留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China

CHEN Shi-Yi XU Ling Lü Long-Bao YAO Yong-Gang

CHEN Shi-Yi, XU Ling, Lü Long-Bao, YAO Yong-Gang. Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China. Zoological Research, 2011, 32(1): 17-23. doi: 10.3724/SP.J.1141.2011.01017
Citation: CHEN Shi-Yi, XU Ling, Lü Long-Bao, YAO Yong-Gang. Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China. Zoological Research, 2011, 32(1): 17-23. doi: 10.3724/SP.J.1141.2011.01017

昆明城郊中国树鼩群体线粒体DNA遗传多样性(英文)

doi: 10.3724/SP.J.1141.2011.01017
详细信息
    作者简介:

    陈仕毅

    通讯作者:

    姚永刚

  • 中图分类号: Q959.832; Q951.3; Q347

Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China

Funds: This study was supported by grants from Yunnan Province (2009CI119), the Chinese Academy of Sciences (KSCX2-EW-R-11 and KSCX2-EW-J-23) and the Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province
More Information
    Author Bio:

    CHEN Shi-Yi

    Corresponding author: YAO Yong-Gang
  • 摘要: 由于树鼩是灵长类动物的近亲, 且具有体型小、繁殖周期短、饲养管理成本低等优点, 长期以来被认为有望替代灵长类动物用于人类疾病的动物模型研究。然而, 目前对树鼩的群体遗传结构还知之甚少, 这极大地限制了其在疾病动物模型研究的应用, 也是其品系资源创制的瓶颈。本研究通过分析80只采自于云南省昆明周边地区的野生树鼩 (Tupaia belangeri chinensis) 线粒体DNA (mtDNA) 多态性, 结合国外报道的2个树鼩 (Tupaia belangeri) 序列比较后发现, 在604 bp的mtDNA控制区片段中共检测到29个核苷酸替代变异, 这些变异共界定了13种单倍型, 表现较高的群体遗传多样度。另外, 昆明地区的树鼩与国外报道的2个树鼩间存在较大的遗传分化, mtDNA控制区单倍型之间的核苷酸替换数大于18个, 远高于昆明地区树鼩群体内部不同单倍型之间的差异。选择含有代表性的mtDNA控制区单倍型的17个昆明地区树鼩个体进一步测定了细胞色素b基因片段 (1134 bp), 结合前人报道的数据分析, 结果进一步支持mtDNA控制区数据反映的遗传格局及揭示的昆明地区树鼩与国外报道树鼩之间的明显差异。本研究结果提示, 昆明地区树鼩与国外树鼩之间存在较大遗传差异, 在将树鼩用于人类疾病动物模型研究中要注意这些遗传差别。昆明城郊的树鼩群体具有较高的遗传多样度, 在开展近交系建立等工作时须考虑选取群体内部具有代表性的mtDNA世系。
  • [1] Alfonso J, Aguero F, Sanchez DO, Flugge G, Fuchs E, Frasch AC, Pollevick GD. 2004. Gene expression analysis in the hippocampal formation of tree shrews chronically treated with cortisol [J]. J Neurosci Res, 78(5): 702-710.Amako Y, Tsukiyama-Kohara K, Katsume A, Hirata Y, Sekiguchi S, Tobita Y, Hayashi Y, Hishima T, Funata N, Yonekawa H, Kohara M. 2010. Pathogenesis of hepatitis C virus infection in Tupaia belangeri [J]. J Virol, 84(1): 303-311.Arnason U, Adegoke JA, Bodin K, Born EW, Esa YB, Gullberg A, Nilsson M, Short RV, Xu XF, Janke A. 2002. Mammalian mitogenomic relationships and the root of the eutherian tree [J]. Proc Natl Acad Sci U S A, 99(12): 8151-8156.Bandelt H-J, Forster P, Röhl A. 1999. Median-joining networks for inferring intraspecific phylogenies [J]. Mol Biol Evol, 16(1): 37-48.Cao J, Yang EB, Su JJ, Li Y, Chow P. 2003. The tree shrews: adjuncts and alternatives to primates as models for biomedical research [J]. J Med Primatol, 32(3): 123-130.Fuchs E. 2005. Social stress in tree shrews as an animal model of depression: an example of a behavioral model of a CNS disorder [J]. CNS Spectr, 10(3): 182-190.Guindon S, Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood [J]. Syst Biol, 52(5): 696-704.He BL, Shen PQ, Chen LL, Jia JL, Liu RW, Li B, Zheng H, Li ML. 2009. Polymorphism microsatellites in tree shrews (Tupaia Belangeri Chinensis) [J]. Acta Lab Anim Sci Sin, 17(2): 143-145. (in Chinese) He DQ, Zhu Q, Chen SY, Wang HY, Liu YP, Yao YG. 2008. A homogenous nature of native Chinese duck matrilineal pool [J]. BMC Evol Biol, 8: 298.Helgen KM. 2005. Order Scandentia[M]// Wilson DE, Reeder DM (ed). 2005. Mammal Species of the World: A Taxonomic and Geographic Reference. 