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Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)

Ai-Hua LEI Gao-Hong ZHANG Ren-Rong TIAN Jia-Wu ZHU Hong-Yi ZHENG Wei PANG Yong-Tang ZHENG

Ai-Hua LEI, Gao-Hong ZHANG, Ren-Rong TIAN, Jia-Wu ZHU, Hong-Yi ZHENG, Wei PANG, Yong-Tang ZHENG. Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina). Zoological Research, 2014, 35(3): 186-195. doi: 10.11813/j.issn.0254-5853.2014.3.186
Citation: Ai-Hua LEI, Gao-Hong ZHANG, Ren-Rong TIAN, Jia-Wu ZHU, Hong-Yi ZHENG, Wei PANG, Yong-Tang ZHENG. Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina). Zoological Research, 2014, 35(3): 186-195. doi: 10.11813/j.issn.0254-5853.2014.3.186

Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)

doi: 10.11813/j.issn.0254-5853.2014.3.186
基金项目: This work was supported by the National Basic Research Program (2012CBA01305); the National Natural Science Foundation of China (81172876, U0832601, 81273251 and U1202228); the Knowledge Innovation Program of CAS (KSCX2-EW-R-13, Y206A71181), and the Key Scientific and Technological Program of China (2012ZX10001-007, 2013ZX10001-002).
详细信息
    通讯作者:

    Wei PANG, Yong-Tang ZHENG

Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)

