Volume 30 Issue 2
Mar.  2009
Turn off MathJax
Article Contents
Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 2009, 30(2): 171-177. doi: 10.3724/SP.J.1141.2009.02171
Citation: Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 2009, 30(2): 171-177. doi: 10.3724/SP.J.1141.2009.02171

Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli

doi: 10.3724/SP.J.1141.2009.02171
More Information
  • Corresponding author: Shen Ji-hong, Lee Wen-hui
  • Received Date: 2008-11-26
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-04-22
  • OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobial drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coli happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died.
  • loading
  • Relative Articles

    [1] Fen Zhang, Zhi-Lai Guo, Yan Chen, Li Li, Hai-Ning Yu, Yi-Peng Wang. Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis. Zoological Research, 2019, 40(3): 198-204.  doi: 10.24272/j.issn.2095-8137.2018.070
    [2] James Mwangi, Xue Hao, Ren Lai, Zhi-Ye Zhang. Antimicrobial peptides: new hope in the war against multidrug resistance. Zoological Research, 2019, 40(6): 488-505.  doi: 10.24272/j.issn.2095-8137.2019.062
    [3] Feng Zhao, Xin-Qiang Lan, Yan Du, Pei-Yi Chen, Jiao Zhao, Fang Zhao, Wen-Hui Lee, Yun Zhang. King cobra peptide OH-CATH30 as a potential candidate drug through clinic drug-resistant isolates. Zoological Research, 2018, 39(2): 87-96.  doi: 10.24272/j.issn.2095-8137.2018.025
    [4] Bai-Yu ZHANG, Si-Man LI, Zheng-Hua GAO, Ji-Hong SHEN. Protective effects of snake venom antimicrobial peptide OH-CATH on E. coli induced rabbit urinary tract infection models. Zoological Research, 2013, 34(1): 25-32.  doi: 10.3724/SP.J.1141.2013.01027
    [5] GUANG Hui-Juan, LI Zheng, WANG Yi-Peng, LAI Ren, YU Hai-Ning. Progress in cathelicidins antimicrobial peptides research. Zoological Research, 2012, 33(5): 523-526.  doi: 10.3724/SP.J.1141.2012.05523
    [6] yu guo-Yu, XIANG Yang, ZHANG Hong-Yun, JIANG Ping, Lee Wen-Hui, ZHANG Yun, ZHANG. Expression of Bm-TFF2 mutants in Escherichia coli andtheir cell migration-promoting activity. Zoological Research, 2011, 32(4): 379-385.  doi: 10.3724/SP.J.1141.2011.04379
    [7] ZHANG Jie, YANG Mao-fa. Scanning Electron Microscopy of Antennae of Aphidoletes aphidimyza (Diptera: Cecidomyiidae). Zoological Research, 2008, 29(1): 108-112.
    [8] AN Xian-hui, LV Yi, LI Wei-guo, LIANG Jian-guo, XU Chun-hua, ZHANG Chong-xing et al.. Antimicrobial Peptides Purified from Penus chinensis. Zoological Research, 2005, 26(4): 410-415.
    [9] LAI Ren, LIANG Jian-guo, ZHANG Yun. Antimicrobial Peptides in Amphibian Skins and Their Application. Zoological Research, 2004, 25(5): 465-468.
    [10] LUO Hong, LIU Ci-quan, CAO Huai. Escherichia coli DNA Pribow and Transcription Initiation Sites A Statistical Study. Zoological Research, 1998, 19(4): 308-313.
    [11] ZHANG Zi-gui, DU Hong-yan, ZHANG Wei-nin, WU Fu-mei. Learning-Memory Dysfunction Caused by High Ca[2+] level and Changes of the Synaptic Interface-Structural Parameters. Zoological Research, 1998, 19(2): 120-124.
    [12] HAN Feng-ying. A Study on The Antennal Sensilla in Dragonflies Using Scanning Electron Microscopy. Zoological Research, 1997, 18(2): 156-162.
    [13] SAI Dao-jian, LI Liu-wen, LIU Lin-ying, SUN Jing-tian, WANG Shu-yun. Observation on The Eggshell of White-Fronted Shearwater With Scanning Electron Microscopy. Zoological Research, 1996, 17(1): 23-26.
    [14] FAN Pei-fang, YE Min-zhong, WIN Ting-huan. Scanning Electron Microscopy Observation on Demodex caprae. Zoological Research, 1996, 17(4): 385-391.
    [15] ZHENG Wen-biao, PAN Jiong-hua, AN Dong, LIU Wen-sheng. Observations on the Process of fertilization of Clarias lazera By Scanning Electron Microscopy. Zoological Research, 1991, 12(2): 111-115.
    [16] LI Chao-da, YANG Da-rong, SHEN Fa-rong, YANG Yao-xong. Scanning Electron Microscopy of Antennal Sensilla of the Yulong Ghost Moths,Hepialus yulongensis Liang. Zoological Research, 1990, 11(1): 83-86.
    [17] HOU Yi-di. Investigation of Infected State of Balantidium coli in Rhesus Monkey Groupos. Zoological Research, 1989, 10(zk): 151-155.
    [18] LI Jing-yan, CHEN Yun-he, ZHOU Xing-liang, ZHENG Zi-xiu. Immunoprotective Activity of Ribosomal Preparation From Poisonous Escherichia coli,the Pathogen of Piglet Dysentery. Zoological Research, 1984, 5(2): 169-174.
    [19] GAO Shun-de, ZHANG Hong-wei, HUANG Zhe. Scanning Electron Microscopy Study of the Surface Epithelium of Bursa of Fabricius in Chicken. Zoological Research, 1982, 3(3): 263-267.
    [20] XIA Bang-ying. Observation o the Chorion Structure of Ostrinia Nubilalis and Heliothis Armigera by Scanning and Transmission Electron Microscopy. Zoological Research, 1981, 2(4): 355-361.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1931) PDF downloads(2174) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint