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Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles

Li WEI Wei-Wei SHAO Guo-Hua DING Xiao-Li FAN Miao-Ling YU Zhi-Hua LIN

Li WEI, Wei-Wei SHAO, Guo-Hua DING, Xiao-Li FAN, Miao-Ling YU, Zhi-Hua LIN. Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles. Zoological Research, 2014, 35(4): 272-279. doi: 10.13918/j.issn.2095-8137.2014.4.272
Citation: Li WEI, Wei-Wei SHAO, Guo-Hua DING, Xiao-Li FAN, Miao-Ling YU, Zhi-Hua LIN. Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles. Zoological Research, 2014, 35(4): 272-279. doi: 10.13918/j.issn.2095-8137.2014.4.272

Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles

doi: 10.13918/j.issn.2095-8137.2014.4.272
基金项目: This work was supported by the National Natural Science Foundation of China (31270443) and the Natural Science Foundation of Zhejiang Province (LY13C030004).
详细信息
    通讯作者:

    Zhi-Hua LIN

Acute and joint toxicity of three agrochemicals to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles

  • 摘要: We studied acute and joint toxicity of three different agrochemicals (chlorantraniliprole, flubendiamide-abamectin and penoxsulam) to Chinese tiger frog (Hoplobatrachus chinensis) tadpoles with the method of stability water tests. Results showed that the three agrochemicals increased tadpole mortality. For acute toxicity, the LC50 values after 24, 48 and 72 h of chlorantraniliprole, flubendiamide-abamectin and penoxsulam exposure were 5.37, 4.90 and 4.68 mg/L; 0.035, 0.025 and 0.021 mg/L; 1.74, 1.45 and 1.29 mg/L, respectively. The safety concentrations (SC) of chlorantraniliprole, flubendiamide-abamectin and penoxsulam to the tadpoles were 1.23, 0.30 and 0.003 mg/L, respectively. Based on these findings, chlorantraniliprole and penoxsulam were moderately toxic, while flubendiamide-abamectin was highly toxic. All pairwise joint toxicity tests showed moderate toxicity. The LC50 values after 24, 48 and 72 h of exposure were 7.08, 6.61 and 6.03 mg/L for chlorantraniliprole+penoxsulam, with corresponding values of 2.455, 2.328 and 2.183 mg/L for chlorantraniliprole+flubendiamide-abamectin, and 1.132, 1.084 and 1.050 mg/L for penoxsulam+flubendiamide-abamectin, with safe concentrations of 1.73, 0.63 and 0.30 mg/L, respectively. For toxic evaluations of pairwise combinations of the three agrochemicals, only the joint toxicity of chlorantraniliprole and flubendiamide-abamectin after 24 h was found to be synergistic, whereas all other tests were antagonistic. Our findings provide valuable information on the toxic effects of agrochemicals on amphibians and how various types of agrochemicals can be reasonably used in agricultural areas.
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  • 收稿日期:  2013-12-04
  • 修回日期:  2014-02-20
  • 刊出日期:  2014-07-08

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