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Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica

ZHANG Ying YU Jian-Feng YANG Li WANG Xing-Guo GU Zhi-Liang

ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476
Citation: ZHANG Ying, YU Jian-Feng, YANG Li, WANG Xing-Guo, GU Zhi-Liang. Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica. Zoological Research, 2010, 31(5): 476-482. doi: 10.3724/SP.J.1141.2010.05476

家鸽Caveolin-1基因全长cDNA的克隆、序列和组织表达分析

doi: 10.3724/SP.J.1141.2010.05476
基金项目: 江苏省“六大人才高峰” 第六批高层次人才项目;江苏省自然科学基金(BK2005026)
详细信息
    作者简介:

    张营(1984 — ),男,山东淄博人,硕士研究生,主要从事动物分子遗传学研究;E-mail: yingzhang0928@yahoo.cn

    通讯作者:

    顾志良,博士,教授

  • 中图分类号: Q959.725; Q78; Q951.3; Q341

Cloning and characterization of Caveolin-1 gene in pigeon,Columba livia domestica

More Information
    Author Bio:

    ZHANG Ying;E-mail: yingzhang0928@yahoo.cn

    Corresponding author: GU Zhi-Liang
  • 摘要: 窖蛋白(Caveolin)是一类构成细胞膜上胞膜窖主要结构的标志蛋白,由Caveolin基因家族编码而成。Caveolin-1基因是Caveolin基因家族的成员之一。该实验采用RT-PCR与RACE(rapid amplification of cDNA ends)技术成功地克隆了家鸽Caveolin-1基因的全长cDNA。该cDNA全长2 605 bp,包含537 bp的完整编码区,编码178个氨基酸;分析发现家鸽Caveolin-1基因编码区与牛、家犬、鸡、褐家鼠等核苷酸同源性为80.1%~93.4%,氨基酸同源性高达85.4%~97.2%;半定量RT-PCR分析表明该基因在家鸽各种组织广泛表达,脂肪中表达量最高,各种肌肉中表达量次之,肝脏中表达量最低。此结果说明家鸽Caveolin-1基因可能与脂肪、肌肉中的某些代谢途径有关。
  • [1] Cohen AW, Park DS, Woodman SE, Williams TM, Chandra M, Shirani J, Pereira de Souza A, Kitsis RN, Russell RG, Weiss LM, Tang B, Jelicks LA, Factor SM, Shtutin V, Tanowitz HB, Lisanti MP. 2003. Caveolin-1 null mice develop cardiac hypertrophy with hyperactivation of p42/44 MAP kinase in cardiac fibroblasts [J]. Am J Physiol Cell Physiol, 284(2): 457-474.Cohen AW, Razani B, Wang XB, Combs TP, Williams TM, Scherer PE, Lisanti MP. 2003. Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue [J]. Am J Physiol Cell Physiol, 285(1): 222-235.Engelman JA, Zhang XL, Lisanti MP. 1998. Genes encoding human caveolin-1 and -2 are co-localized to the D7S522 locus (7q31.1), a known fragile site (FRA7G) that is frequently deleted in human cancers [J]. FEBS Lett, 436(3): 403-410.Fanzani A, Musaro A, Stoppani E, Giuliani R, Colombo F, Preti A, Marchesini S. 2007. Hypertrophy and atrophy inversely regulate caveolin-3 expression in myoblasts [J]. Biochem Biophys Res Commun, 357(1): 314-318.Hailstones D, Sleer LS, Parton RG, Stanley KK. 1998. Regulation of caveolin and caveolae by cholesterol in MDCK cells [J]. J Lipid Res, 39(2): 369-379.Jasmin JF, Malhotra S, Singh Dhallu M, Mercier I, Rosenbaum DM, Lisanti MP. 2007. Caveolin-1 deficiency increases cerebral ischemic injury [J]. Circ Res, 100(5): 721-729.Le Lay S, Blouin CM, Hajduch E, Dugail I. 2009. Filling up adipocytes with lipids: Lessons from caveolin-1 deficiency [J]. Biochim. Biophys Acta, 1791(6): 514-518.Lu X, Zhang Y, Wang X, Zhang Y, Xu J, Lai R, Gu Z. 2010. Identification and characterization of myostatin gene in Rough-skinned sculp, Trachidermus fasciatus[J]. Zool Res, 31(4): 387-394. [卢祥云,张  营,王星果,张燕萍,徐建荣,赖 仭,顾志良. 2010. 松江鲈(Trachidermus fasciatus肌肉生长抑制素基因克隆、序列和表达特征分析. 动物学研究,31(4): 387-394.])Parton RG, Hanzal-Bayer M, Hancock JF. 2006. Hancock. Biogenesis of caveolae: A structural model for caveolin-induced domain formation[J]. J Cell Sci, 119: 787-796.Pol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG. 2001. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance [J]. J Cell Biol, 152(5): 1057-1070.Pol A, Martin S, Fernandez MA, Ferguson C, Carozzi A, Luetterforst R, Enrich C, Parton RG. 2004. Dynamic and regulated association of caveolin with lipid bodies: modulation of lipid body motility and function by a dominant negative mutant [J]. Mol Biol cell, 15(1): 99-110.Scherer PE, Lewis RY, Volonte D, Engelman JA, Galbiati F, Couet J, Kohtz DS, van Donselaar E, Peters P, Lisanti MP. 1997. Cell-type and tissue-specific expression of caveolin-2: Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo [J]. J Biol Chem, 272(46): 29337-29346.Smythe GM, Eby JC, Disatnik MH. 2003. A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes [J]. J Cell Sci, 116: 4739-4749., Rando TASonnino S, Prinetti A. 2009. Sphingolipids and membrane environments for caveolin [J]. FEBS Lett, 583(4): 597-606.Wang C, Mei YJ, Li L, Mo DL, Li JQ, Zhang H, Tian XG,Chen YS.2008. Molecular characterization and expression analysis of caveolin-1 in pig tissues
