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Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change

Xin-Jiang LU Hao ZHANG Guan-Jun YANG Ming-Yun LI Jiong CHEN

Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change. Zoological Research, 2016, 37(3): 126-135. doi: 10.13918/j.issn.2095-8137.2016.3.126
Citation: Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change. Zoological Research, 2016, 37(3): 126-135. doi: 10.13918/j.issn.2095-8137.2016.3.126


doi: 10.13918/j.issn.2095-8137.2016.3.126

    Jiong CHEN

Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change

Funds: The project was supported by the Program for the National Natural Science Foundation of China (31201970) and the KC Wong Magna Fund in Ningbo University
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    Corresponding author: Jiong CHEN
  • 摘要: 香鱼(Plecoglossus altivelis)是一种东亚地区的洄游性鱼类,幼鱼生活在咸淡水(BW)中,成鱼生活在淡水(FW)中。本研究发现FW适应的香鱼幼鱼和BW适应的香鱼幼鱼相比,生长更为缓慢。然而FW适应抑制生长的机制不明。我们通过转录组测序研究FW适应以后香鱼幼鱼的基因表达变化。研究发现在FW适应组和咸淡水适应组相比,158个基因表达上调,而139个基因表达下调。通过GO基因功能富集分析和KEGG信号通路分析发现差异表达的基因和多种生物功能和过程相关,包括神经内分泌学、渗透压调节、能量代谢和细胞骨架。进一步采用荧光定量PCR验证差异表达基因在FW适应香鱼幼鱼的表达,发现碘化甲腺氨酸脱碘酶(ID)、促阿片-黑素细胞皮质素原(POMC)、甜菜碱半胱氨酸甲基转移酶(BHMT)、果糖二磷酸醛缩酶B(aldolase B)、酪氨酸转氨酸(TAT)和钠钾ATP酶(NKA)在香鱼幼鱼FW适应后表达上调,而b型钠尿肽(BNP)和转凝蛋白在香鱼幼鱼FW适应以后表达下调,这个结果和转录组测序的结果一致。我们的数据表明FW适应可能通过改变内分泌激素、能量代谢和直接的渗透压调控而减缓香鱼幼鱼生长。
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  • 收稿日期:  2015-12-02
  • 修回日期:  2016-04-29
  • 刊出日期:  2016-05-18