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Cryopreservation of sperm from Neolissochilus benasi

WANG Xiao-Ai YANG Jun-Xing CHEN Xiao-Yong PAN Xiao-Fu LI Zai-Yun

WANG Xiao-Ai, YANG Jun-Xing, CHEN Xiao-Yong, PAN Xiao-Fu, LI Zai-Yun. Cryopreservation of sperm from Neolissochilus benasi. Zoological Research, 2012, 33(3): 283-289. doi: 10.3724/SP.J.1141.2012.03283
Citation: WANG Xiao-Ai, YANG Jun-Xing, CHEN Xiao-Yong, PAN Xiao-Fu, LI Zai-Yun. Cryopreservation of sperm from Neolissochilus benasi. Zoological Research, 2012, 33(3): 283-289. doi: 10.3724/SP.J.1141.2012.03283

软鳍新光唇鱼精子的超低温冷冻保存

doi: 10.3724/SP.J.1141.2012.03283
基金项目: 云南大唐国际李仙江流域水电开发有限公司委托项目;国家电力云南阿墨江发电有限公司委托项目
详细信息
  • 中图分类号: Q959.468;Q954.43;Q492

Cryopreservation of sperm from Neolissochilus benasi

  • 摘要: 2011年,对软鳍新光唇鱼(Neolissochilus benasi)进行了精子超低温冷冻保存研究。以解冻后的精子活力为指标, 采用稀释液D-15, 设计不同的抗冻剂种类和浓度, 以及不同的实验条件(包括冷冻体积、4℃平衡时间和解冻温度等)探索软鳍新光唇鱼精子的超低温冷冻保存方法。筛选出了适合软鳍新光唇鱼精子超低温冷冻保存的两种抗冻保护剂及其浓度分别为10% MeOH 和15% EG, 确定了精液与稀释液的最适稀释比例为1:7、4℃平衡时间区间为10~60 min、冷冻体积为60μL, 以及复苏方法为37℃水浴快速解冻30s。当鲜精活力为(62.33±2.05)%,综合以上最佳实验条件进行保存, 解冻后精子的最高活力为(29.67±0.47)%, 但效果不理想, 不能达到广泛生产运用水平; 产生这一结果, 可能与异地保育物种的饲养管理有关。因此, 在亲鱼培育管理中要最大限度地降低捕获诱发的压力, 尽量提供适合的养殖条件。在珍稀鱼类异地保育时, 繁殖用雄鱼的培育与雌鱼同等重要, 是获得大量高质量仔鱼的关键。
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  • 收稿日期:  2011-11-04
  • 修回日期:  2012-02-14
  • 刊出日期:  2012-06-22

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