Volume 22 Issue 5
Sep.  2001
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ZHA Hong-Guang, ZHANG Yun. Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus. Zoological Research, 2001, 22(5): 392-396.
Citation: ZHA Hong-Guang, ZHANG Yun. Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus. Zoological Research, 2001, 22(5): 392-396.

Cloning of cDNAs Encoding Two Novel Phospholipase A[2]s from Bungarus fasciatus

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-10-22
  • The total mRNA was prepared and purified from the venom glands of snake Bungarus fasciatus.A cDNA library of the venom glands was then constructed using reverse transcription.Two cDNAs encoding two novel phospholipase A[2],named as Bungarus fasciatus phospholipase A[2]Ⅰ(Bf-PLA[2]Ⅰ) and Bungarus fasciatus phospholipase A[2] Ⅱ(Bf-PLA[2]Ⅱ) respectively,were screened out by polymerase chain reaction and sequenced.Both cDNAs have an open reading frame of 435 bp,encoding a 145 amino acid phospholipase A2 precursor composed of a 27 amino acid signal peptide followed by 118 amino acid mature protein.The two cloned phospholipase A[2] belong to Group Ⅰ phospholipase A[2].Their primary structures are different from known Bungarus fasciatus phospholipase A[2]s.
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