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Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos

Fang DING Li CHEN Yong LIU Feng-Rui WU Biao DING Wen-Yong LI Rong WANG

Fang DING, Li CHEN, Yong LIU, Feng-Rui WU, Biao DING, Wen-Yong LI, Rong WANG. Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos. Zoological Research, 2015, 36(1): 54-58. doi: 10.13918/j.issn.2095-8137.2015.1.54
Citation: Fang DING, Li CHEN, Yong LIU, Feng-Rui WU, Biao DING, Wen-Yong LI, Rong WANG. Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos. Zoological Research, 2015, 36(1): 54-58. doi: 10.13918/j.issn.2095-8137.2015.1.54

Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos

doi: 10.13918/j.issn.2095-8137.2015.1.54
基金项目: This study was supported by the National Natural Science Foundation of China (31372273, 31201789); Academic Renovation Research Project of Anhui University (yqh100125); Natural Science Foundation of the Education Bureau of Anhui Province (KJ2013A202); Major project of discipline construction in Anhui province ([2014]No.28); and Natural Science Foundation of Anhui Province (1408085MC44, 1408085QC65)
详细信息
    通讯作者:

    Rong WANG

Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos

Funds: This study was supported by the National Natural Science Foundation of China (31372273, 31201789); Academic Renovation Research Project of Anhui University (yqh100125); Natural Science Foundation of the Education Bureau of Anhui Province (KJ2013A202); Major project of discipline construction in Anhui province ([2014]No.28); and Natural Science Foundation of Anhui Province (1408085MC44, 1408085QC65)
More Information
    Corresponding author: Rong WANG
  • 摘要: It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels (except at the 2-cell stage). This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early pre-implantation mouse embryos.
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出版历程
  • 收稿日期:  2014-07-14
  • 修回日期:  2014-10-25
  • 刊出日期:  2015-01-08

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