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Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

Gui LI Ren LAI Gang DUAN Long-Bao LYU Zhi-Ye ZHANG Huang LIU Xun XIANG

Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492
Citation: Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492

Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

doi: 10.13918/j.issn.2095-8137.2014.6.492
基金项目: This study was supported by the National 863 Project of China ( 2012AA021801) and the Project of Frontier Study of Foundation, CAS (KSCX2-EW-R-11, KSCX2-EW-J-23)
详细信息
    通讯作者:

    Ren LAI

Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

Funds: This study was supported by the National 863 Project of China ( 2012AA021801) and the Project of Frontier Study of Foundation, CAS (KSCX2-EW-R-11, KSCX2-EW-J-23)
More Information
    Corresponding author: Ren LAI
  • 摘要: Endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews (Tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis (detection rate 42%), Pseudomonas aeruginosa (25%), Staphlococcus aureus (33%), S. Epidermidis (75%), Micrococcus luteus (25%), Kurthia gibsonii (17%); oral cavity: Neisseria mucosa (58%), Streptococcus pneumonia (17%); anus: Enterococcus faecalis (17%), Lactococus lactis (33%), Escherichia coli (92%), Salmonella typhosa (17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis (75%); oral cavity: N.mucosa (67%); anus: L. lactis (33%), E. coli (100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.
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出版历程
  • 收稿日期:  2014-03-23
  • 修回日期:  2014-06-25
  • 刊出日期:  2014-11-08

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