    [J]. Sci Chn Ser C: Life Sci, 51(7) : 655-661.Yamada E. 1955. The fine structures of the gall bladder epithelium of the mouse [J]. J Biophys Biochem Cytol,1(5): 445-458.Zhu Z, Li Y, Mo D, Li K, Zhao S. 2006. Molecular characterization and expression analysis of the porcine Caveolin-3 gene [J]. Biochem Biophys Res Commun, 346(1): 7-13.Cohen AW, Park DS, Woodman SE, Williams TM, Chandra M, Shirani J, Pereira de Souza A, Kitsis RN, Russell RG, Weiss LM, Tang B, Jelicks LA, Factor SM, Shtutin V, Tanowitz HB, Lisanti MP. 2003. Caveolin-1 null mice develop cardiac hypertrophy with hyperactivation of p42/44 MAP kinase in cardiac fibroblasts [J]. Am J Physiol Cell Physiol, 284(2): 457-474.Cohen AW, Razani B, Wang XB, Combs TP, Williams TM, Scherer PE, Lisanti MP. 2003. Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue [J]. Am J Physiol Cell Physiol, 285(1): 222-235.Engelman JA, Zhang XL, Lisanti MP. 1998. Genes encoding human caveolin-1 and -2 are co-localized to the D7S522 locus (7q31.1), a known fragile site (FRA7G) that is frequently deleted in human cancers [J]. FEBS Lett, 436(3): 403-410.Fanzani A, Musaro A, Stoppani E, Giuliani R, Colombo F, Preti A, Marchesini S. 2007. Hypertrophy and atrophy inversely regulate caveolin-3 expression in myoblasts [J]. Biochem Biophys Res Commun, 357(1): 314-318.Hailstones D, Sleer LS, Parton RG, Stanley KK. 1998. Regulation of caveolin and caveolae by cholesterol in MDCK cells [J]. J Lipid Res, 39(2): 369-379.Jasmin JF, Malhotra S, Singh Dhallu M, Mercier I, Rosenbaum DM, Lisanti MP. 2007. Caveolin-1 deficiency increases cerebral ischemic injury [J]. Circ Res, 100(5): 721-729.Le Lay S, Blouin CM, Hajduch E, Dugail I. 2009. Filling up adipocytes with lipids: Lessons from caveolin-1 deficiency [J]. Biochim. Biophys Acta, 1791(6): 514-518.Lu X, Zhang Y, Wang X, Zhang Y, Xu J, Lai R, Gu Z. 2010. Identification and characterization of myostatin gene in Rough-skinned sculp, Trachidermus fasciatus[J]. Zool Res, 31(4): 387-394. [卢祥云,张  营,王星果,张燕萍,徐建荣,赖 仭,顾志良. 2010. 松江鲈(Trachidermus fasciatus肌肉生长抑制素基因克隆、序列和表达特征分析. 动物学研究,31(4): 387-394.])Parton RG, Hanzal-Bayer M, Hancock JF. 2006. Hancock. Biogenesis of caveolae: A structural model for caveolin-induced domain formation
    [J]. J Cell Sci, 119: 787-796.Pol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG. 2001. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance[J]. J Cell Biol, 152(5): 1057-1070.Pol A, Martin S, Fernandez MA, Ferguson C, Carozzi A, Luetterforst R, Enrich C, Parton RG. 2004. Dynamic and regulated association of caveolin with lipid bodies: modulation of lipid body motility and function by a dominant negative mutant[J]. Mol Biol cell, 15(1): 99-110.Scherer PE, Lewis RY, Volonte D, Engelman JA, Galbiati F, Couet J, Kohtz DS, van Donselaar E, Peters P, Lisanti MP. 1997. Cell-type and tissue-specific expression of caveolin-2: Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric complex in vivo [J]. J Biol Chem, 272(46): 29337-29346.Smythe GM, Eby JC, Disatnik MH. 2003. A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes [J]. J Cell Sci, 116: 4739-4749., Rando TASonnino S, Prinetti A. 2009. Sphingolipids and membrane environments for caveolin [J]. FEBS Lett, 583(4): 597-606.Wang C, Mei YJ, Li L, Mo DL, Li JQ, Zhang H, Tian XG,Chen YS.2008. Molecular characterization and expression analysis of caveolin-1 in pig tissues
    [J]. Sci Chn Ser C: Life Sci, 51(7) : 655-661.Yamada E. 1955. The fine structures of the gall bladder epithelium of the mouse [J]. J Biophys Biochem Cytol,1(5): 445-458.Zhu Z, Li Y, Mo D, Li K, Zhao S. 2006. Molecular characterization and expression analysis of the porcine Caveolin-3 gene [J]. Biochem Biophys Res Commun, 346(1): 7-13. 
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  • 收稿日期:  2010-02-08
  • 修回日期:  2010-09-09
  • 刊出日期:  2010-10-22

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