  • 摘要: The northern pig-tailed macaque (Macaca leonina) has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus type 1 (HIV-1), which may be due to the absence of a TRIM5 protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M. leonina. The results indicate that these strains are characterized by various but low replication levels, and among which, HIV-1NL4-3 shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of vif-substituted HIV-1 (HSIV) in M. leonina PBMCs. Notably, HSIV-vifmac and stHIV-1SV chimeras, two HIV-1NL4-3-derived viruses encoding the viral infectivity factor (Vif) protein from SIVmac239, replicated robustly in cells from M. leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M. leonina. Therefore, our data demonstrate that M. leonina has the potential to be developed into a promising animal model for human AIDS.
  • [1] Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, Martin MA. 1986. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. Journal of Virology, 59(2): 284-291.
    [2] Agy MB, Frumkin LR, Corey L, Coombs RW, Wolinsky SM, Koehler J, Morton WR, Katze MG. 1992. Infection of Macaca nemestrina by human immunodeficiency virus type-1. Science, 257(5066): 103-106.
    [3] Agy MB, Schmidt A, Florey MJ, Kennedy BJ, Schaefer G, Katze MG, Corey L, Morton WR, Bosch ML. 1997. Serial in vivo passage of HIV-1 infection in Macaca nemestrina. Virology, 238(2): 336-343.
    [4] Ahn K, Huh JW, Park SJ, Kim DS, Ha HS, Kim YJ, Lee JR, Chang KT, Kim HS. 2008. Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues. BMC Molecular Biology, 9: 78.
    [5] Albin JS, Harris RS. 2010. Interactions of host APOBEC3 restriction factors with HIV-1 in vivo: implications for therapeutics. Expert Reviews in Molecular Medicine, 12: e4.
    [6] Aldovini A, Walker BD. 1990. Techniques in HIV Research. New York: Stockton Press.
    [7] Ambrose Z, KewalRamani VN, Bieniasz PD, Hatziioannou T. 2007. HIV/AIDS: in search of an animal model. Trends in Biotechnology, 25(8): 333-337.
    [8] Arhel NJ, Nisole S, Carthagena L, Coutant F, Souque P, Brussel A, Estaquier J, Charneau P. 2008. Lack of endogenous TRIM5α-mediated restriction in rhesus macaque dendritic cells. Blood, 112(9): 3772-3776.
    [9] Baroncelli S, Negri DR, Michelini Z, Cara A. 2008. Macaca mulatta, fascicularis and nemestrina in AIDS vaccine development. Expert Review of Vaccines, 7(9): 1419-1434.
    [10] Batten CJ, De Rose R, Wilson KM, Agy MB, Chea S, Stratov I, Montefiori DC, Kent SJ. 2006. Comparative evaluation of simian, simian-human, and human immunodeficiency virus infections in the pigtail macaque (Macaca nemestrina) model. AIDS Research and Human Retroviruses, 22(6): 580-588.
    [11] Blanco-Melo D, Venkatesh S, Bieniasz PD. 2012. Intrinsic cellular defenses against human immunodeficiency viruses. Immunity, 37(3): 399-411.
    [12] Bosch ML, Schmidt A, Chen JL, Florey MJ, Agy M, Morton WR. 2000. Enhanced replication of HIV-1 in vivo in pigtailed macaques (Macaca nemestrina). Journal of Medical Primatology, 29(3-4): 107-113.
    [13] Brennan G, Kozyrev Y, Hu SL. 2008. TRIMCyp expression in Old World primates Macaca nemestrina and Macaca fascicularis. Proceedings of the National Academy of Sciences of the United States of America, 105(9): 3569-3574.
    [14] Brennan G, Kozyrev Y, Kodama T, Hu SL. 2007. Novel TRIM5 isoforms expressed by Macaca nemestrina. Journal of Virology, 81(22): 12210-12217.
    [15] Dai ZX, Zhang GH, Zhang XH, Zheng YT. 2013. Identification and characterization of a novel splice variant of rhesus macaque MHC IA. Molecular Immunology, 53(3): 206-213.
    [16] Groves CP. 2001. Primate Taxonomy. Washington DC: Smithsonian Institution Press, 222-224.
    [17] Evans DT, Silvestri G. 2013. Nonhuman primate models in AIDS research. Current Opinion in HIV and AIDS, 8(4): 255-261.
    [18] Gaddis NC, Sheehy AM, Ahmad KM, Swanson CM, Bishop KN, Beer BE, Marx PA, Gao F, Bibollet-Ruche F, Hahn BH, Malim MH. 2004. Further investigation of simian immunodeficiency virus Vif function in human cells. Journal of Virology, 78(21): 12041-12046.
    [19] Gartner S, Liu YL, Polonis V, Lewis MG, Elkins WR, Hunter EA, Miao J, Corts KJ, Eddy GA. 1994. Adaptation of HIV-1 to Pigtailed Macaques. Journal of Medical Primatology, 23(2-3): 155-163.
    [20] Gippoliti S. 2001. Notes on the taxonomy of Macaca nemestrina leonina blyth, 1863 (Primates: Cercopithecidae). Hystrix-Italian Journal of Mammalogy, 12(1): 51-54.
    [21] Hatziioannou T, Evans DT. 2012. Animal models for HIV/AIDS research. Nature Reviews Microbiology, 10(12): 852-867.
    [22] Hatziioannou T, Princiotta M, Piatak M, Jr., Yuan F, Zhang FW, Lifson JD, Bieniasz PD. 2006. Generation of simian-tropic HIV-1 by restriction factor evasion. Science, 314(5796): 95.
    [23] Hatziioannou T, Ambrose Z, Chung NPY, Piatak M Jr, Yuan F, Trubey CM, Coalter V, Kiser R, Schneider D, Smedley J, Pung R, Gathuka M, Estes JD, Veazey RS, KewalRamani VN, Lifson JD, Bieniasz PD. 2009. A macaque model of HIV-1 infection. Proceedings of the National Academy of Sciences of the United States of America, 106(11): 4425-4429.
    [24] Hu SL. 2005. Non-human primate models for AIDS vaccine research. Current Drug Targets-Infectious Disorders, 5(2): 193-201.
    [25] Huang N, Wu MY, Zheng CB, Zhu L, Zhao JH, Zheng YT. 2013. The depolymerized fucosylated chondroitin sulfate from sea cucumber potently inhibits HIV replication via interfering with virus entry. Carbohydrate Research, 380: 64-69.
    [26] Humes D, Overbaugh J. 2011. Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells. Journal of Virology, 85(9): 4409-4420.
    [27] Huthoff H, Towers GJ. 2008. Restriction of retroviral replication by APOBEC3G/F and TRIM5α. Trends in Microbiology, 16(12): 612-619.
    [28] Igarashi T, Iyengar R, Byrum RA, Buckler-White A, Dewar RL, Buckler CE, Lane HC, Kamada K, Adachi A, Martin MA. 2007. Human immunodeficiency virus type 1 derivative with 7% simian immunodeficiency virus genetic content is able to establish infections in pig-tailed macaques. Journal of Virology, 81(20): 11549-11552.
    [29] Kamada K, Yamashita T, Hatcho K, Adachi A, Nomaguchi M. 2009. Evasion from CypA-and APOBEC-mediated restrictions is insufficient for HIV-1 to efficiently grow in simian cells. Microbes and Infection, 11(2): 164-171.
    [30] Kamada K, Igarashi T, Martin MA, Khamsri B, Hatcho K, Yamashita T, Fujita M, Uchiyama T, Adachi A. 2006. Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells. Proceedings of the National Academy of Sciences of the United States of America, 103(45): 16959-16964.
    [31] Kent SJ, Corey L, Agy MB, Morton WR, McElrath MJ, Greenberg PD. 1995. Cytotoxic and proliferative T cell responses in HIV-1-infected Macaca nemestrina. The Journal of Clinical Investigation, 95(1): 248-256.
    [32] Klatt NR, Canary LA, Vanderford TH, Vinton CL, Engram JC, Dunham RM, Cronise HE, Swerczek JM, Lafont BA, Picker LJ, Silvestri G, Brenchley JM. 2012. Dynamics of simian immunodeficiency virus SIVmac239 infection in pigtail macaques. Journal of Virology, 86(2): 1203-1213.
    [33] Kuang YQ, Tang X, Liu FL, Jiang XL, Zhang YP, Gao GX, Zheng YT. 2009. Genotyping of TRIM5 locus in northern pig-tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection. Retrovirology, 6(1): 58.
    [34] Kuller L, Schmidt A, Mack H, Durning M, Birkebak T, Reiner MT, Anderson DM, Morton WR, Agy MB. 2001. Systemic and intestinal immune responses to HIV-2287 infection in Macaca nemestrina. AIDS Research and Human Retroviruses, 17(12): 1191-1204.
    [35] Kuroishi A, Saito A, Shingai Y, Shioda T, Nomaguchi M, Adachi A, Akari H, Nakayama EE. 2009. Modification of a loop sequence between α-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vif and CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells. Retrovirology, 6(1): 70.
    [36] Lackner AA, Veazey RS. 2007. Current concepts in AIDS pathogenesis: insights from the SIV/macaque model. Annual Review of Medicine, 58: 461-476.
    [37] Lei AH, Pang W, Zhang GH, Zheng YT. 2013. Use and research of pigtailed macaques in nonhuman primate HIV/AIDS Models. Zoological Research, 34(2): 77-88.
    [38] Li MH, Li SY, Xia HJ, Wang L, Wang YY, Zhang GH, Zheng YT. 2007. Establishment of AIDS animal model with SIVmac239 infected Chinese rhesus monkey. Virologica Sinica, 22(6): 509-516.
    [39] Liao CH, Kuang YQ, Liu HL, Zheng YT, Su B. 2007. A novel fusion gene, TRIM5-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection. AIDS, 21(Suppl 8): S19-S26.
    [40] Liu HL, Wang YQ, Liao CH, Kuang YQ, Zheng YT, Su B. 2005. Adaptive evolution of primate TRIM5α, a gene restricting HIV-1 infection. Gene, 362: 109-116.
    [41] Malaivijitnond S, Arsaithamkul V, Tanaka H, Pomchote P, Jaroenporn S, Suryobroto B, Hamada Y. 2012. Boundary zone between northern and southern pig-tailed macaques and their morphological differences. Primates, 53(4): 377-389.
