Preliminary investigation of viruses to the wild tree shrews (Tupaia belangeri chinese)
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摘要: 病毒学检测和监测是树鼩实验动物化和质量控制的重要标准和依据，而野生中缅树鼩是否携带人兽共患病毒鲜见报道。本研究采用酶联免疫吸附(ELISA)方法, 对来源于云南昆明市城郊青龙峡地区的野生树鼩是否携带单纯疱疹病毒、轮状病毒、流感病毒、柯萨奇病毒、甲肝病毒、乙肝病毒、丙肝病毒、丁肝病毒、登革热病毒、出血热病毒和麻疹病毒等11种常见病毒进行筛查。结果表明, 在已筛查的60只野生中缅树鼩中，可检测到单纯疱疹病毒和柯萨奇病毒，其血清抗体阳性比例分别为36.7% (22/60) 和1.67% (1/60)，而在粪便中仅检测到轮状病毒，其抗原阳性为6.7% (4/60), 未检测到其他病毒，初步显示了野生树鼩自然状态下携带病毒的状况。为此，建议将单纯疱疹病毒、柯萨奇病毒和轮状病毒列为普通级树鼩病毒质量控制的首检项目，进一步大样本筛查将显示是否将其他病毒列为必检项目。Abstract: Virological testing and monitoring is a fundamental part of quality control of experimental animals. However, there are few papers regarding the spectrum and status of natural infection in wild tree shrews with human and animal pathogenic viruses. Using enzyme-linked immunosorbent adsorption assay (ELISA), we tested sixty wild tree shrews captured from Qinglong, an outskirt region of Kunming, Yunnan Province, China for eleven viruses, including herpes simplex virus, coxsackie virus, influenza virus, HAV, HBV, HCV, HDV, dengue virus, hemorrhagic fever virus and measles virus. Our results showed that, in the serum samples, 22/60 (36.7%) and 1/60 (1.67%) were antibody positive for herpes simplex virus and coxsackie virus, respectively, and 4/60 (6.7%) were antigen positive for rotavirus in the feces. The remaining species of viruses were negative in these tree shrews. Based on these results, we propose that herpes simplex virus, coxsackie virus and cotavirus should be listed as top priority for routine virological monitoring of tree shrews.
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Key words:
- Tree shrew (Tupaia belangeri chinese) /
- Viruses /
- Virological monitoring
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