3rd ed. Maryland: Johns Hopkins University Press, 104-109.Jia T, Lin AQ, Wang R, Zhu WL, Xiao CH, Liu CY, Meng LH, Lian X, Wang ZK. 2009. Pilot study of Tupaia belangeri from Yunnan Province based on morphometrics of the skulls and mandibles [J]. Acta Theriol Sin, 29(3): 259-268. (in Chinese)Jia T, Yang XM, Li ZH, Zhu WL, Xiao CH, Liu CY, Wang ZK. 2008. Classified significance of Tupaia belangeri from Luquan District, Kunming based on Cyt b gene sequences [J]. Chn J Zool, 43(4): 26-33. (in Chinese)Köck J, Nassal M, MacNelly S, Baumert TF, Blum HE, von Weizsäcker F. 2001. Efficient infection of primary tupaia hepatocytes with purified human and woolly monkey hepatitis B virus [J]. J Virol, 75(11): 5084-5089.Li Y, Qin X, Cui J, Dai Z, Kang X, Yue H, Zhang Y, Su J, Cao J, Ou C, Yang C, Duan X, Yue H, Liu Y. 2008. Proteome analysis of aflatoxin B1-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) and functional identification of candidate protein peroxiredoxin II [J]. Proteomics, 8(7): 1490-1501.Librado P, Rozas J. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data [J]. Bioinformatics, 25(11): 1451-1452.Murphy WJ, Eizirik E, O'Brien SJ, Madsen O, Scally M, Douady CJ, Teeling E, Ryder OA, Stanhope MJ, de Jong WW, Springer MS. 2001. Resolution of the early placental mammal radiation using Bayesian phylogenetics [J]. Science, 294(5550): 2348-2351.Nei M. 1987. Molecular Evolutionary Genetics [M]. New York: Columbia University Press.Nie W, Fu B, O'Brien PC, Wang J, Su W, Tanomtong A, Volobouev V, Ferguson-Smith MA, Yang F. 2008. Flying lemurs—the 'flying tree shrews'? Molecular cytogenetic evidence for a Scandentia-Dermoptera sister clade [J]. BMC Biol, 6: 18.Norton TT, Amedo AO, Siegwart JT, Jr. 2006. Darkness causes myopia in visually experienced tree shrews [J]. Invest Ophthalmol Vis Sci, 47(11): 4700-4707.Olson LE, Sargis EJ, Martin RD. 2005. Intraordinal phylogenetics of treeshrews (Mammalia : Scandentia) based on evidence from the mitochondrial 12S rRNA gene [J]. Mol Phylogenet Evol, 35(3): 656-673.Peng YZ, Ye ZZ, Zou RJ, Wang YX, Tian BP, Ma YY, Shi LM. 1991. Biology of Chinese tree shrews (Tupaia belangeri chinensis) [M]. Kunming: Yunnan Science and Technology Press. (in Chinese)Previc FH. 2009. The Dopaminergic mind in human evolution and history [M]. Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, São Paulo, Delhi: Cambridge University Press.Ren S, Nassal M. 2001. Hepatitis B virus (HBV) virion and covalently closed circular DNA formation in primary tupaia hepatocytes and human hepatoma cell lines upon HBV genome transduction with replication-defective adenovirus vectors
    [J]. J Virol, 75(3):     1104-1116.Sargis EJ. 2004. New views on tree shrews: the role of tupaiids in primate supraordinal relationships [J]. Evol Anthropol, 13(2): 56-66.Schmitz J, Ohme M, Zischler H. 2000. The complete mitochondrial genome of Tupaia belangeri and the phylogenetic affiliation of Scandentia to other eutherian orders [J]. Mol Biol Evol, 17(9): 1334-1343.Seiffert ER, Simons EL, Attia Y. 2003. Fossil evidence for an ancient divergence of lorises and galagos [J]. Nature, 422(6930): 421-424.Su JJ. 1987. Experimental infection of human hepatitis B virus (HBV) in adult tree shrews [J]. Chn J Pathol, 16(2): 103-106, 122. (in Chinese)Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 [J]. Mol Biol Evol, 24(8): 1596-1599.Wang YX. 1987. Taxonomic research on Burma-Chinese tree shrew, Tupaia belangeri (Wagner), from Southern China [J]. Chn J Zool, 8(3): 213-230. (in Chinese)Xu X, Chen H, Cao X, Ben K. 2007. Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma [J]. J Gen Virol, 88: 2504-2512.Yan RQ, Su JJ, Huang DR, Gan YC, Yang C, Huang GH. 1996. Human hepatitis B virus and hepatocellular carcinoma. I. Experimental infection of tree shrews with hepatitis B virus [J]. J Cancer Res Clin Oncol, 122: 283-288.
    [3]
     
