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Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose

SONG Hai-Yan LIU Zai-Qun ZHENG Lei

SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211
Citation: SONG Hai-Yan, LIU Zai-Qun, ZHENG Lei. Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose. Zoological Research, 2012, (2): 211-217. doi: 10.3724/SP.J.1141.2012.02211

IFN-γ、IL-1α、NGF-β和TNF-α在皖西白鹅小脑皮质发育中的表达

doi: 10.3724/SP.J.1141.2012.02211
基金项目: 安徽师范大学博士科研启动基金(160-750932),生科院联合开放课题经费; “安徽重要生物资源保护与利用研究”安徽省重点实验室科研基金
详细信息
  • 中图分类号: Q426; Q593+.2; Q915.865

Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white goose

  • 摘要: 用免疫组织化学streptactividin-biotincomplex(SABC)法,以干扰素-γ(IFN-γ)、白介素-1α(IL-1α)、神经生长因子-β(NGF-β)和肿瘤坏死因子-α(TNF-α)对胚龄13d、19d、24d、28d(E13、E19、E24、E28)和日龄7d、15d(P7、15)的皖西白鹅(WesternAnhuiwhitegoose)小脑皮质中的阳性细胞进行定位和半定量检测,探讨IFN-γ、IL-1α、NGF-β和TNF-α在小脑皮质发育中的作用。研究表明,外颗粒层细胞在E13、E19、E24、E28、P7有IFN-γ和TNF-α阳性表达;在E13、E19、E24、E28有IL-1α阳性表达;在E13、E19、E24有NGF-β阳性表达;且在所检测的6个时期中,4种细胞因子均在E19表达最强。Purkinje细胞层在E13、E19、E24、E28、P7、P15均有IFN-γ、IL-1α、TNF-α阳性表达;在E13、E19、E24、E28、P7有NGF-β阳性表达;内颗粒层细胞在E13、E19、E24、E28、P7、P15有IFN-γ阳性表达;在E13、E19、E24、E28、P7有IL-1α、TNF-α阳性表达;在E13、E19、E24、E28有NGF-β阳性表达。结果表明,E19可能为小脑皮质发育的“关键期”;IFN-γ、IL-1α和TNF-α可能由小脑皮质自身合成;NGF-β可能由投射到Purkinje细胞的区域转运而来,且可能在Purkinje细胞生长发育过程中起营养作用;IFN-γ可能在颗粒细胞迁移过程中起干扰作用。
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  • 收稿日期:  2011-12-01
  • 修回日期:  2012-01-09
  • 刊出日期:  2012-04-22

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