd Date: 2010-04-23
  • Publish Date: 2010-08-22
  • Amphioxus (lancelet) is an important model animal in the field of Evo-Devo study. However, the specimens for laboratorial experiments are usually sampled directly from the field. Thus, it is necessary to correctly identify species and evaluate the genetic diversity of geographic populations. In the present investigation, we amplified and sequenced COX Ι gene of amphioxus collected from various localities of the West Pacific Ocean and downloaded all available sequences of the gene from NCBI. Based on those sequences data, we constructed NJ tree to decide the taxonomic status of those amphioxus and performed AMOVA analysis to estimate genetic diversities among geographic populations and individuals. The current results supported the validity of the three amphioxus species, Branchiostoma malayanum, B. belcheri and B. japonicum. We also proposed that some amphioxus species names recorded in NCBI sequence database might be inaccurate. Genetic analysis on the geographic populations of amphioxus species suggested that each examined amphioxus species have a high level of genetic diversity. However, the genetic divergences among geographic populations of the same species are indetectable, indicating a frequent gene flow among them.
  • loading
  • [1]
     Atarhouch T, Ruber L, Gonzalez EG. 2006. Signature of an early genetic bottleneck in a population of Moroccan sardines (Sardina pilchardus)[J]. Mol Phylogenet Evol, 39(2): 373-383.Cao YP, Yan LN, Xie S, Liu Z. 2001. Preliminary investigation of amphioxus in Changli[J]. Chn J Zool曹玉萍,闫路娜,谢 松,刘 震. 2001. 昌黎海区文昌鱼初步调查. 动物学杂志, 63(3): 10-13.], 63(3): 10-13. [Chen Y, Cheung SG, Kong RYC, Shin PKS. 2007. Morphological and molecular comparisons of dominant amphioxus populations in the China Seas[J]. Mar Biol, 153: 189-198.Flook PK, Klee S, Rowell CHF. 1999. Combined molecular analysis of the Orthoptera and implications for their higher systematics[J]. Syst Biol, 48(2): 233-253.Han Z, Gao T, Yanagimoto T. 2008. Deep phylogeographic break among white croaker Pennahia argentata (Sciaenidae, Perciformes) populations in North-western Pacific[J]. Fish Sci, 74: 770-780.Holland LZ, Holland ND. 2001. Evolution of neural crest and placodes: amphioxus as a model for the ancestral vertebrate?[J]. J Anat, 199: 85-98.Holland LZ, Laudet V, Schubert M. 2004. The chordate amphioxus: an energing model organism for developmental biology[J]. Cell Mol Life Sci, 61: 2290-2308.Hebert PDN, Cywinska A, Ball SL. 2003. Biological identification through DNA barcodes[J]. Proc Roy Soc BBiol Sci, 270: 313-321.Hebert PDN, Ratnasingham S, deWaard JR. 2003. Barcoding animal life: cytochrome coxidase subunit 1 divergences among closely related species[J]. Proc Roy Soc BBiol Sci, 270: 96-99.Kon T, Nohara M, Nishida M, Sterrer W, Nishikawa T. 2006. Hidden ancient diversification in the circumtropical lancelet Asymmetron lucayanum complex[J]. Mar Biol, 149: 875-883.Kumar S, Dudley J, Nei M, Tamura K. 2008. MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences[J]. Brief Bioinform, 9: 299-306.Liu JX, Gao