Volume 30 Issue 2
Mar.  2009
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FENG Hao, LI Zong-jie, LV Yi, WANG Mei-juan, LI Jian-xu. Isolation and Molecular Cloning of Insulinotropic Peptide from the Skin Secretions of Amolops loloensis and the Study of Its Bioactivities. Zoological Research, 2009, 30(2): 165-170. doi: 10.3724/SP.J.1141.2009.02165
Citation: FENG Hao, LI Zong-jie, LV Yi, WANG Mei-juan, LI Jian-xu. Isolation and Molecular Cloning of Insulinotropic Peptide from the Skin Secretions of Amolops loloensis and the Study of Its Bioactivities. Zoological Research, 2009, 30(2): 165-170. doi: 10.3724/SP.J.1141.2009.02165

Isolation and Molecular Cloning of Insulinotropic Peptide from the Skin Secretions of Amolops loloensis and the Study of Its Bioactivities

doi: 10.3724/SP.J.1141.2009.02165
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  • Corresponding author: LI Jian-xu
  • Received Date: 2008-10-16
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-04-22
  • To investigate the bioactive component from the skin secretion of Amolops loloensis , isolated peaks with insulin-releasing activity were purified and structurally determined. Skin secretions were isolated by Gel filtration and Reversed phase high-performance liquid chromatography, and tested with insulin-releasing assay. The amino acid sequence of bioactive peak was determined by Edman degradation and identified by gene clone method. A 16-amino peptide with obvious insulin-releasing activity was obtained and sequenced as FMPIVGKSMSGLSGKL-NH2 , which was designated amolopin-1. The precursor peptide composed of 64 amino acid residues deduced from cloned skin cDNA (open reading frame 192 bp) exhibited a highly-conserved signal peptide (22aa), an acidic amino acid residue-rich domain and an amolopin-1 encoding domain. Structural alignment with database records revealed that a novel insulinotropic peptide was obtained from skin secretion of Amolops loloensis therefore further study of its acting mechanism and pharmacokinetics may lead to the discovery of a new treatment for diabetes.
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