Volume 42 Issue 2
Mar.  2021
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Article Contents
Ren-Rong Tian, Cui-Xian Yang, Mi Zhang, Xiao-Li Feng, Rong-Hua Luo, Zi-Lei Duan, Jian-Jian Li, Jia-Fa Liu, Dan-Dan Yu, Ling Xu, Hong-Yi Zheng, Ming-Hua Li, Hong-Li Fan, Jia-Li Wang, Xing-Qi Dong, Yong-Tang Zheng. Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study. Zoological Research, 2021, 42(2): 161-169. doi: 10.24272/j.issn.2095-8137.2020.329
Citation: Ren-Rong Tian, Cui-Xian Yang, Mi Zhang, Xiao-Li Feng, Rong-Hua Luo, Zi-Lei Duan, Jian-Jian Li, Jia-Fa Liu, Dan-Dan Yu, Ling Xu, Hong-Yi Zheng, Ming-Hua Li, Hong-Li Fan, Jia-Li Wang, Xing-Qi Dong, Yong-Tang Zheng. Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study. Zoological Research, 2021, 42(2): 161-169. doi: 10.24272/j.issn.2095-8137.2020.329

Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study

doi: 10.24272/j.issn.2095-8137.2020.329
#Authors contributed equally to this work
Funds:  This work was supported in part by the National Key R&D Program of China (2020YFC0842000 and 2020YFC0847000 to Y.T.Z.), Yunnan Provincial Major Science Technology Project (202003AC100008 to X.Q.D.), and Key Research Program of the Chinese Academy of Sciences (KJZD-SW-L11 to Y.T.Z.)
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  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease 2019 (COVID-19) continue to impact countries worldwide. At present, inadequate diagnosis and unreliable evaluation systems hinder the implementation and development of effective prevention and treatment strategies. Here, we conducted a horizontal and longitudinal study comparing the detection rates of SARS-CoV-2 nucleic acid in different types of samples collected from COVID-19 patients and SARS-CoV-2-infected monkeys. We also detected anti-SARS-CoV-2 antibodies in the above clinical and animal model samples to identify a reliable approach for the accurate diagnosis of SARS-CoV-2 infection. Results showed that, regardless of clinical symptoms, the highest detection levels of viral nucleic acid were found in sputum and tracheal brush samples, resulting in a high and stable diagnosis rate. Anti-SARS-CoV-2 immunoglobulin M (IgM) and G (IgG) antibodies were not detected in 6.90% of COVID-19 patients. Furthermore, integration of nucleic acid detection results from the various sample types did not improve the diagnosis rate. Moreover, dynamic changes in SARS-CoV-2 viral load were more obvious in sputum and tracheal brushes than in nasal and throat swabs. Thus, SARS-CoV-2 nucleic acid detection in sputum and tracheal brushes was the least affected by infection route, disease progression, and individual differences. Therefore, SARS-CoV-2 nucleic acid detection using lower respiratory tract samples alone is reliable for COVID-19 diagnosis and study.
  • #Authors contributed equally to this work
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