Volume 22 Issue 4
Jul.  2001
Turn off MathJax
Article Contents
ZHOU Li, LIU Jin-Xia, GUI Jian-Fang. Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA. Zoological Research, 2001, 22(4): 257-264.
Citation: ZHOU Li, LIU Jin-Xia, GUI Jian-Fang. Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA. Zoological Research, 2001, 22(4): 257-264.

Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-08-22
  • 24 individuals from five different gynogenetic strains of silver crucian carp (Carassius auratus gibelio Bloch) were analyzed using 8 microsatellite markers of the poly (CA) type isolated from common carp (Cyprinus carpio L.) by Crooijmans et al.(1997).Electrophoretogram patterns showed that 7 pairs of microsatellite primers reproducibly produced the well-identifiable and homologous DNA fragments except the primers of MFW28.The number of alleles per marker varied from 1 to 14,and the size of alleles ranged from 100 bp to 506 bp.Nearly identical banding patterns occurred among intra-strain individuals,although subtle polymorphism was occasionally detected in the five strains.It was suggested that each strain might possess specific pattern owing to its gynogenesis.On other hand,the five strains differed from each other in electrophoretogram patterns.A total of 23 polymorphic fragments were scored from 5 pairs of primers after excluding bands that were monomorphic for the five strains.The electrophoretogram pattern clearly indicated their intra-strain homogeneity,inter-strain heterogeneity,and their phylogenetic relationships.Strain P is same to strain A,and strain F originates from strain E.Strains A,D and E may originate from different multiple events of hybridization respectively.The microsatellite markers produced by this study will be of benefit to further studies on the evolutionary genetics and provide useful tools for marker-assisted selection breeding or genetic map.
  • loading
  • Relative Articles

    [1] Jing Yang, Guo-Fen Zhu, Jian Jiang, Chang-Lin Xiang, Fu-Li Gao, Wei-Dong Bao. Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management. Zoological Research, 2019, 40(5): 439-448.  doi: 10.24272/j.issn.2095-8137.2019.058
    [2] Jin-Jin MIN, Rong-Hui YE, Gen-Fang ZHANG, Rong-Quan ZHENG. Microsatellite analysis of genetic diversity and population structure of freshwater mussel (Lamprotula leai). Zoological Research, 2015, 36(1): 34-40.  doi: 10.13918/j.issn.2095-8137.2015.1.34
    [3] Ya HE, Zheng-Huan WANG, Xiao-Ming WANG. Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation. Zoological Research, 2014, 35(6): 528-536.  doi: 10.13918/j.issn.2095-8137.2014.6.528
    [4] Ji-Min MA, You-Yi KUANG, Xian-Hu ZHENG, Ding-Chen CAO, Wei-Hua LYU, Yu CHANG, Dan ZHOU, Ke DU, Xiao-Wen SUN. Screening and body correlation analysis of microsatellite markers related to intermuscular bone number in common carp (Cyprinus carpio). Zoological Research, 2013, 34(4): 406-410.  doi: 10.11813/j.issn.0254-5853.2013.4.0406
    [5] Min TAO, Zhen-Yan SONG, Jun XIAO, Shao-Jun LIU, Kai-Kun LUO, Tuo-Mi ZOU, Jun WANG, Wei LIU, Jie HU, Ru-Rong ZHAO, Yun LIU. Cytogenetic and molecular genetic analysis of gynogenesis in Megalobrama amblycephala using spermatozoa of Erythroculter ilishaeformis. Zoological Research, 2013, 34(5): 479-486.  doi: 10.11813/j.issn.0254-5853.2013.5.0479
    [6] LIU Chang-Jing, ZHAO Wei, ZHOU Rong, LIU Nai-Fa. Phylogeny and genetic diversity of Phrynocephalus przewalskii. Zoological Research, 2012, (2): 127-132.  doi: 10.3724/SP.J.1141.2012.02127
    [7] Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI. Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis. Zoological Research, 2012, 33(E3-4): 33-39.  doi: 10.3724/SP.J.1141.2012.E03-04E33
    [8] MA Ke-Yi, FENG Jian-Bin, XIE Nan, FENG Xiao-Yu, LI Jia-Le. Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River. Zoological Research, 2011, 32(4): 363-370.  doi: 10.3724/SP.J.1141.2011.04363
    [9] LIU Bo, KUANG You-Yi, TONG Guang-Xiang, YIN Jia-Sheng. Analysis of genetic diversity on 9 wild stocks of Taimen (Hucho taimen) by microsatellite markers. Zoological Research, 2011, 32(6): 597-604.  doi: 10.3724/SP.J.1141.2011.06597
    [10] LIN Fang-Jun, JIANG Ping-Ping, DING Ping. Genetic analysis of microsatellite polymorphism in the Elliot’s Pheasant (Syrmaticus ellioti) in China. Zoological Research, 2010, 31(5): 461-468.  doi: 10.3724/SP.J.1141.2010.05461
    [11] LIU Yan-hua, ZHANG Ming-hai. Population Genetic Diversity of Roe Deer (Capreolus pygargus) in Mountains of Heilongjiang Province. Zoological Research, 2009, 30(2): 113-120.  doi: 10.3724/SP.J.1141.2009.02113
    [12] HUO Jin-long, HUO Hai-long, MIAO Yong-wang, LI Fu-quan, LIU Li-xian, WU Ge-min, OUYAN. Genetic Diversity of 76 STR Loci in the Dahe Pig. Zoological Research, 2009, 30(1): 105-108.  doi: 10.3724/SP.J.1141.2009.01105
    [13] JI Xu, SUN Xiao-wen, YANG Li-geng, Li Ya-juan, LIU Hai-jin. Paternity Testing of Mitogynogenetic Japanese Flounder (Paralichthys olivaceus) Using Microsatellite Loci. Zoological Research, 2008, 29(1): 25-30.
    [14] LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu. Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers. Zoological Research, 2008, 29(1): 31-36.
    [15] YE Lang-hui HUO Jin-long MIAO Yong-wang , * ZHU Sheng-quan CHEN Tao LIU Li-xian PAN Wei-rong BI Bao-liang. Genetic Diversity Analysis of Nixi Chicken Using Microsatellite DNA Markers. Zoological Research, 2006, 27(1): 68-74.
    [16] ZHU Xiao-chen LIU Hai-jin , * SUN Xiao-wen XUE Ling-ling MAO Lian-ju. Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus). Zoological Research, 2006, 27(1): 63-67.
    [17] ZHANG Yan-shuai, FAN Xue-ming. Genetic Diversity of Hydra robusta in Heilongjiang Province by RAPD Analysis. Zoological Research, 2005, 26(2): 152-156.
    [18] SHEN Wen-ying, ZHANG Li-hong, ZHENG Yong-ping, ZHOU Liu-qin, ZHENG Jian-ping. Effects of Starvation on Blood Composition and Ovarian Development in Carassius auratus gibelio. Zoological Research, 2003, 24(6): 441-444.
    [19] LU Cheng, ZHAO Ai-chun, XIANG Zhong-huai, WAN Chun-ling. AFLP Analysis of Genetic Diversity of Bombyx mandarina in China. Zoological Research, 2002, 23(2): 166-172.
    [20] LIU Jing-xia, ZHOU Li, ZHAO Zhen-shan, GUI Jian-fang. Studies on Microsatellite Markers of Four Artificially Gynogenetic Families in Ornamental Carp. Zoological Research, 2002, 23(2): 97-105.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2296) PDF downloads(1683) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint