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Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

Qiang CHEN Xin-Jiang LU Ming-Yun LI Jiong CHEN

Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
Citation: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96

香鱼CSF-1R基因的克隆、病理相关性表达及功能分析

doi: 10.13918/j.issn.2095-8137.2016.2.96
详细信息
    通讯作者:

    Jiong CHEN

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

Funds: This project was supported by the Program for the National Natural Science Foundation of China (31372555), Zhejiang Provincial Natural Science Foundation of China (LZ13C190001), Scientific Research Foundation of Graduate School of Ningbo University (G15063), and KC Wong Magna Fund in Ningbo University
More Information
    Corresponding author: Jiong CHEN
  • 摘要: 巨噬细胞集落刺激因子受体(colony-stimulating factor 1 receptor,CSF-1R)是单核/巨噬细胞(MO/MΦ)上的重要调控因子。虽然CSF-1R基因在一些硬骨鱼中已被鉴定,但其功能尚不明确。本文对一种硬骨鱼类-香鱼(Plecoglossus altivelis)的CSF-1R基因进行了研究。多重序列比对和系统进化树分析表明,香鱼CSF-1R和青鳉CSF-1R的进化关系最近。实时荧光定量PCR检测揭示香鱼CSF-1R基因mRNA主要在头肾来源的单核/巨噬细胞(MO/MΦ)、脾和头肾中表达,鳗弧菌感染后,多个组织中CSF-1R基因mRNA表达显著变化。随后我们原核表达并制备了香鱼CSF-1R抗体。抗体封闭48小时后,流式细胞术检测香鱼MO/MΦ对大肠杆菌的吞噬能力显著降低,表明香鱼CSF-1R参与调控MO/MΦ功能。
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  • 收稿日期:  2015-10-22
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  • 刊出日期:  2016-03-18

Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

doi: 10.13918/j.issn.2095-8137.2016.2.96
    基金项目:  This project was supported by the Program for the National Natural Science Foundation of China (31372555), Zhejiang Provincial Natural Science Foundation of China (LZ13C190001), Scientific Research Foundation of Graduate School of Ningbo University (G15063), and KC Wong Magna Fund in Ningbo University
    通讯作者: Jiong CHEN

摘要: 巨噬细胞集落刺激因子受体(colony-stimulating factor 1 receptor,CSF-1R)是单核/巨噬细胞(MO/MΦ)上的重要调控因子。虽然CSF-1R基因在一些硬骨鱼中已被鉴定,但其功能尚不明确。本文对一种硬骨鱼类-香鱼(Plecoglossus altivelis)的CSF-1R基因进行了研究。多重序列比对和系统进化树分析表明,香鱼CSF-1R和青鳉CSF-1R的进化关系最近。实时荧光定量PCR检测揭示香鱼CSF-1R基因mRNA主要在头肾来源的单核/巨噬细胞(MO/MΦ)、脾和头肾中表达,鳗弧菌感染后,多个组织中CSF-1R基因mRNA表达显著变化。随后我们原核表达并制备了香鱼CSF-1R抗体。抗体封闭48小时后,流式细胞术检测香鱼MO/MΦ对大肠杆菌的吞噬能力显著降低,表明香鱼CSF-1R参与调控MO/MΦ功能。

English Abstract

Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 香鱼CSF-1R基因的克隆、病理相关性表达及功能分析[J]. 动物学研究, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
引用本文: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. 香鱼CSF-1R基因的克隆、病理相关性表达及功能分析[J]. 动物学研究, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
Citation: Qiang CHEN, Xin-Jiang LU, Ming-Yun LI, Jiong CHEN. Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis. Zoological Research, 2016, 37(2): 96-102. doi: 10.13918/j.issn.2095-8137.2016.2.96
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