Volume 30 Issue 2
Mar.  2009
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Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 2009, 30(2): 171-177. doi: 10.3724/SP.J.1141.2009.02171
Citation: Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We. Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli. Zoological Research, 2009, 30(2): 171-177. doi: 10.3724/SP.J.1141.2009.02171

Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli

doi: 10.3724/SP.J.1141.2009.02171
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  • Corresponding author: Shen Ji-hong, Lee Wen-hui
  • Received Date: 2008-11-26
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-04-22
  • OH-CATH is a novel cathelicidin identified from king cobra. It showed strong antibacterial activity against various bacteria in the presence of 1% NaCl and no haemolytic activity toward human red blood cells even at a high concentration. OH-CATH might serve as model molecules for the development of antimicrobial drugs. Understanding the action mechanism of OH-CATH and the reason for its selectivity against microbes is very important for this purpose. The bactericidal effect of the king cobra antimicrobial peptide OH-CATH on Gram-negative Escherichia coli (ATCC 25922) is observed by scanning electron microscopy (SEM) and transmitted electron microscopy (TEM). The SEM and TEM results suggested that the bactericidal mechanism of OH-CATH against Escherichia coli happened in three steps. Firstly, OH-CATH attached to the negatively charged bacterial wall by positively charged amino acid residues. In the second step, the accumulated OH-CATH aggregated and damaged the bacteria membrane in a pore-forming manner. In the last step, with the damage of cell permeability, the contents of the cells were released and eventually cells died.
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