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Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews

Xiao-Yan HUANG Ming-Li LI Juan XU Yue-Dong GAO Wen-Guang WANG An-Guo YIN Xiao-Fei LI Xiao-Mei SUN Xue-Shan XIA Jie-Jie DAI

Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews. Zoological Research, 2013, 34(2): 121-126. doi: 10.3724/SP.J.1141.2013.02121
Citation: Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews. Zoological Research, 2013, 34(2): 121-126. doi: 10.3724/SP.J.1141.2013.02121

树鼩IL-2全长编码序列的克隆及分子特征分析

doi: 10.3724/SP.J.1141.2013.02121
基金项目: 国家科技支撑计划项目 (2009BAI83B02-21;2011BAI15B01-21;2012BAI39B01);云南省科技计划重点项目(2006PT07-2);云南省应用基础面上项目(2011FZ211)。
详细信息
  • 中图分类号: Q95-33

Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews

  • 摘要: 树鼩作为多种人类疾病模型已受到广泛关注, 而免疫因子对于树鼩模型评价至关重要, 但目前对其白细胞介素-2(IL-2)的研究鲜有报道。该实验以经ConA (concanavalin)诱导培养的树鼩淋巴细胞总RNA 为模板, RT-PCR 克隆出465 bp 的树鼩IL-2全长编码序列, 并采用Clustal W 软件分析其序列和分子特征。结果表明树鼩IL-2 cDNA 编码一个由154 个氨基酸组成的蛋白质, 其cDNA 及氨基酸序列与人的同源性分别为93%及80%, 且其整体结构与人IL-2 相似。MEGA5.0 软件构建的进化树表明, 树鼩与人及恒河猴的亲缘关系较近。Pymol 软件对树鼩和人IL-2 氨基酸序列进行的三维结构模建表明, 两者的IL-2分子三维空间结构基本相似, 表面大部分区域所带电荷相同, 但在某些区域差异较大, 且树鼩多出一个糖基化位点, 这些差异对抗体的结合可能存在影响。该研究为今后树鼩IL-2 单克隆抗体的制备及功能研究奠定了基础。
  • [1] Amako Y, Tsukiyama-Kohara K, Katsume A, Hirata Y, Sekiquchi S, Tobita Y, Hayashi Y, Hishima T, Funata N, Yonekawa M. 2010. Pathogenesis of hepatitis C virus infection in Tupaia belangeri. Journal of Virology, 84(1): 303-311.
    [2] Bozkaya H, Bozdayi AM, Aslan N, Türkay C, Sarioglu M, ?etinkaya H, Akdogan M, Cinar K, Erden E, Kose K, Senturk H, Akkiz H, Karayalcin S, Yurdaydin C, Uzunalimoglu O. 2000. Circulating IL-2 and IL-10 in chronic active hepatitis C with respect to the response to IFN treatment. Infection, 28(5): 309-313.
    [3] Cao J, Yang EB, Su JJ, Li Y, Chow P. 2003. The tree shrews: Adjuncts and alternatives to primates as models for biomedical research. Journal of Medical Primatology, 32(3): 123-130.
    [4] Church A C. 2003. Clinical advances in therapies targeting the interleukin-2 receptor. QJM: An International Journal of Medicine, 96(2): 91-102.
    [5] Fukuda R, Ishimura N, Ishara S, Chowdhury A, Morlyama N, Nogami C, Miyake T, Niigaki M, Tokuda A, Satoh S, Sakai S, Akagi S, Watanabe M, Fukumoto S. 1996. Intrahepatic expression of pro-inflammatory cytotine mRNA and interferon efficacy in chronic hepatitis C. Liver, 16: 390-399.
    [6] Gillis S, Union NA, Baker PE, Smith KA. 1979. The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes. The Journal of Experimental Medicine, 149(6): 1460-1476.
    [7] Lenardo M J. 1991. Interleukin-2 programs mouse alpha beta T lymphocytes for apoptosis. Nature, 353(6347): 858-861.
    [8] Lis H, Sharon N. 1993. Protein glycosylation. Structural and functional aspects. European Journal of Biochemistry, 218(1): 1-27.
    [9] Morgan DA, Ruscetti FW, Gallo R. 1976. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science, 193(4257): 1007-1008.
