留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

Application of the genome editing tool CRISPR/Cas9 in non-human primates

Xin LUO Min LI Bing SU

Xin LUO, Min LI, Bing SU. Application of the genome editing tool CRISPR/Cas9 in non-human primates. Zoological Research, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214
Citation: Xin LUO, Min LI, Bing SU. Application of the genome editing tool CRISPR/Cas9 in non-human primates. Zoological Research, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214

CRISPR/Cas9基因组编辑技术在非人灵长类中的应用

doi: 10.13918/j.issn.2095-8137.2016.4.214
详细信息
    通讯作者:

    Bing SU

Application of the genome editing tool CRISPR/Cas9 in non-human primates

Funds: the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13010000) and the National Natural Science Foundation of China (31130051)
More Information
    Corresponding author: Bing SU
  • 摘要: 近年来,CRISPR/Cas9基因编辑技术已经成功在多种实验动物中实现基因编辑。与小鼠等其他模式动物相比,非人灵长类在解剖、生理、行为和遗传上都与人类更接近。因此,非人灵长类在研究人类进化、人类认知功能、人类神经退行性疾病及药物筛选上具有巨大的优势,是理想的动物模型。目前,利用CRISPR/Cas9在非人灵长类基因编辑方面已经取得了一些进展,但是还存在很多问题。本文较为系统地总结了CRISPR/Cas9以及衍生的paCas9技术在非人灵长类基因编辑应用方面的进展,存在的问题以及改进的方向。
  • [1] Aida T, Chiyo K, Usami T, Ishikubo H, Imahashi R, Wada Y, Tanaka KF, Sakuma T, Yamamoto T, Tanaka K. 2015. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Genome Biology, 16: 87.
    [2] Belmonte JCI, Callaway EM, Caddick SJ, Churchland P, Feng G, Homanics GE, Lee KF, Leopold DA, Miller CT, Mitchell JF, Mitalipov S, Moutri AR, Movshon JA, Okano H, Reynolds JH, Ringach D, Sejnowski TJ, Silva AC, Strick PL, Wu J, Zhang F. 2015. Brains, genes, and primates. Neuron, 86(3): 617-631.
    [3] Betizeau M, Cortay V, Patti D, Pfister S, Gautier E, Bellemin-Ménard A, Afanassieff M, Huissoud C, Douglas RJ, Kennedy H, Dehay C. 2013. Precursor diversity and complexity of lineage relationships in the outer subventricular zone of the primate. Neuron, 80(2): 442-457.
    [4] Blakemore C, Clark JM, Nevalainen T, Oberdorfer M, Sussman A. 2012. Implementing the 3Rs in neuroscience research: a reasoned approach. Neuron, 75(6): 948-950.
    [5] Brouns SJJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJH, Snijders APL, Dickman MJ, Makarova KS, Koonin EV, van der Oost J. 2008. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science, 321(5891): 960-964.
    [6] Caplan AL, Parent B, Shen M, Plunkett C. 2015. No time to waste-the ethical challenges created by CRISPR: CRISPR/Cas, being an efficient, simple, and cheap technology to edit the genome of any organism, raises many ethical and regulatory issues beyond the use to manipulate human germ line cells. EMBO Reports, 16(11): 1421-1426.
    [7] Chan AWS, Chong KY, Martinovich C, Simerly C, Schatten G. 2001. Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science, 291(5502): 309-312.
    [8] Chen YC, Zheng YH, Kang Y, Yang WL, Niu YY, Guo XY, Tu ZC, Si CY, Wang H, Xing RX, Pu XQ, Yang SH, Li SH, Ji WZ, Li XJ. 2015. Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9. Human Molecular Genetics, 24(13): 3764-3774.
    [9] Cho SW, Kim S, Kim Y, Kweon J, Kim HS, Bae S, Kim JS. 2014. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Research, 24(1): 132-141.
    [10] Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kühn R. 2015. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nature Biotechnology, 33(5): 543-548.
    [11] Cong L, Ran FA, Cox D, Lin SL, Barretto R, Habib N, Hsu PD, Wu XB, Jiang WY, Marraffini LA, Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science, 339(6121): 819-823.
    [12] Cyranoski D. 2016. Monkey kingdom-China is positioning itself as a world leader in primate research. Nature, 532(7599): 300-302.
    [13] Doudna JA, Charpentier E. 2014. The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213): 1258096.
    [14] Fu YF, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nature Biotechnology, 32(3): 279-284.
    [15] Guo XY, Li XJ. 2015. Targeted genome editing in primate embryos. Cell Research, 25(7): 767-768.
    [16] Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn L, Porteus MH. 2015. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotechnology, 33(9): 985-989.
    [17] Holdren JP, Shelanski H, Vetter D, Goldfuss C. 2015. Improving transparency and ensuring continued safety in biotechnology. Office of Science and Technology Policy.
    [18] Kawano F, Suzuki H, Furuya A, Sato M. 2015. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nature Communications, 6: 6256.
