Volume 31 Issue 4
Jul.  2010
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LI Jun, GUO Xian-Guang, WANG Yue-Zhao. Population Genetic Differentiation of Phrynocephalus axillaris in East of Xinjiang Uygur Autonomous Region Based on Sequence Variation of Mitochondrial ND4-tRNALeu Gene. Zoological Research, 2010, 31(4): 361-369. doi: 10.3724/SP.J.1141.2010.04361
Citation: LI Jun, GUO Xian-Guang, WANG Yue-Zhao. Population Genetic Differentiation of Phrynocephalus axillaris in East of Xinjiang Uygur Autonomous Region Based on Sequence Variation of Mitochondrial ND4-tRNALeu Gene. Zoological Research, 2010, 31(4): 361-369. doi: 10.3724/SP.J.1141.2010.04361

Population Genetic Differentiation of Phrynocephalus axillaris in East of Xinjiang Uygur Autonomous Region Based on Sequence Variation of Mitochondrial ND4-tRNALeu Gene

doi: 10.3724/SP.J.1141.2010.04361
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  • Corresponding author: WANG Yue-Zhao,Tel:028-85217691
  • Received Date: 2010-01-25
  • Rev Recd Date: 2010-04-14
  • Publish Date: 2010-08-22
  • A 838 bp fragment of mtDNA ND4-tRNALeu gene was sequenced for 66 individuals from five populations (DB: Dabancheng, TU: Turpan, SS: Shanshan, HL: Liushuquan, HD: East district of Hami) of Phrynocephalus axillaris distributed in east of Xinjiang Uygur Autonomous Region. Seventeen haplotypes were identified from 29 nucleotide polymorphic sites in the aligned 838 bp sequence. Excluding DB, there were relatively high haplotype diversity (0.600±0.113) ≤ h ≤0(.922±0.012) and low nucleotide diversity (0.00082±0.00020) ≤ π≤(0.00917±0.00037) in the whole and the rest four populations. The result of phylogenetic analysis showed that the haplotypes from five populations formed cladeⅠ(DB+TU+SS) and cladeⅡ (HL+HD). Network analysis further revealed the phylogeographical structure of these five populations. Analysis of molecular variance (AMOVA) indicated that the significant genetic divergence (81.67%, P<0.01) existed among populations, which was not obviously correlated with the geographic distances between populations inferred from isolation-with-distance analysis (r=0.5324, P=0.1196). The demography analysis implied that TU and HL might have experienced the recent population expansion; SS and HD might be relatively stable. With extremely low genetic diversity, DB might be influenced by the special environment and be experiencing the bottleneck effect. It followed that the significant genetic differentiation among the five populations of P. axillaris might be the consequence of habitat changes resulting from the geological events and climate oscillation since Pleistocene and genetic drift.
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  • [1]
    Allen MB, Windley BF, Zhang C, Guo JH. 1993. Evolution of the Turpan basin, Chinese central Asian [J]. Tectonics, 12(4): 889-896.Arévalo E, Davis SK, Sites JW. 1994. Miotchondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in central Mexico [J]. Syst Biol, 43: 387-418.Avise JC, Arnold J, Ball RM, Bermingham E, Lamb T, Niegel JE, Reeb CA, Saunders NC. 1987. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics [J]. Annu Rev Ecol Syst, 18: 489-522.Bohonak AJ. 2002. IBD (Isolation By Distance): a program for analyses of isolation by distance [J]. J Hered, 93: 153-154.Cao SL, He DF. 1997. Evolution of the Caiwopu basin, Xinjiang [J]. Geotecton Metall, 21(3): 262-269. [曹守连, 何登发. 1997. 柴窝堡盆地的构造演化. 大地构造与成矿学, 21(3): 262-269.]Clement M, Posada D, Crandall KA. 2000. TCS: a computer program to estimate gene Genealogies [J]. Mol Ecol, 9(10): 1657-1660.Etterson JR, Shaw PE. 2001. Constraint to adaptive evolution in response to global warming [J]. Science, 294: 151-154.Excoffier L, Laval G, Schneider S. 2005. Arlequin 3.0: An integrated software package for population genetics data analysis [J]. Evol Bioinform Online, 1: 47-50.Frankham R. 1995. Conservation genetics [J]. Annu Rev Genet, 29: 305-327.Fu YX. 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection [J]. Genetics, 147: 915-925.Guindon S, Gascuel O. 2003. A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood [J]. Syst Biol, 52: 696-704.Guo XG, Wang YZ. 2007. Partitioned Bayesian analyses, dispersal-vicariance analysis,and the biogeography of Chinese toad-headed lizards (Agamidae: Phrynocephalus): A re-evaluation [J]. Mol Phylogenet Evol, 45: 643-662.Grant WS, Bowen BW. 1998. Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation [J]. J Hered, 89: 415-426.Hall TA. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT [J]. Nucleic Acids Symp Ser, 41: 95-98.Hartl DL, Clark AG. 1997. Principles of Population Genetics [M]. Massachusetts: Sinauer.Hewitt GM. 1996. Some genetic consequences of ice age, and their role in divergence and speciation [J]. Biol J Linn Soc, 58: 247-276.Hewitt GM. 1999. Postal-glacial re-colonization of European biota [J]. Biol J Linn Soc, 68: 87-112.Hewitt GM. 2000. The genetic legacy of the Quaternary ice ages [J]. Nature, 405: 907-913.Jian J. 2001. Basin analysis on Turpan-Hami basin [D]. Master thesis. Northwest University. [荐 军. 2001. 吐鲁番—哈密盆地盆地分析. 西北大学硕士学位论文.]Jin YT, Brown RP, Liu NF. 2008. Cladogenesis and phylogeography of the lizard Phrynocephalus vlangalii (Agamidae) on the Tibetan Plateau [J]. Mol Ecol, 17(8): 1971-1982.Jin YT, Liu NF. 2010. Phylogeography of Phrynocephalus erythrurus from the Qiangtang Plateau of the Tibetan Plateau [J]. Mol Phylogent Evol, 54(3): 933-940.Li M, Meng SJ, Wei FW, Wang J, Yong YG. 2003. Genetic diversity and population genetic structure of Takin (Budorcas taxicolor) [J]. Acta Theriol, 23(1): 10-16. [李 明, 蒙世杰, 魏辅文, 王 静, 雍严格. 2003. 羚牛的遗传多样性及其种群遗传结构分析. 兽类学报, 23(1): 10-16.]Librado P, Rozas J. 2009. DnaSP 5: A software for comprehensive analysis of DNA polymorphism data [J]. Bioinformatics, 25: 1451-1452.Liu L, Guo XG, Wang YZ. 2008. Genetic Variation and Diversity of Phrynocephalus vlangalii hongyuanensis in Zoige Wetland Inferred from ND4-tRNALeu Gene [J]. Zool Res, 29(2): 121-126. [刘 莉, 郭宪光, 王跃招. 2008. 若尔盖湿地青海沙蜥红原亚种线粒体ND4-tRNALeu基因的序列变异和遗传多样性. 动物学研究, 29(2): 121-126.]Posada D. 2008. jModelTest: Phylogenetic model averaging [J]. Mol Biol Evol, 25(7): 1253-1256.Petit RJ, Pineau E, Demesure B, Bacilieri R, Ducousso A, Kremer A. 1997. Chloroplast DNA footprints of postglacial recolonization by oaks [J]. Proc Natl Acad Sci USA, 94(18): 9996-10001.Rogers AR, Harpending H. 1992. Population growth makes waves in the distribution of pairwise genetic differences [J]. Mol Biol Evol, 9: 552-569.Ronquist F, Huelsenbeck JP. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models [J]. Bioinformatics, 19: 1572-1574.Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual [M].2nd ed. New York: Cold Spring Harbor Laboratory Press.Stone GN, Atkinson RJ, Brown G, Rokas A. 2002. The population genetic consequences of range expansion: A review of pattern and process, and the value of oak gallwasps as a model system [J]. Biodiv Sci, 10(1): 80-97.Swofford DL. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods) [M]. Version 4. Sunderland, Massachusetts: Sinauer Associates,Tamura K, Dudley J, Nei M. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version4.0 [J]. Mol Biol Evol, 24: 1596-1599.Wang JW. 1997. Paleogeorgaphical progresses in the formation of China arid land [J]. J Arid Land Resour Environ, 11(1): 1-8. [汪久文. 1997. 中国干旱区形成的古地理. 干旱区资源与环境, 11(1): 1-8.]Wang YZ, Zhan AB, Fu JZ. 2009. Testing historical phylogeographic inferences with contemporary gene flow data: Population genetic structure of the Qinghai toad-headed lizard [J]. J Zool, 278(2): 149-156.Wu HB, Guo ZT. 2000. Evolution and drought events in arid region of Northern China since the Last Glacial Maximum [J]. Quatern Sci, 20(6): 548-558. [吴海斌, 郭正堂. 2000. 末次盛冰期以来中国北方干旱区演化及短尺度干旱事件. 第四纪研究, 20(6): 548-558.]Wu GJ, Pan BT, Guan QY, Gao HS. 2002. The maximum glaciation and desert expansion in China during MIS16 [J]. J Glac Geocr, 24(5): 544-549. [邬光剑, 潘保田, 管清玉, 高红山. 2002. 中更新世全球最大冰期与中国沙漠扩张. 冰川冻土, 24(5): 544-549.]Xu GJ, Cai KZ, Shao GZ, Wang JP, Lin HD. 2005. Phylogeography and population genetic structure of Sinibrama macrops based on mtDNA [J]. BioFormosa, 40(2): 58-67. [许桂菁, 蔡昆展, 邵广昭, 王建平, 林弘都. 2005. 大眼华鳊亲缘地理与族群遗传结构之研究. 生物学报: 中国台湾, 40(2): 58-67.]Zhan SG. 2002. Formation and evolution of Turpan-Hami basin [J]. Fossils, 4: 27-29. [詹士高. 2002. 吐鲁番-哈密盆地的由来与演变. 化石, 4: 27-29.]Zhao WZ, Yuan F, Zeng XM. 1992. The structural characteristics of Turpan-Hami basin [J]. Acta Petrol Sin, 13(3): 9-18. [赵文智, 袁 非, 曾小明. 1992. 吐鲁番-哈密盆地的构造特征. 石油学报, 13(3): 9-18.]Zhao EM, Zhao KT, Zhou KY. 1999. Fauna Sinica. Reptilia: Vol. 2. Squamata. Lacertilia [M]. Beijing: Science Press, 151. [赵尔宓, 赵肯堂, 周开亚. 1999. 中国动物志 爬行纲: 第二卷 有鳞目 蜥蜴亚目[M]. 北京: 科学出版社, 151.]Zhu L. 2008. Preliminary study on molecular phylogeography of Phrynocephalus forsythii in Tarim Basin [D]. Master thesis, China West Normal University. [朱 磊. 2008. 塔里木盆地南疆沙蜥分子系统地理学研究初探. 西华师范大学硕士学位论文.]
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