TX, Zhuang ZM. 2006. Late Pleistocene divergence and subsequent population expansion of two closely related fish species, Japanese anchovy (Engraulis japonicus) and Australian anchovy (Engraulis australis)[J]. Mol Phylogenet Evol, 40(3): 712-723.Nishikawa T. 1981. Consideration on the taxonomic status of the lancelets of the genus Branchiostoma from the Japanese waters[J]. Publ Seto Mar Biol Lab, 26: 135-156.Peng JL, Wang XZ, He SP. 2008. The progress and application of DNA barcoding[J]. Acta Hydrobiol Sin2(06) [彭居俐, 王绪桢, 何舜平. 2008. DNA条形码技术的研究进展及其应用. 水生生物学报, 32(06): 916-919.]: 916-919., 3Saitou N, Nei M. 1987. The neighbor-joining method for reconstructing phylogenetic trees[J]. Mol Biol Evol, 4: 406-425.Shu DG, Morris SC, Zhang XL. 1996. A Pikaia-like chordate from the Lower Cambrian of China[J]. Nature, 384: 157-158.Strokes MD, Holland ND. 1998. The lancelet: Also known as "amphioxus", this curious creature has returned to the limelight as a player in the phylogenetic history of the vertebrates[J]. Am Sci, 86: 552-560.Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0[J]. Mol Biol Evol, 24: 1596-1599.Tchang S, Koo KC. 1936. Description of a new variety of Branchiostoma belcheri Gray from Kiaochow Bay, Shantung, China[J]. Contrib Inst Zool Nat Acad Peiping, 3(4): 77-114.Wang YQ, Xu QS, Peng XX, Zhou HT. 2004. Taxonomic status of amphioxus Branchiostoma belcheri in Xiamen beach estimated by homologous sequence of Cyt b gene[J]. Acta Zool Sin王义权, 许群山, 彭宣宪, 周涵韬. 2004. 通过Cyt b基因同源序列比较评估厦门文昌鱼的分类学地位. 动物学报50(2): 202-208.], , 50(2): 202-208. [Wang YQ, Fang SH. 2005. Taxonomic and molecular phylogenetic studies of amphioxus: a review and prospective evaluation[J]. Zool Res, 26(6): 666-672. [王义权, 方少华. 2005. 文昌鱼分类学研究及展望. 动物学研究, 26(6): 666-672.]Xu QS, Ma F, Wang YQ. 2005. Morphological and 12S rRNA gene comparison of two Branchiostoma species in Xiamen waters[J]. J Exp Zool Part B: Mol Dev Evol, 304: 259-267.Zhong J, Zhang QJ, Xu QS, Schubert M, Laudet V, Wang YQ. 2009. Complete mitochondrial genomes defining two distinct lancelet species in the West Pacific Ocean[J]. Mar Biol Res, 5: 278-285.Zhang QJ, Zhong J, Fang SH and Wang YQ. 2006. Branchiostoma japonicum and B. belcheri are distinct lancelets (Cephalochordata) in Xiamen waters in China[J]. Zool Sci, 23: 573-579.Zhang QJ, Li G, Sun Y, Wang YQ. 2009. Chromosome preparation and preliminary observation of two amphioxus species in Xiamen[J]. Zool Res, 30(2): 131-136.Zhu X, Xia L, Song S, Yu XZ, Zhai K. 2003. Branchiostoma belcheri found in the coastal waters of Weihai[J]. Mar Sci祝 茜,夏 龙,宋 帅,于希忠,翟 奎. 2003. 威海海域发现文昌鱼. 海洋科学, 27(9): 6-7.], 27(9): 6-7. [
    [2]

        
  • Relative Articles

    [1] Jing WANG, Guang LI, Guang-Hui QIAN, Jun-Hao HUA, Yi-Quan WANG. Expression analysis of eight amphioxus genes involved in the Wnt/β-catenin signaling pathway. Zoological Research, 2016, 37(3): 136-143.  doi: 10.13918/j.issn.2095-8137.2016.3.136
    [2] Huan WU, Yong-Han HE, Tian-Rui XU, Qing-Peng KONG. Absence of mutation in miR-34a gene in a Chinese longevity population. Zoological Research, 2015, 36(2): 112-114.
