Preliminary investigation of viruses to the wild tree shrews (Tupaia belangeri chinese)
-
摘要: 病毒学检测和监测是树鼩实验动物化和质量控制的重要标准和依据,而野生中缅树鼩是否携带人兽共患病毒鲜见报道。本研究采用酶联免疫吸附(ELISA)方法, 对来源于云南昆明市城郊青龙峡地区的野生树鼩是否携带单纯疱疹病毒、轮状病毒、流感病毒、柯萨奇病毒、甲肝病毒、乙肝病毒、丙肝病毒、丁肝病毒、登革热病毒、出血热病毒和麻疹病毒等11种常见病毒进行筛查。结果表明, 在已筛查的60只野生中缅树鼩中,可检测到单纯疱疹病毒和柯萨奇病毒,其血清抗体阳性比例分别为36.7% (22/60) 和1.67% (1/60),而在粪便中仅检测到轮状病毒,其抗原阳性为6.7% (4/60), 未检测到其他病毒,初步显示了野生树鼩自然状态下携带病毒的状况。为此,建议将单纯疱疹病毒、柯萨奇病毒和轮状病毒列为普通级树鼩病毒质量控制的首检项目,进一步大样本筛查将显示是否将其他病毒列为必检项目。Abstract: Virological testing and monitoring is a fundamental part of quality control of experimental animals. However, there are few papers regarding the spectrum and status of natural infection in wild tree shrews with human and animal pathogenic viruses. Using enzyme-linked immunosorbent adsorption assay (ELISA), we tested sixty wild tree shrews captured from Qinglong, an outskirt region of Kunming, Yunnan Province, China for eleven viruses, including herpes simplex virus, coxsackie virus, influenza virus, HAV, HBV, HCV, HDV, dengue virus, hemorrhagic fever virus and measles virus. Our results showed that, in the serum samples, 22/60 (36.7%) and 1/60 (1.67%) were antibody positive for herpes simplex virus and coxsackie virus, respectively, and 4/60 (6.7%) were antigen positive for rotavirus in the feces. The remaining species of viruses were negative in these tree shrews. Based on these results, we propose that herpes simplex virus, coxsackie virus and cotavirus should be listed as top priority for routine virological monitoring of tree shrews.
-
Key words:
- Tree shrew (Tupaia belangeri chinese) /
- Viruses /
- Virological monitoring
-
[1] Chen J, Dai JJ, Sun XM. 2008. Advances in the use of tree shrews as animal models for hepatitis research[J]. Chin J Compar Med, 18(2): 59-62. [2] [陈瑾, 代解杰, 孙晓梅. 2008. 树鼩肝炎动物模型的研究进展.中国比较医学杂志. 18(2): 59-62.]Daral G, Zöller L, Matz B, Schwaier A, Flügel RM, Munk K. 1980. Experimental infection and the state of viral latency of adult Tupaia with herpes simplex virus tape 1 and 2 and infection of juvenile Tupaia with temperature-sensitive mutants of HSV type 2[J]. Arch Virol, 65: 311-318.中华人民共和国国家质量监督检验检疫总局. 2002. GB/T14926.1-14926.41-2001[S]. 实验动物微生物学检验方法. 北京:中国国家标准化管理委员会.中华人民共和国国家质量监督检验检疫总局. 2002. GB14922.2-2001[S]. 实验动物微生物学等级及监测.北京:中国国家标准化管理委员会.He ZM. 2010. Potentials and prospects: Laboratory animal derived from resource animals in China[J]. Chin J Compar Med, 20(3): 1-7. [3] [贺争鸣. 2010. 我国资源动物的实验动物化潜力与展望. 中国比较医学杂志, 20(3): 1-7.]Hu XL, Li JQ. 2002. Application of Tupaia on studying of virus disease[J]. Virol Sin, 17(3): 276-278. [4] [胡溪柳, 李际强. 2002. 树鼩应用于病毒学方面的研究进展. 中国病毒学, 17(3): 276-278.]Wan XB, Pang QF, Qiu FX, Xu AY, Zhang SQ. 1982. Experimental infection of human rotavirus of adult tree shrew[J]. J Med Res, 3: 2. [5] [万新邦, 庞其方, 丘福禧, 胥爱源, 张胜泉. 1982. 人轮状病毒实验感染成年树鼩的研究. 医学研究杂志, 3: 2.]Wan XB, Pang QF, Qiu FX, Zhang XS, Liu JC, Xv AY, Zhang SQ, Zhang HF, Xu WM..1985.Experimental studies on the susceptibility of adult Tupaia belangeri yunalis to human rotavirus[J].Chin J Microbiol Immunol,5:304-307.[万新邦, 庞其方, 丘福禧, 张新生, 刘玑昌, 胥爱源, 张胜泉, 张惠芬, 徐维民.1985.成年的中国云南树鼩对人轮状病毒易感性的实验研究. 中华微生物学和免疫学杂志, 5:304-307.]Xu XP, Chen HB, Ben KL. 2005. Application of tree shrew as an animal model in biomedical research[J]. Acta Lab Anim Sci Sin, 13(3): 187-190. [6] [徐新平, 陈红波, 贲昆龙. 2005. 树鼩在医学生物学中的应用. 中国实验动物学报, 13(3): 187-190.] [7]
-

计量
- 文章访问数: 2237
- HTML全文浏览量: 86
- PDF下载量: 2344
- 被引次数: 0