Volume 42 Issue 2
Mar.  2021
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Yun Zhang, Qi-Quan Wang, Zhong Zhao, Cheng-Jie Deng. Animal secretory endolysosome channel discovery. Zoological Research, 2021, 42(2): 141-152. doi: 10.24272/j.issn.2095-8137.2020.358
Citation: Yun Zhang, Qi-Quan Wang, Zhong Zhao, Cheng-Jie Deng. Animal secretory endolysosome channel discovery. Zoological Research, 2021, 42(2): 141-152. doi: 10.24272/j.issn.2095-8137.2020.358

Animal secretory endolysosome channel discovery

doi: 10.24272/j.issn.2095-8137.2020.358
#Authors contributed equally to this work
Funds:  This work was supported by the National Natural Science Foundation of China (31572268, U1602225, 31872226) and Yunling Scholar Program to Y.Z.
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  • Corresponding author: E-mail: zhangy@mail.kiz.ac.cn
  • Received Date: 2020-12-09
  • Accepted Date: 2021-01-29
  • Available Online: 2021-02-01
  • Publish Date: 2021-03-18
  • Secretory pore-forming proteins (PFPs) have been identified in organisms from all kingdoms of life. Our studies with the toad species Bombina maxima found an interaction network among aerolysin family PFPs (af-PFPs) and trefoil factors (TFFs). As a toad af-PFP, BmALP1 can be reversibly regulated between active and inactive forms, with its paralog BmALP3 acting as a negative regulator. BmALP1 interacts with BmTFF3 to form a cellular active complex called βγ-CAT. This PFP complex is characterized by acting on endocytic pathways and forming pores on endolysosomes, including stimulating cell macropinocytosis. In addition, cell exocytosis can be induced and/or modulated in the presence of βγ-CAT. Depending on cell contexts and surroundings, these effects can facilitate the toad in material uptake and vesicular transport, while maintaining mucosal barrier function as well as immune defense. Based on experimental evidence, we hereby propose a secretory endolysosome channel (SELC) pathway conducted by a secreted PFP in cell endocytic and exocytic systems, with βγ-CAT being the first example of a SELC protein. With essential roles in cell interactions and environmental adaptations, the proposed SELC protein pathway should be conserved in other living organisms.
  • #Authors contributed equally to this work
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