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Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

LI Yi-Jiang GAO Yue-Dong GUO Yan LU Cai-Xia HUANG Jing-Fei XIA Xue-Shan DA

LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483
Citation: LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483

树鼩CD3ε全长编码序列的克隆及分子特征分析

doi: 10.3724/SP.J.1141.2010.05483
基金项目: 国家重点基础研究发展计划(973计划)分课题(2007CB512807);云南省科技基础条件平台建设计划项目(2006PT07-2);国家科技支撑计划项目(2009BAI83B02)
详细信息
    作者简介:

    李乙江,硕士研究生,E-mail: liyijiang88.student@sina.com

  • 中图分类号: Q959.83; Q951.3; Q75

Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

More Information
    Author Bio:

    LI Yi-Jiang,E-mail: liyijiang88.student@sina.com

  • 摘要: 树鼩可作为人类疾病的良好模型,但缺乏对其免疫功能研究的基本标志和单克隆抗体。该研究应用RT-PCR技术,分别从3只树鼩的肝脏、脾脏和血液中克隆了CD 3ε全长编码序列并用Discovery Studio等软件对其分子特征进行了分析。结果显示,树鼩CD3ε cDNA的开放阅读框全长582 bp,编码193个氨基酸。树鼩与其他哺乳动物的CD3ε蛋白整体结构近似,与人和恒河猴CD3ε的亲缘关系较近,胞内域与跨膜域高度保守,但胞外域出现2个潜在的糖基化位点,表面电荷亦存在差异。该研究为今后制备树鼩CD3单克隆抗体及功能研究奠定初步的基础。
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出版历程
  • 收稿日期:  2010-01-22
  • 修回日期:  2010-09-15
  • 刊出日期:  2010-10-22

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