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Application of flow cytometry to detect ABO blood group antibody levels in rhesus monkeys and cynomolgus monkeys

ZHANG Chi WANG Xiao-Xiao WANG Lu XIANG Ying WEI Qing WANG Wan-Yu XIONG Yu-Lian

ZHANG Chi, WANG Xiao-Xiao, WANG Lu, XIANG Ying, WEI Qing, WANG Wan-Yu, XIONG Yu-Lian. Application of flow cytometry to detect ABO blood group antibody levels in rhesus monkeys and cynomolgus monkeys. Zoological Research, 2011, 32(1): 56-61. doi: 10.3724/SP.J.1141.2011.01056
Citation: ZHANG Chi, WANG Xiao-Xiao, WANG Lu, XIANG Ying, WEI Qing, WANG Wan-Yu, XIONG Yu-Lian. Application of flow cytometry to detect ABO blood group antibody levels in rhesus monkeys and cynomolgus monkeys. Zoological Research, 2011, 32(1): 56-61. doi: 10.3724/SP.J.1141.2011.01056

应用改良流式法检测猕猴和食蟹猴体内血型抗体水平的分布情况

doi: 10.3724/SP.J.1141.2011.01056
基金项目: 国家自然科学基金面上项目(30872384)
详细信息
    作者简介:

    张弛:张 弛

  • 中图分类号: R-332; Q959.848

Application of flow cytometry to detect ABO blood group antibody levels in rhesus monkeys and cynomolgus monkeys

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    Author Bio:

    ZHANG Chi

  • 摘要: 绝大部分灵长类动物存在与人类相似的ABO血型系统, 该研究采用改良流式法(flow cytometry method, FCM)检测猕猴及食蟹猴血清中血型抗体水平的分布情况。以流式细胞术为基础, 使用商品化人源红细胞为靶细胞, 并通过加入特异性荧光标记的抗人IgM或IgG二抗, 对收集的实验用猕猴及食蟹猴的血清样本进行检测, 以人类健康受试者的血清样本为对照, 比较两者血型抗体水平的差异。结果显示:预先用人O型浓缩红细胞吸附猴血清中所含种属间非特异性抗体后, FCM法能够准确检测其血型抗体水平及分型, 并且发现猴血清中天然血型抗体的水平明显低于健康人(P<0.05)。由此得出:通过预处理清除非特异性抗体的干扰后, FCM法同样适用于灵长类动物血清中血型抗体的检测, 也为构建灵长类动物模拟人ABO血型不合器官移植模型提供了技术保障和实验数据。
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    [J]. Eur J Immunol, 22(8): 2129-2133.Stussi G, Huggel K, Lutz HU, Schanz U, Rieben R, Seebach JD. 2005. Isotype-specific detection of ABO blood group antibodies using a novel flow cytometric method [J]. Br J Haematol, 130(6): 954-963.Tanabe K. 2007. Interinstitutional variation in the measurement of anti-A/B antibodies: the Japanese ABO-Incompatible Transplantation Committee survey [J]. Transplantation, 84(12 Suppl): S13-16.Terao K, Fujimoto K, Cho F, Honjo S. 1981.Detection of ABO group-specific substances on the red cells of the cynomolgus monkey [J]. Jpn J Med Sci Biol, 34(1): 1-7.Terao K, Hiyaoka A, Cho F, Honjo S. 1988. The simian-type M and the human-type ABO blood groups in the African green monkey (Cercopithecus aethiops): their inheritance, distribution and significance for the management of a breeding colony [J]. Lab Anim, 22(4): 347-354.
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出版历程
  • 收稿日期:  2010-12-08
  • 修回日期:  2010-12-30
  • 刊出日期:  2011-02-22

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