    [42] Mariani R, Chen D, Schröfelbauer B, Navarro F, König R, Bollman B, Münk C, Nymark-McMahon H, Landau NR. 2003. Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. Cell, 114(1): 21-31.
    [43] Newman RM, Hall L, Kirmaier A, Pozzi LA, Pery E, Farzan M, O'Neil SP, Johnson W. 2008. Evolution of a TRIM5-CypA splice isoform in old world monkeys. PLoS Pathogens, 4(2): e1000003.
    [44] Nomaguchi M, Doi N, Fujiwara S, Saito A, Akari H, Nakayama EE, Shioda T, Yokoyama M, Sato H, Adachi A. 2013a. Systemic biological analysis of the mutations in two distinct HIV-1mt genomes occurred during replication in macaque cells. Microbes and Infection, 15(4): 319-328.
    [45] Nomaguchi M, Yokoyama M, Kono K, Nakayama EE, Shioda T, Saito A, Akari H, Yasutomi Y, Matano T, Sato H, Adachi A. 2013b. Gag-CA Q110D mutation elicits TRIM5-independent enhancement of HIV-1mt replication in macaque cells. Microbes and Infection, 15(1): 56-65.
    [46] Otten RA, Ellenberger DL, Adams DR, Fridlund CA, Jackson E, Pieniazek D, Rayfield MA. 1999. Identification of a window period for susceptibility to dual infection with two distinct human immunodeficiency virus type 2 isolates in a Macaca nemestrina (pig-tailed macaque) model. The Journal of Infectious Diseases, 180(3): 673-684.
    [47] Rosenblum LL, Supriatna J, Melnick DJ. 1997. Phylogeographic analysis of pigtail macaque populations (Macaca nemestrina) inferred from mitochondrial DNA. American Journal of Physical Anthropology, 104(1): 35-45.
    [48] Saito A, Nomaguchi M, Iijima S, Kuroishi A, Yoshida T, Lee YJ, Hayakawa T, Kono K, Nakayama EE, Shioda T, Yasutomi Y, Adachi A, Matano T, Akari H. 2011. Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications. Microbes and Infection, 13(1): 58-64.
    [49] Sheehy AM, Gaddis NC, Choi JD, Malim MH. 2002. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature, 418(6898): 646-650.
    [50] Shibata R, Kawamura M, Sakai H, Hayami M, Ishimoto A, Adachi A. 1991. Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. Journal of Virology, 65(7): 3514-3520.
    [51] Stopak K, de Noronha C, Yonemoto W, Greene WC. 2003. HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability. Molecular Cell, 12(3): 591-601.
    [52] Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P, Sodroski J. 2004. The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys. Nature, 427(6977): 848-853.
    [53] Stremlau M, Perron M, Lee M, Li Y, Song B, Javanbakht H, Diaz-Griffero F, Anderson DJ, Sundquist WI, Sodroski J. 2006. Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5α restriction factor. Proceedings of the National Academy of Sciences of the United States of America, 103(14): 5514-5519.
    [54] Thippeshappa R, Ruan H, Kimata JT. 2012. Breaking barriers to an AIDS model with macaque-tropic HIV-1 derivatives. Biology (Basel), 1(2): 134-164.
    [55] Thippeshappa R, Polacino P, Kimata MTY, Siwak EB, Anderson D, Wang WM, Sherwood L, Arora R, Wen M, Zhou P, Hu SL, Kimata JT. 2011. Vif substitution enables persistent infection of pig-tailed macaques by human immunodeficiency virus type 1. Journal of Virology, 85(8): 3767-3779.
    [56] Virgen CA, Hatziioannou T. 2007. Antiretroviral activity and Vif sensitivity of rhesus macaque APOBEC3 proteins. Journal of Virology, 81(24): 13932-13937.
    [57] Virgen CA, Kratovac Z, Bieniasz PD, Hatziioannou T. 2008. Independent genesis of chimeric TRIM5-cyclophilin proteins in two primate species. Proceedings of the National Academy of Sciences of the United States of America, 105(9): 3563-3568.
    [58] Wang RR, Gao YD, Ma CH, Zhang XJ, Huang CG, Huang JF, Zheng YT. 2011. Mangiferin, an anti-HIV-1 agent targeting protease and effective against resistant strains. Molecules, 16(5): 4264-4277.
    [59] Zennou V, Bieniasz PD. 2006. Comparative analysis of the antiretroviral activity of APOBEC3G and APOBEC3F from primates. Virology, 349(1): 31-40.
    [60] Zhang GH, Li MH, Zheng YT. 2007. Application of AIDS macaque animal model in HIV vaccine research. Zoological Research, 28(5): 556-562.
    [61] Zhang XJ, Yang GY, Wang RR, Pu JX, Sun HD, Xiao WL, Zheng YT. 2010. 7,8-secolignans from Schisandra wilsoniana and their anti-HIV-1 activities. Chemistry & Biodiversity, 7(11): 2692-2701.
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