Volume 36 Issue 1
Jan.  2015
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Fang DING, Li CHEN, Yong LIU, Feng-Rui WU, Biao DING, Wen-Yong LI, Rong WANG. Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos. Zoological Research, 2015, 36(1): 54-58. doi: 10.13918/j.issn.2095-8137.2015.1.54
Citation: Fang DING, Li CHEN, Yong LIU, Feng-Rui WU, Biao DING, Wen-Yong LI, Rong WANG. Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos. Zoological Research, 2015, 36(1): 54-58. doi: 10.13918/j.issn.2095-8137.2015.1.54

Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos

doi: 10.13918/j.issn.2095-8137.2015.1.54
Funds:  This study was supported by the National Natural Science Foundation of China (31372273, 31201789); Academic Renovation Research Project of Anhui University (yqh100125); Natural Science Foundation of the Education Bureau of Anhui Province (KJ2013A202); Major project of discipline construction in Anhui province ([2014]No.28); and Natural Science Foundation of Anhui Province (1408085MC44, 1408085QC65)
More Information
  • Corresponding author: Rong WANG
  • Received Date: 2014-07-14
  • Rev Recd Date: 2014-10-25
  • Publish Date: 2015-01-08
  • It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels (except at the 2-cell stage). This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early pre-implantation mouse embryos.
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  • [1]
    Dole VP, Gentry RT. 1984. Toward an analogue of alcoholism in mice: scale factors in the model. Proceedings of the National Academy of Sciences of the United States of America, 81(11): 3543-3546.
    [2]
    Dole VP, Ho A, Gentry RT. 1985. Toward an analogue of alcoholism in mice: criteria for recognition of pharmacologically motivated drinking. Proceedings of the National Academy of Sciences of the United States of America, 82(10): 3469-3471.
    [3]
    Fisher SE, Duffy L, Atkinson M. 1986. Selective fetal malnutrition: effect of acute and chronic ethanol exposure upon rat placental Na, K-ATPase activity. Alcoholism: Clinical and Experimental Research, 10(2): 150-153.
    [4]
    Griffin WC III, Lopez MF, Becker HC. 2009. Intensity and duration of chronic ethanol exposure is critical for subsequent escalation of voluntary ethanol drinking in mice. Alcoholism, Clinical and Experimental Research, 33(11): 1893-1900.
    [5]
    Haycock PC. 2009. Fetal alcohol spectrum disorders: the epigenetic perspective. Biology of Reproduction, 81(4): 607-617.
    [6]
    Huang JC, Wang CH, Liu Y, Wu FR, Li WY, Wang R. 2013a. Chronic alcohol ingestion influences sperm semen quality and global DNA methylation in pre-implantation embryos. Chinese Journal of Cell Biology, 35(8): 1103-1109. (in Chinese)
    [7]
    Huang JC, Wang CH, Liu Y, Wu FR, Ding B, Li WY, Wang R. 2013b. Female rats ingested alcohol before and after pregnancy impact DNA methylation patterns in pre-implantation embryo. Chinese Journal of Cell Biology, 35(12): 1765-1771. (in Chinese)
    [8]
    Hwa LS, Chu A, Levinson SA, Kayyali TM, Debold JF, Miczek KA. 2011. Persistent escalation of alcohol drinking in C57BL/6J mice with intermittent access to 20% ethanol. Alcoholism, Clinical and Experimental Research, 35(11): 1938-1947.
    [9]
    Jiang QY, Hu YQ, Cheng XS, Deng JB. 2007. Neuroapoptosis in visual cortex of offspring mouse after prenatal ethanol exposure. Acta Anatomica Sinica, 38(4): 400-404. (in Chinese)
    [10]
    Kidder GM. 1993. Genes involved in cleavage, compaction, and blastocyst formation. In: Gwatkin RBL. Genes in Mammalian Reproduction. New York: Wiley-Liss, 45-47.
    [11]
    Kim JM, Ogura A, Nagata M, Aoki F. 2002. Analysis of the mechanism for chromatin remodeling in embryos reconstructed by somatic nuclear transfer. Biology of Reproduction, 67(3): 760-766.
    [12]
    Li YM. 2003. New insight on the pathogenesis of alcoholic liver diseases. Chinese Journal of Hepatalogy, 11(11): 690-691. (in Chinese)
    [13]
    Lin GW. 1981. The effect of ethanol consumption during gestation on maternal-fetal amino acid metabolism in the rat. Currents in Alcoholism, 8: 479-483.
    [14]
    Mattson SN, Crocker N, Nguyen TT. 2011. Fetal alcohol spectrum disorders: neuropsychological and behavioral features. Neuropsychology Review, 21(2): 81-101.
    [15]
    Ma J, Svoboda P, Schultz RM, Stein P. 2001. Regulation of zygotic gene activation in the preimplantation mouse embryo: global activation and repression of gene expression. Biology of Reproduction, 64(6): 1713-1721.
    [16]
    Morinobu A, Kanno Y, O'shea JJ. 2004. Discrete roles for histone acetylation in human T helper 1 cell-specific gene expression. The Journal of Biological Chemistry, 279(39): 40640-40646.
    [17]
    Pan B, Sun HC, Lv TW, Wu XY, Zhu J, Tian J. 2013. Hyperacetylation imbalance and epimutation of H3K9 induced by alcoholconsumed during gestation in mice. Journal of Clinical Cardiology, 29(5): 395-398. (in Chinese)
    [18]
    Qiu JJ, Zhang WW, Wu ZL, Wang YH, Qian M, Li YP. 2003. Delay of ZGA initiation occurred in 2-cell blocked mouse embryos. Cell Research, 13(3): 179-185.
    [19]
    Tang GE. 2006. Detection of Reactive Oxygen Species (ROS) in Different Developmental Stage Mouse Embryos in Vivo and in vitro. Master Thesis, Agriculture College of Yanbian University, Jilin. (in Chinese)
    [20]
    Vigushin DM, Coombes RC. 2002. Histone deacetylase inhibitors in cancer treatment. Anti-cancer Drugs, 13(1): 1-13.
    [21]
    Wang CH, Liu Y, Huang JC, Wu FR, Ding B, Zhang Y, Wang R, Li WY. 2013. The difference of the patterns of H3K27 acetylation between parthenogenetic embryos and in vivo mouse embryos and the effect on development. Sichuan Journal of Zoology, 32(5): 684-688. (in Chinese)
    [22]
    Yeh CH, Chang JK, Wang YH, Ho ML, Wang GJ. 2008. Ethanol may suppress Wnt/β-catenin signaling on human bone marrow stroma cells: a preliminary study. Clinical Orthopaedics and Related Research, 466(5): 1047-1053.
    [23]
    Zhao DM, Xu CM, Huang HF, Qian YL, Jing F. 2005. Expression patterns of GCN5 and HDAC1 in preimplantational mouse embryos and effects of in-vitro cultures on their expressions. Acta Biologiae Experimentalis Sinica, 38(6): 513-519. (in Chinese)
    [24]
    Zhao MQ, Feng LD. 2012. Biomarkers of alcohol binge-drinking Induced brain damage in mice. Journal of Medical Research, 41(8): 169-171. (in Chinese)
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