    [19] Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. 2010. Rapid blue-light-mediated induction of protein interactions in living cells. Nature Methods, 7(12): 973-975.
    [20] Kim S, Kim D, Cho SW, Kim J, Kim JS. 2014. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Research, 24(6): 1012-1019.
    [21] Konermann S, Brigham MD, Trevino AE, Hsu PD, Heidenreich M, Cong L, Platt RJ, Scott DA, Church GM, Zhang F. 2013. Optical control of mammalian endogenous transcription and epigenetic states. Nature, 500(7463): 472-476.
    [22] Koo T, Lee J, Kim JS. 2015. Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9. Molecules and Cells, 38(6): 475-481.
    [23] Lanphier E, Urnov F, Haecker SE, Werner M, Smolenski J. 2015. Don’t edit the human germ line. Nature, 519(7544): 410-411.
    [24] Liang PP, Xu YW, Zhang XY, Ding CH, Huang R, Zhang Z, Lv J, Xie XW, Chen YX, Li YJ, Sun Y, Bai YF, Zhou SY, Ma WB, Zhou CQ, Huang JJ. 2015. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & Cell, 6(5): 363-372.
    [25] Liu HT, Yu XH, Li KW, Klejnot J, Yang HY, Lisiero D, Lin CT. 2008. Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science, 322(5907): 1535-1539.
    [26] Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. 2015. Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nature Biotechnology, 33(5): 538-542.
    [27] Nihongaki Y, Kawano F, Nakajima T, Sato M. 2015a. Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nature Biotechnology, 33(7): 755-760.
    [28] Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M. 2015b. CRISPR-Cas9-based photoactivatable transcription system. Chemistry & Biology, 22(2): 169-174.
    [29] Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. 2014. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell, 156(5): 935-949.
    [30] Niu YY, Shen B, Cui YQ, Chen YC, Wang JY, Wang L, Kang Y, Zhao XY, Si W, Li W, Xiang AP, Zhou JK, Guo XJ, Bi Y, Si CY, Hu B, Dong GY, Wang H, Zhou ZM, Li TQ, Tan T, Pu XQ, Wang F, Ji SH, Zhou Q, Huang XX, Ji WZ, Sha JH. 2014. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell, 156(4): 836-843.
    [31] Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. 2013. RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nature Methods, 10(10): 973-976.
    [32] Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H. 2014. Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Research, 24(6): 1020-1027.
    [33] Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. 2013. Genome engineering using the CRISPR-Cas9 system. Nature Protocols, 8(11): 2281-2308.
    [34] Renaud JB, Boix C, Charpentier M, De Cian A, Cochennec J, Duvernois-Berthet E, Perrouault L, Tesson L, Edouard J, Thinard R, Cherifi Y, Menoret S, Fontaniere S, de Crozé N, Fraichard A, Sohm F, Anegon I, Concordet JP, Giovannangeli C. 2016. Improved genome editing efficiency and flexibility using modified oligonucleotides with TALEN and CRISPR-Cas9 nucleases. Cell Reports, 14(9): 2263-2272.
    [35] Sander JD, Joung JK. 2014. CRISPR-Cas systems for editing, regulating and targeting genomes. Nature Biotechnology, 32(4): 347-355.
    [36] Sung YH, Kim JM, Kim HT, Lee J, Jeon J, Jin Y, Choi JH, Ban YH, Ha SJ, Kim CH, Lee HW, Kim JS. 2014. Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases. Genome Research, 24(1): 125-131.
    [37] Wan HF, Feng CJ, Teng F, Yang SH, Hu BY, Niu YY, Xiang AP, Fang WZ, Ji WZ, Li W, Zhao XY, Zhou Q. 2015. One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system. Cell Research, 25(2): 258-261.
    [38] Wang HY, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. 2013. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell, 153(4): 910-918.
    [39] Yang H, Wang HY, Shivalila CS, Cheng AW, Shi LY, Jaenisch R. 2013. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell, 154(6): 1370-1379.
    [40] Yin H, Xue W, Chen SD, Bogorad RL, Benedetti E, Grompe M, Koteliansky V, Sharp PA, Jacks T, Anderson DG. 2014. Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype. Nature Biotechnology, 32(6): 551-553.
    [41] Yu C, Liu YX, Ma TH, Liu K, Xu SH, Zhang Y, Liu HL, Russa ML, Xie M, Ding S, Qi LS. 2015. Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell, 16(2): 142-147.
    [42] Zhang XL, Pang W, Hu XT, Li JL, Yao YG, Zheng YT. 2014. Experimental primates and non-human primate (NHP) models of human diseases in China: current status and progress. Zoological Research, 2014, 35(6): 447-464.
    [43] Zhao P, Zhang Z, Lv XY, Zhao X, Suehiro SJ, Jiang YN, Wang XQ, Mitani SH, Gong HP, Xue D. 2016. One-step homozygosity in precise gene editing by an improved CRISPR/Cas9 system. Cell Research, 26(5): 633-636.
