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Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish

ZHAO Cai-Lian YANG Qi-Wen HU Jia-Rui YE Ding GONG Wu-Ming Lü Hai-Ying XU Z

ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469
Citation: ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 2010, 31(5): 469-475. doi: 10.3724/SP.J.1141.2010.05469

在斑马鱼Ⅰ和Ⅱ期卵母细胞中优势表达基因zRAP55的鉴定(英文)

doi: 10.3724/SP.J.1141.2010.05469
基金项目: 国家基础研究项目(2004CB117400);国家自然科学基金(30150005;30270675)
详细信息
    作者简介:

    赵彩莲,E-mail:cailian305@yahoo.com.cn

  • 中图分类号: Q959.468;Q133;Q786

Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish

Funds: This investigation was supported by grants from the National Basic Research Program of China (2004CB117400); the National Natural Science Foundation of China (30150005; 30270675)
More Information
    Author Bio:

    ZHAO Cai-Lian,E-mail:cailian305@yahoo.com.cn

  • 摘要: 通过性腺特异性表达基因的筛选,作者发现了一个在斑马鱼卵巢中富集的基因zRAP55。zRAP55蛋白由382个氨基酸组成并含有一个高度保守的Lsm区。zRAP55蛋白与其他脊椎动物一致性在56%以上。RT-PCR结果表明,zRAP55优势表达于卵巢中。原位杂交和免疫组织化学结果表明:在Ⅰ期和Ⅱ期卵母细胞中,zRAP55的阳性信号强烈,均匀地分布于整个细胞质中,但是在Ⅲ期和Ⅳ期卵母细胞中均检测不到信号。作为一个RNA相关蛋白,zRAP55可能在早期卵母细胞中具有调节蛋白质翻译的重要作用。
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出版历程
  • 收稿日期:  2010-03-15
  • 修回日期:  2010-08-11
  • 刊出日期:  2010-10-22

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