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Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining

GAO Xin ZHU Jia-Jun YANG Xian-Yu YUAN Jin-Qiang WANG Yi-Wen SONG Min-Guo

GAO Xin, ZHU Jia-Jun, YANG Xian-Yu, YUAN Jin-Qiang, WANG Yi-Wen, SONG Min-Guo. Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining. Zoological Research, 2011, 32(4): 461-464. doi: 10.3724/SP.J.1141.2011.04461
Citation: GAO Xin, ZHU Jia-Jun, YANG Xian-Yu, YUAN Jin-Qiang, WANG Yi-Wen, SONG Min-Guo. Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining. Zoological Research, 2011, 32(4): 461-464. doi: 10.3724/SP.J.1141.2011.04461

免疫荧光染色揭示草履虫接合生殖过程中静止原核的空间位置(英文)

doi: 10.3724/SP.J.1141.2011.04461
基金项目: 国家自然基金项目(30670397; 31071181); 教育部留学回国人员科研启动基金项目([2007]1108); 浙江省教育厅一般项目 (Y200804256); 浙江农林大学科研发展基金项目(2292000030)
详细信息
    作者简介:

    高欣,男,讲师;研究方向:动物学;E-mail:wildlife909@yahoo.cn

    通讯作者:

    杨仙玉

  • 中图分类号: 草履虫; 接合生殖; 核间连丝; 原核

Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining

More Information
    Author Bio:

    GAO Xin,E-mail:wildlife909@yahoo.cn

    Corresponding author: YANG Xian-Yu
  • 摘要: 在尾草履虫的接合生殖过程中, 共有三次配前核分裂。在配前第三次分裂结束后, 两个接合的细胞内均形成一个迁移原核和一个静止原核。迁移原核位于口旁锥内,而且紧贴于接合面, 静止原核则位于迁移原核的外侧, 两者呈左右排列, 距离接近。但是, 目前对导致两种原核近距离的原因尚不清楚。该文通过α-微管蛋白的单克隆抗体对受精核形成前的接合对进行了免疫荧光染色, 结果发现,配前第三次分裂不同于前两次分裂, 连接迁移原核和静止原核的核间连丝伸向细胞的后方, 呈“U”或“V”型, 结果导致两个原核左右排列, 而不是前后排列, 两者间的距离缩短。这个结果也阐明了造成两种原核近距离的原因。
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  • [1] Yiwen WANG, Jinqiang YUAN, Xin GAO, Xianyu YANG*.  Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium, Zoological Research. doi: 10.3724/SP.J.1141.2012.E05-06E98
    [2] GAO Xin, YANG Xian-Yu, ZHU Jia-Jun, YUAN Jin-Qiang, WANG Yi-Wen, SONG Min-Guo.  New details indicated by different stainings during conjugation of ciliated protozoa Paramecium, Zoological Research. doi: 10.3724/SP.J.1141.2011.06651
    [3] SUN Jun, ZHAI Wan-yin, DING Xiang-huan, GU Fu-kang.  Effects of Symbiont on the Morphology,Growth,Resistance to Oxidation,and Saccharide Content of Hosts in the Paramecium bursaria-Chlorella System, Zoological Research.
    [4] JIN Li-Pei, LIU Xiao-Yi, JIN Hua-Zhong.  Nuclear Control of Cortical Development during Conjugation in Pseudourostyla cristata, Zoological Research.
    [5] SUN Jun, GU Fu-Kang.  Diversity of Genomic DNA of Chlorellae-Bearing and Chlorellae-Free Paramecium bursaria, Zoological Research.
    [6] ZHANG Zuo-ren, ZHUANG Hao, JIN Li-pei.  Studies on Conjugation in Pseudourostyla cristata Ⅱ.Morphogenesis of Cortical Structures, Zoological Research.
    [7] JIN Li-pei, ZHUANG Hao, Tchang Tsorun.  Studies on Conjugation in Pseudourostyla cristata Ⅰ.Nucleogenesis, Zoological Research.
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出版历程
  • 收稿日期:  2011-01-24
  • 修回日期:  2011-06-28
  • 刊出日期:  2011-08-22

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