    [44] Zhou XY, Wang LL, Du Y, Xie F, Li L, Liu Y, Liu CH, Wang SQ, Zhang SB, Huang XX, Wang Y, Wei H. 2016. Efficient generation of gene modified pigs harboring precise orthologous human mutation via CRISPR/Cas9-induced homology-directed repair in zygotes. Human Mutation, 37(1): 110-118.
    [45] Zuris JA, Thompson DB, Shu YL, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR. 2015. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nature Biotechnology, 33(1): 73-80.
  • [1] Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng.  Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research, Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.049
    [2] Bo Zhang, Zhi-Gang Zhou, Yin Zhou, Yong-Chang Chen.  Increased attention to snake images in cynomolgus monkeys: an eye-tracking study, Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.005
    [3] Dong-Dong Qin, Shu-Fei Feng, Fei-Yu Zhang, Na Wang, Wen-Jie Sun, Yin Zhou, Teng-Fang Xiong, Xian-Lai Xu, Xiao-Ting Yang, Xiang Zhang, Xue Zhu, Xin-Tian Hu, Lei Xiong, Yun Liu, Yong-Chang Chen.  Potential use of actigraphy to measure sleep in monkeys: comparison with behavioral analysis from videography, Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.056
    [4] Zhen-Zhen Chen, Yu-Yu Niu.  Stem cell therapy for Parkinson’s disease using non-human primate models, Zoological Research. doi: 10.24272/j.issn.2095-8137.2019.053
    [5] Xun Ma, Avery Sum-Yu Wong, Hei-Yin Tam, Samuel Yung-Kin Tsui, Dittman Lai-Shun Chung, Bo Feng.  In vivo genome editing thrives with diversified CRISPR technologies, Zoological Research. doi: 10.24272/j.issn.2095-8137.2017.012
    [6] Jia-Li LI, Yong-Tang ZHENG, Xu-Dong ZHAO, Xin-Tian HU.  Meeting report: the 4th symposium on animal models of non-human primates in Kunming, Yunnan, China, Zoological Research. doi: 10.13918/j.issn.2095-8137.2016.6.361
    [7] Li-Fang JIN, Jin-Song LI.  Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems, Zoological Research. doi: 10.13918/j.issn.2095-8137.2016.4.205
    [8] Lei CHEN, Gui WANG, Ya-Nan ZHU, Hui XIANG, Wen WANG.  Advances and perspectives in the application of CRISPR/Cas9 in insects, Zoological Research. doi: 10.13918/j.issn.2095-8137.2016.4.220
    [9] Ming SHAO, Tian-Rui XU, Ce-Shi CHEN.  The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models, Zoological Research. doi: 10.13918/j.issn.2095-8137.2016.4.191
    [10] Xiao-Liang ZHANG, Wei PANG, Xin-Tian HU, Jia-Li LI, Yong-Gang YAO, Yong-Tang ZHENG.  Experimental primates and non-human primate (NHP) models of human diseases in China: current status and progress, Zoological Research. doi: 10.13918/j.issn.2095-8137.2014.6.447
    [11] Peng-Lai FAN, Zuo-Fu XIANG.  Ecotourism disturbances to non-human primates, Zoological Research. doi: 10.3724/SP.J.1141.2013.01055
    [12] Special Issue for Primates and Animal Models of Human Diseases, Zoological Research.
    [13] ZHANG Jing, QI Xiao-Guang, ZHANG Kan, ZHANG Pei, GUO Song-Tao, WEI Wei, LI Bao-Guo.  Diversity and development of positional behavior in non-human primates, Zoological Research. doi: 10.3724/SP.J.1141.2012.05511
    [14] WU Xu-Jun, ZONG Wei, SUN Yong-Mei, HU Xin-Tian, MA Yuan-Ye, WANG Jian-Hong.  Morphine addiction in non-human primates using rhesus monkeys, Zoological Research. doi: 10.3724/SP.J.1141.2012.01089
    [15] XIA Hou-Jun, CHEN Ce-Shi.  Progress of non-human primate animal models of cancers, Zoological Research. doi: 10.3724/SP.J.1141.2011.01070
    [16] CHEN Yan-Mei, QIN Dong-Dong, JIANG Hui-Hui, HU Xin-Tian, MA Yuan-Ye.  Sleep disorder, a potential early diagnostic marker for psychiatric and neurodegenerative diseases, Zoological Research. doi: 10.3724/SP.J.1141.2011.01081
    [17] XIANG Ying, CHEN Song, LI Jun-hua, ZHONG Shan, WANG Wan-yu, XIONG Yu-lang, Chen.  Immunogenicity of Yunnan-cobra Venom Factor in Non-human Primates, Zoological Research. doi: 10.3724/SP.J.1141.200805511
    [18] ZHANG Peng*, WATANABE Kunio.  On the Husbandry and Welfare of Captive Non-human Primates, Zoological Research.
    [19] JI Wei-zhi, ZOU Ru-jin, SHANG En-yuan, MEN Hong-sheng, YANG Shang-chuan.  Application and Conservation of Non-Human Primates in Biomedical Research, Zoological Research.
    [20] JI Wei-zhi, YANG Shang-chuan, CHEN Jian-chung, ZOU Ru-jin, SHAN En-yuan.  In Vitro Fertilization and Embryo Transfer in Non-Human Primates, Zoological Research.
  • 加载中
计量
  • 文章访问数:  1308
  • HTML全文浏览量:  62
  • PDF下载量:  1981
  • 被引次数: 0
出版历程
  • 收稿日期:  2015-12-10
  • 修回日期:  2016-05-20
  • 刊出日期:  2016-07-18