    [3] Wei-Ye LI, Yi-Quan WANG. Establishment of full-sib families of Branchiostoma japonicum and the relationship between early development patterns and larvae survival rates. Zoological Research, 2013, 34(5): 446-452.  doi: 10.11813/j.issn.0254-5853.2013.5.0446
    [4] Song LI, Fa-Hong YU. Differentiation in cranial variables among six species of Hylopetes (Sciurinae:Pteromyini). Zoological Research, 2013, 34(E5): 531120.  doi: 10.11813/j.issn.0254-5853.2013.E4-5.E120
    [5] CAI Bo, HUANG Yong, CHEN Yue-Ying, HU Jun-Hua, GUO Xian-Guang, WANG Yue-Zhao. Geographic patterns and ecological factors correlates of snake species richness in China. Zoological Research, 2012, 33(4): 343-354.  doi: 10.3724/SP.J.1141.2012.04343
    [6] LI Song, YU Fa-Hong, LV Xue-Fei. Cranial morphometric study of four giant flying squirrels (Petaurista) (Rodentia: Sciuridae) from China. Zoological Research, 2012, (2): 119-126.  doi: 10.3724/SP.J.1141.2012.02119
    [7] XU Wei, Li Wei-Ye, WANG Yi-Quan. Identification and expressional analysis of green fluorescent protein genes in amphioxus. Zoological Research, 2012, 33(3): 304-313.  doi: 10.3724/SP.J.1141.2012.03304
    [8] LI Xin-Yi, LIN Yu-Shuang, ZHANG Hong-Wei. Phylogenetic analysis and expression patterns of tropomyosin in amphioxus. Zoological Research, 2012, 33(4): 389-394.  doi: 10.3724/SP.J.1141.2012.04389
    [9] ZHANG Qing-yi, CHENG Qi-qun, GUAN Wei-bing. Mitochondrial COI Gene Sequence Variation and Taxonomic Status of Three Macrobrachium Species. Zoological Research, 2009, 30(6): 613-619.  doi: 10.3724/SP.J.1141.2009.06613
    [10] ZHANG Qiu-jin, LI Guang, SUN Yi, WANG Yi-quan. Chromosome Preparation and Preliminary Observation of Two Amphioxus Species in Xiamen. Zoological Research, 2009, 30(2): 131-136.  doi: 10.3724/SP.J.1141.2009.02131
    [11] ZHANG Yu-jun, MAO Bing-yu. Developmental Expression of an Amphioxus (Branchiostoma belcheri) Gene Encoding a GATA Transcription Factor. Zoological Research, 2009, 30(2): 137-143.  doi: 10.3724/SP.J.1141.2009.02137
    [12] WEI Yun-hu, ZHANG Yu-jun, CHEN Yuan, MAO Bing-yu. Expansion of the Actin Gene Family in Amphioxus. Zoological Research, 2009, 30(5): 473-479.  doi: 10.3724/SP.J.1141.2009.05473
    [13] WANG Yi-quan , *, ZHANG Qiu-jin, LU Xiao-mei, ZHONG Jing, SUN Yi. Laboratory Culturing and Acquirement of the Second Filial Generation of Amphioxus. Zoological Research, 2006, 27(6): 631-634.
    [14] WANG Yi-quan, FANG Shao-hua. Taxonomic and Molecular Phylogenetic Studies of Amphioxus: A Review and Prospective Evaluation. Zoological Research, 2005, 26(6): 666-672.
    [15] BAO Lei, NIU Cui-juan, MA Rui LI Qing-fen. Sequences and Phylogeny of Partial Mitochondrial COⅠ Gene from Four Brachionus Species. Zoological Research, 2003, 24(3): 200-204.
    [16] ZHAO Ping, YUAN Xiao, TANG Si-xian, WANG Tian-hou. Species and Habitat Preference of Waterbirds at the Eastern End of Chongming Island (Shanghai) in Winter. Zoological Research, 2003, 24(5): 387-391.
    [17] LU Xue-Mei, WANG Ying-Xiang, ZHANG Ya-Ping. Divergence and Phylogeny of Mitochondrial Cytochrome b Gene from Species in Nycticebus. Zoological Research, 2001, 22(2): 93-98.
    [18] WENG You-Zhu, HUANG Wei-Quan, FANG Yong-Qiang, YAO Bing, SUN Lan. Study on GnRH Receptor (GnRHR) mRNA In situ Hybridization in The Nervous System,Hatschek's Pit and Gonad of Amphioxus,Branchiostoma belcheri. Zoological Research, 2000, 21(6): 437-441.
    [19] YE Chang-yuan, FEI Liang, CHEN Su-wen. Polymorphism and Geographic Distribution of Skin Texture of Six Species Amphibians in Hengduan Mountains. Zoological Research, 1994, 15(1): 33-45.
    [20] SHANG Jin-bao, LIU Wan-yi, QIN Shi-liang, CAI Jue. Studies on the Structure and Function of Amphioxus Ribosomes Ⅰ.Isolation of Amphioxus tRNAs and 5S RNAs. Zoological Research, 1982, 3(3): 317-321.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1780) PDF downloads(1598) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return