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Expression of cannabinoid and opioid receptors in nervous as well as immune systems of Macaca mulatta and Tupaia belangeri

ZHANG Qing-Yu FAN Xiao-Na CAO Yi

ZHANG Qing-Yu, FAN Xiao-Na, CAO Yi. Expression of cannabinoid and opioid receptors in nervous as well as immune systems of Macaca mulatta and Tupaia belangeri. Zoological Research, 2011, 32(1): 31-39. doi: 10.3724/SP.J.1141.2011.01031
Citation: ZHANG Qing-Yu, FAN Xiao-Na, CAO Yi. Expression of cannabinoid and opioid receptors in nervous as well as immune systems of Macaca mulatta and Tupaia belangeri. Zoological Research, 2011, 32(1): 31-39. doi: 10.3724/SP.J.1141.2011.01031

正常猕猴与树鼩神经系统和免疫系统组织中大麻素与阿片受体的表达

doi: 10.3724/SP.J.1141.2011.01031
基金项目: 中国科学院基础前沿研究专项项目(KSCX2-EW-J-23);中国科学院和云南省动物模型与人类疾病机理重点实验室资助项目
详细信息
    作者简介:

    张庆余, 男, 硕士研究生, 研究方向:毒品成瘾机理, E-mail:zqy0401@hotmail.com

    通讯作者:

    曹 毅

  • 中图分类号: Q959.832; Q959.842; R338.2; R971.3

Expression of cannabinoid and opioid receptors in nervous as well as immune systems of Macaca mulatta and Tupaia belangeri

More Information
    Author Bio:

    ZHANG Qing-Yu, E-mail:zqy0401@hotmail.com

    Corresponding author: CAO Yi
  • 摘要: 为应用猕猴和树鼩动物模型研究毒品成瘾对神经/免疫系统的影响提供基础数据,对大麻素及阿片受体在正常猕猴和树鼩神经系统和免疫系统的表达进行初步确定。采集正常猕猴和树鼩新鲜组织(皮质、小脑、脑干、海马、脊髓、脾脏), 应用半定量逆转录PCR和实时定量PCR的方法检测大麻素与阿片受体mRNA在猕猴和树鼩各组织中的表达情况。猕猴脑部各区包括脾脏均表达大麻素受体1 (CNR1), 而大麻素受体2 (CNR2)只表达于脾脏内。三类阿片受体中, mu(m)受体表达最为广泛, 在以上各组织中均有表达; delta (d) 受体表达的组织最少, 只在海马表达; kappa (k) 受体表达介于两者之间, 分别在皮质、小脑、脑干、脊髓中表达。在树鼩组织中, CNR1和CNR2表达于整个大脑重要脑区中, 且CNR1表达量高于同一区域内CNR2表达的量; 脾脏中CNR2的表达较高, 而CNR1不表达。三类阿片受体只有检测到μ受体在脑部与脾脏表达, 且在各个脑区的表达量明显高于脾脏的表达量; d受体和 k受体在被检各个组织中均无表达。总体而言, 两种大麻素受体在猕猴和树鼩体内表达情况与人类和鼠的情况类似, 而三类阿片受体在猕猴体内表达情况与人类更为接近。猕猴和树鼩可能可用于人类毒品成瘾的研究; 猕猴在某些神经受体的表达更接近人类, 其在研究毒品成瘾的机理和对免疫系统的影响方面仍有不可替代的地位。
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出版历程
  • 收稿日期:  2010-12-06
  • 修回日期:  2011-01-19
  • 刊出日期:  2011-02-22

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