Volume 30 Issue 4
Jul.  2009
Turn off MathJax
Article Contents

WU Xia, NIU Dong, LI Yan, YUE Yong-li, BOU Shorgan, YU Hai-quan. Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells. Zoological Research, 2009, 30(4): 361-368. doi: 10.3724/SP.J.1141.2009.04361
Citation: WU Xia, NIU Dong, LI Yan, YUE Yong-li, BOU Shorgan, YU Hai-quan. Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells. Zoological Research, 2009, 30(4): 361-368. doi: 10.3724/SP.J.1141.2009.04361

Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells

doi: 10.3724/SP.J.1141.2009.04361
More Information
  • Corresponding author: YU Hai-quan
  • Received Date: 2009-03-12
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-08-22
  • Trichostatin A, a histone deacetylase inhibitor, could increase histone acetylation, and active gene expression in somatic cells. However, effect of TSA on histone methylation is unclear. In present study, the bovine fibroblast cells were treated with TSA, and histone acetylation and methylation were examined by using indirect immunofluorescence and scanning confocal microscopy. We found that the morphology of cells was changed from being slightly concave to more flat and elongated even at the 10 ng/mL group. Intensive H4K8ac signals in fibroblast cells were observed after TSA treatment. A similar increasing with H4K8ac in H3K4me3 was found, but no change was observed at H3K9me2 when somatic cells were treated with TSA. These result indicated that TSA was not only increasing histone acetylation, but also increasing histone methylation that was correlated with gene expression. However, TSA could not change the level of histone methylation that regulated gene silence.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1564) PDF downloads(2166) Cited by()

Related
Proportional views

Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells

doi: 10.3724/SP.J.1141.2009.04361
    Corresponding author: YU Hai-quan

Abstract: Trichostatin A, a histone deacetylase inhibitor, could increase histone acetylation, and active gene expression in somatic cells. However, effect of TSA on histone methylation is unclear. In present study, the bovine fibroblast cells were treated with TSA, and histone acetylation and methylation were examined by using indirect immunofluorescence and scanning confocal microscopy. We found that the morphology of cells was changed from being slightly concave to more flat and elongated even at the 10 ng/mL group. Intensive H4K8ac signals in fibroblast cells were observed after TSA treatment. A similar increasing with H4K8ac in H3K4me3 was found, but no change was observed at H3K9me2 when somatic cells were treated with TSA. These result indicated that TSA was not only increasing histone acetylation, but also increasing histone methylation that was correlated with gene expression. However, TSA could not change the level of histone methylation that regulated gene silence.

WU Xia, NIU Dong, LI Yan, YUE Yong-li, BOU Shorgan, YU Hai-quan. Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells. Zoological Research, 2009, 30(4): 361-368. doi: 10.3724/SP.J.1141.2009.04361
Citation: WU Xia, NIU Dong, LI Yan, YUE Yong-li, BOU Shorgan, YU Hai-quan. Effect of Trichostatin A (TSA) on Histone Acetylation and Methylation in Bovine Fibroblast Cells. Zoological Research, 2009, 30(4): 361-368. doi: 10.3724/SP.J.1141.2009.04361

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return