  • [1] Zhong-Yuan Shen, Dan Yu, Xin Gao, Fu-Tie Zhang, Huan-Zhang Liu.  Genetic diversity and reproductive success of a wild population of Chinese sturgeon (Acipenser sinensis) from the Yangtze River inferred from juveniles born in 2014, Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.011
    [2] Nikolay A. Poyarkov, Jr., Chatmongkon Suwannapoom, Parinya Pawangkhanant, Akrachai Aksornneam, Tang Van Duong, Dmitriy V. Korost, Jing Che.  A new genus and three new species of miniaturized microhylid frogs from Indochina (Amphibia: Anura: Microhylidae: Asterophryinae), Zoological Research. doi: 10.24272/j.issn.2095-8137.2018.019
    [3] Jin-Jin MIN, Rong-Hui YE, Gen-Fang ZHANG, Rong-Quan ZHENG.  Microsatellite analysis of genetic diversity and population structure of freshwater mussel (Lamprotula leai), Zoological Research. doi: 10.13918/j.issn.2095-8137.2015.1.34
    [4] Ya HE, Zheng-Huan WANG, Xiao-Ming WANG.  Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation, Zoological Research. doi: 10.13918/j.issn.2095-8137.2014.6.528
    [5] ZHENG Wen-Juan, LAI Yu-Hong, YOU Xin-Yu, QIN Xi-Han, ZHU Shi-Hua.  Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences, Zoological Research. doi: 10.3724/SP.J.1141.2012.03329
    [6] Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI.  Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis, Zoological Research. doi: 10.3724/SP.J.1141.2012.E03-04E33
    [7] SHAN Wen-Juan, LIU Jiang, Mahmut HALIK.  Genetic structure and subspecies divergence of Lepus capensis in Xinjiang, Zoological Research. doi: 10.3724/SP.J.1141.2011.02179
    [8] QI De-lin.  Genetic Variation and Diversity of Gymncypris eckloni in the Upper Yellow River Inferred from Mitochondrial Cytochrome b Gene, Zoological Research. doi: 10.3724/SP.J.1141.2009.03255
    [9] LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu.  Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers, Zoological Research.
    [10] YAN Liang, ZHANG Yan, WANG Ning, ZHANG Li, NIE Liu-wang.  Comparison of Mitochondrial Control Region Sequences Between Chelydridae and Platysternidae, Zoological Research. doi: 10.3724/SP.J.1141.2008.02127
    [11] YANG Bo, CHEN Xiao-yong, YANG Jun-xing.  Structure of the Mitochondrial DNA Control Region and Population Genetic Diversity Analysis of Anabarilius grahami (Regan), Zoological Research. doi: 10.3724/SP.J.1141.2008.04379
    [12] ZHU Shi-hua, ZHENG Wen-juan, ZOU Ji-xing, YANG Ying-chun, SHEN Xi-quan.  Mitochondrial DNA Control Region Structure and MolecularPhylogenetic Relationship of Carangidae, Zoological Research.
    [13] SHI Hong , LI Yi , LUO Hong, JI Xue-ping, SU Bing , *The Genetic Study on the Patrilineal and Matrilineal Origin of the Bo People in Yunnan, Zoological Research.
    [14] TAO Feng-yong, WANG Xiao-ming, ZHENG He-xun, FANG Sheng-guo.  Genetic Structure and Geographic Subdivision of Four Populations of the Chinese Giant Salamander (Andrias davidianus), Zoological Research.
    [15] LI Qing-qing, ZHANG Ya-ping.  A Molecular Phylogeny of Macaca Based on Mitochondrial Control Region Sequences, Zoological Research.
    [16] JIANG Jian-Ping, ZHOU Kai-Ya.  Phylogenetic Relationships of Chinese Brown Frogs Inferred from Mitochondrial DNA Sequence of 12 S Ribosome RNA Gene, Zoological Research.
    [17] YAO Yong-Gang, KONG Qing-Peng, ZHANG Ya-Ping.  Methods and Thoughts on Detecting Nucleotide Variations in Human Mitochondrial DNA, Zoological Research.
    [18] ZHANG Si-ming, DENG Huai, WEI Qi-wei, WANG Deng-qiang, WU Qing-jiang.  The Preliminary Evidence For Low Genetic Diversity in Chinese Sturgeon (Acipenser sinensis) Revealed By Protein Electrophoresis, Zoological Research.
    [19] LI Dian-xiang, LI Chuan-yin, RONG Qian, WANG Jin-xing.  A Map of Restriction of mtDNA From Misgurnus anguillicaudatus, Zoological Research.
    [20] HE Zheng-quan, ZHANG Ya-ping, JIAN Chen-song, ZHU Weng-shi, YU Yin-huai et al..  Study on mtDNA Rflp of Guiznou Yellow Cattle Breeds, Zoological Research.
  • 加载中
计量
  • 文章访问数:  2430
  • HTML全文浏览量:  33
  • PDF下载量:  2384
  • 被引次数: 0
出版历程
  • 收稿日期:  2010-12-02
  • 修回日期:  2010-12-27
  • 刊出日期:  2011-02-22

目录

    /

    返回文章
    返回