    [10] Napoli J, Bishop GA, McGuinness PH, Painter DM, McCaughan GW. 1996. Progressive liver injury in chronic hepatitis C infection correlates with increased intrahepatic expression of Th1-associated cytokines. Hepatology, 24(4): 759-765.
    [11] Novacek MJ. 1992. Mammalian phylogeny: shaking the tree. Nature, 356(6365): 121-125.
    [12] Nie W, Fu B, O'Brien PC, Wang J, Su W, Tanomtong A, Volobouev V, Ferguson-Smith MA, Yang F. 2008. Flying lemurs—the‘flying tree shrews’? Molecular cytogenetic evidence for a Scandentia-Dermoptera sister clade. BMC biology, 6:18.
    [13] Shanafelt AB, Lin Y, Shanafelt MC, Forte CP, Dubois-Stringfellow N, Carter C, Gibbons JA, Cheng SL, Delaria KA, Fleischer R, Greve JM, Gundel R, Harris K, Kelly R, Koh B, Li Y, Lantz L, Mak P, Neyer L, Plym MJ, Roczniak S, Serban D, Thrift J, Tsuchiyama L, Wetzel M, Wong M, Zolotorev A. 2000. A T-cell-selective interleukin-2 mutein exhibits potent antitumor activity and is well tolerated in vivo. Nature Biotechnol, 18(11): 1197-1202.
    [14] Suzuki S, Konnai S, Okagawa T, Githaka NW, Kariukit E, Gakuyat F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K. 2012. Molecular cloning and characterization of Th1 and Th2 cytokines of African buffalo(Syncerus Caffer). International Journal of Immunogenetics, 39(2): 170-182.
    [15] Taniguchi T, Matsui H, Fujita T, Takaoka C, Kashima N, Yoshimoto R, Hamuro J. 1983. Structure and expression of a cloned cDNA for human interleukin-2. Nature, 302(5906): 305-310.
    [16] Theze J, Alzari PM, Bertoglio J. 1996. Interleukin 2 and its receptors: recent advances and new immunological functions. Immunology Today, 17(10): 481-486.
    [17] Voss SD, Sondel PM, Robb RJ. 1992. Characterization of the interleukin-2 receptors(IL-2R) expressed on human nature killer cells activated in vivo by IL-2: association of the p64 IL-2R gamma chain with the IL-2R beta chain in functional intermediate-affinity IL-2R. The Journal of Experimental Medicine, 176(2): 531-541.
    [18] Wang HP, Zhou YX, Yao ZQ, Hong S, Li GY. 1997. The preliminary study of infection of major tupaia with hepatitis C virus. Journal of the Fourth Military Medical University, 18(4): 375-376. [王海平, 周永兴, 姚志强, 洪沙, 李光玉. 1997. 成年树鼩实验感染丙型肝炎病毒的初步研究. 第四军医大学学报, 18(4): 375-376]
    [19] Wang X, Rickert M, Garcia KC. 2005. Structure of the quaternary complex of interleukin-2 with its α, β and γc receptors. Science, 310(5751): 1159-1163.
    [20] Zelus D, Robinson-Rechavi M, Delacre M, Auriault C, Laudet V. 2000. Fast evolution of interleukin-2 in mammals and positive in ruminants. Journal of Molecular Evolution, 51(3): 234-244.
    [21] Zhao XP, Tian ZF, Chen YC, Yang C, Tian DY, Yang DL, Hao LJ. 2005. Infection of tupaia hepatocytes with hepatitis C virus in vitro. Chinese Journal of Hepatology, 13(11): 805-807. [赵西平, 田展飞, 陈义春, 杨春, 田德英, 杨东亮, 郝连杰. 2005. 丙型肝炎病毒体外可感染树鼩肝细胞. 中华肝脏病杂志, 13(11): 805-807.
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  • 收稿日期:  2012-12-03
  • 修回日期:  2013-01-04
  • 刊出日期:  2013-04-08

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