Application of the genome editing tool CRISPR/Cas9 in non-human primates

doi: 10.13918/j.issn.2095-8137.2016.4.214
    基金项目:  the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13010000) and the National Natural Science Foundation of China (31130051)
    通讯作者: Bing SU

摘要: 近年来,CRISPR/Cas9基因编辑技术已经成功在多种实验动物中实现基因编辑。与小鼠等其他模式动物相比,非人灵长类在解剖、生理、行为和遗传上都与人类更接近。因此,非人灵长类在研究人类进化、人类认知功能、人类神经退行性疾病及药物筛选上具有巨大的优势,是理想的动物模型。目前,利用CRISPR/Cas9在非人灵长类基因编辑方面已经取得了一些进展,但是还存在很多问题。本文较为系统地总结了CRISPR/Cas9以及衍生的paCas9技术在非人灵长类基因编辑应用方面的进展,存在的问题以及改进的方向。

English Abstract

Xin LUO, Min LI, Bing SU. CRISPR/Cas9基因组编辑技术在非人灵长类中的应用[J]. 动物学研究, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214
引用本文: Xin LUO, Min LI, Bing SU. CRISPR/Cas9基因组编辑技术在非人灵长类中的应用[J]. 动物学研究, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214
Xin LUO, Min LI, Bing SU. Application of the genome editing tool CRISPR/Cas9 in non-human primates. Zoological Research, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214
Citation: Xin LUO, Min LI, Bing SU. Application of the genome editing tool CRISPR/Cas9 in non-human primates. Zoological Research, 2016, 37(4): 214-219. doi: 10.13918/j.issn.2095-8137.2016.4.214
参考文献 (45)

目录

    /

    返回文章
    返回